Luận án Nghiên cứu tạo chủng vi khuẩn vibrio parahaemolyticus đột biến giảm độc lực nhằm phát triển vắc - Xin phòng bệnh hoại tử gan thận trên một số loài cá biển

GAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B5700 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC 147 ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B7050 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTATACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC 148 AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCTTTCCAGAAGTGAAAGCGGAATATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCAACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B7900 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTTAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT 149 GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA Phụ lục 3. Trình tự chuỗi polypeptide suy diễn từ gen tlh các chủng/dòng vi khuẩn Chủng/ dòng Trình tự polypeptide suy diễn các chủng/dòng vi khuẩn LBT6 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* L3900 MKKTITLLTALFPVASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* L4200 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV 150 DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NKLAAVPFI L4650 MKKTIPLLTALLPLASAVAEKPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQIIKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTVPDVT*APQFKYSPQEEIDKIREKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* A3.3 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* A400 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEDHGFVNASDPCLDINRSSS VNYMDTHALRSECAASGAEKFVFWDVTHPTTATHLYVAEKMLES SNNLAEYRF* A650 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA 151 MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPKNTVS*TQVILVWTSTAHRL SITCTPTHCALSVRRPVLRNLCSGMSRTQQQQLTAMLQRKC*KVAT T*HE*PFN B3.13 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* B7050 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMLY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGFPEVKAEYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* B7900 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGFNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS 152 NNLAEYRF* Phụ lục 4: Trình tự nucleotide đoạn gen rpoB của chủng/dòng vi khuẩn Chủng/ dòng Trình tự nucleotide đoạn gen rpoB của chủng/dòng vi khuẩn LBT6 AGCCACCAACAAAAGAAGCTGCAGAAGCACTATTCGAAAGCCT ATTCTTCTCTGAAGAGCGTTACGATCTATCGACTGTTGGTCGTAT GAAGTTTAATAGCTCTATCGGTCGTGAAGATGCTCAAGAGCAAG GCACACTTGATGAAACAGATATCATCGAAGTGATGAAGAAGCTT ATCGCGATCCGTAACGGCAAAGGCGAAGTGGACGATATCGACCA CCTAGGCAACCGTCGTATCCGTTCTGTCGGCGAAATGGCTGAGAA CCAGTTCCGTGTAGGTCTAGTACGTGTTGAGCGTGCAGTTAAAGA GCGTCTAAGCCTAGGCGATCTTGACGCAATCATGCCTCAAGACCT GATCAACGCGAAGCCAATTTCTGCAGCGGTTAAAGAATTCTTTGG CTCTTCACAGCTTTCACAGTTCATGGACCAAAACAACCCGCTGTC AGAAGTAACGCACAAGCGTCGTATTTCTGCATTGGGTCCTGGCGG TCTGACTCGTGAACGTGCTGGCTTCGAAGTTCGAGACGTACACGT AACTCACTACGGTCGTCTATGTCCTATCGAAACGCCGGAAGGTCC AAACATCGGTCTGATCAACTCTCTATCTGCGTTTGCGCGTTGTAA CGAGTACGGTTTCCTTGAGACGCCATACCGTCGCGTTGTAGATGG TGTTGTAACAGACGAAGTAGATTACCTATCTGCAATCGAAGAAG GCCAATTCGTTATCGCTCAGGCGAACGCTAAGCTAAACGAAGAT GGTACTTTTGCAGATGAGCTGATCACAGCTCGTCAGAAAGGTGA ATCTGGTCTACACCCTCGTGAGCACGCTCAGTACATGGACGTTGC GACAAACCAGGTTGTATCTATCGCTGCATCGCTTATCCCGTTCCT AGAACACGATGATGCGAACCGTGCATTGATGGGTGCGA L4650 ACTATTCGAAAACCTGTTCTTCTCCGAAGACCGCTACGATCTCTC CGCTGTTGGTCGTATGAAGTTCAACAGCTCTATCGGTCGTGACG ATGCTGAAGAGCAAGGTACACTGGATAAAGAAGACATCATCGA AGTGATGAAGAAGCTCATCGATATCCGTAACGGTAAAGGCGAA GTGGACGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGT AGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTG TAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT GCAATCATGCCTCAGGATATGATCAACGCCAAGCCGATCTCCGC AGCAGTGAAAGAGTTCTTTGGTTCCAGCCAGCTGTCTCAGTTTAT GGACCAGAACAACCCGCTGTCTGAGATCACGCACAAGCGTCGTA TCTCTGCACTCGGCCCAGGCGGTCTGACCCGTGAGCGCGCAGGC 153 TTCGAAGTTCGAGACGTACACGTAACCCACTACGGTCGCGTATG TCCAATCGAAACGCCTGAAGGTCCAAACATCGGTCTGATCAACT CCCTGTCCGTGTACGCACGTTGTAACGAATACGGCTTCCTCGAG ACCCCGTACCGTCGCGTGGTAGACGGTGTTGTAACCGACGAAGT ACACTACCTGTCTGCTATCGAAGAAGGTCAATACGTTATCGCCC AGGCGAACACCAACCTGGACGAAGAAGGTACTTTTGCAGATGA CCTCGTTACAGCCCGTCAGAAAGGTGAATCCAGCTTACTCCCTC GTGACCAGGTTCAGTACATGGACGTTTCCACCAACCAGGTGGTT TCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGC CAACC B3.13 ATCTACCGTATGATGCGCCCTGGTGAGCCACCAACAAAAGAAGC TGCAGAAGCACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTT ACGATCTATCGACTGTTGGTCGTATGAAGTTTAATAGCTCTATCG GTCGTGAAGATGCTCAAGAGCAAGGCACACTTGATGAAACAGA TATCATCGAAGTGATGAAGAAGCTTATCGCGATCCGTAACGGCA AAGGCGAAGTGGACGATATCGACCACCTAGGCAACCGTCGTATC CGTTCTGTCGGCGAAATGGCTGAGAACCAGTTCCGTGTAGGTCT AGTACGTGTTGAGCGTGCAGTTAAAGAGCGTCTAAGCCTAGGCG ATCTTGACGCAATCATGCCTCAAGACCTGATCAACGCGAAGCCA ATTTCTGCAGCGGTTAAAGAATTCTTTGGCTCTTCACAGCTTTCA CAGTTCATGGACCAAAACAACCCGCTGTCAGAAGTAACGCACAA GCGTCGTATTTCTGCATTGGGTCCTGGCGGTCTGACTCGTGAACG TGCTGGCTTCGAAGTTCGAGACGTACACGTAACTCACTACGGTC GTCTATGTCCTATCGAAACGCCGGAAGGTCCAAACATCGGTCTG ATCAACTCTCTATCTGCGTTTGCGCGTTGTAACGAGTACGGTTTC CTTGAGACGCCATACCGTCGCGTTGTAGATGGTGTTGTAACAGA CGAAGTAGATTACCTATCTGCAATCGAAGAAGGCCAATTCGTTA TCGCTCAGGCGAACGCTAAGCTAAACGAAGATGGTACTTTTGCA GATGAGCTGATCACAGCTCGTCAGAAAGGTGAATCTGGTCTACA CCCTCGTGAGCACGCTCAGTACATGGACGTTGCGACAAACCAGG TTGTATCTATCGCTGCATCGCTTATCCCGTTCCTAGAACACGATG ATGCGAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCA GTTCCAA A3.3 TGATGCGCCCTGGTGAGCCACCAACAAAAGAAGCTGCAGAAGC ACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTTACGATCTATC GACTGTTGGTCGTATGAAGTTTAATAGCTCTATCGGTCGTGAAG ATGCTCAAGAGCAAGGCACACTTGATGAAACAGATATCATCGAA GTGATGAAGAAGCTTATCGCGATCCGTAACGGCAAAGGCGAAGT GGACGATATCGACCACCTAGGCAACCGTCGTATCCGTTCTGTCGG 154 CGAAATGGCTGAGAACCAGTTCCGTGTAGGTCTAGTTCGTGTTGA GCGTGCAGTTAAAGAGCGTCTGAGTCTGGGCGACCTTGATGCGAT CATGCCTCAAGACCTGATCAATGCGAAACCAATTTCTGCAGCGGT TAAAGAATTCTTTGGCTCTTCACAGCTTTCACAGTTCATGGACCA AAACAACCCGTTATCAGAAGTAACGCACAAGCGTCGTATTTCTGC ATTGGGTCCTGGCGGTCTAACTCGTGAACGTGCTGGCTTCGAAGT TCGAGACGTACACGTAACTCACTACGGCCGTCTATGTCCTATCGA AACGCCGGAAGGTCCAAACATCGGTCTGATCAACTCTCTATCTGC GTTTGCGCGTTGTAACGAGTACGGTTTCCTTGAGACGCCATACCG TCGCGTTGTAGATGGTGTTGTAACAGACGAAGTAGATTACCTATC TGCAATCGAAGAAGGCCAATTCGTTATCGCTCAGGCGAACGCTA AGCTAAACGAAGATGGTACTTTTGCAGATGAGCTGATCACAGCTC GTCAGAAAGGTGAATCTGGTCTACATCCTCGTGAGCACGCTCAGT ACATGGACGTTGCGACAAACCAGGTTGTATCTATCGCTGCATCGC TTATCCCGTTCCTAGAACACGATGATGCGAACC A650 TGATGCGCCCTGGTGAGCCACCAACAAAAGAAGCTGCAGAAGC ACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTTACGATCTATC GACTGTTGGTCGTATGAAGTTTAATAGCTCTATCGGTCGTGAAG ATGCTCAAGAGCAAGGCACACTTGATGAAACAGATATCATCGAA GTGATGAAGAAGCTTATCGCGATCCGTAACGGCAAAGGCGAAG TGGACGATATCGACCACCTAGGCAACCGTCGTATCCGTTCTGTC GGCGAAATGGCTGAGAACCAGTTCCGTGTAGGTCTAGTACGTGT TGAGCGTGCAGTTAAAGAGCGTCTAAGCCTAGGCGATCTTGACG CAATCATGCCTCAAGACCTGATCAACGCGAAGCCAATTTCTGCA GCGGTTAAAGAATTCTTTGGCTCTTCACAGCTTTCACCGTTCATG GACCAAAACAACCCGCTGTCAGAAGTAACGCACAAGCGTCGTAT TTCTGCATTGGGTCCTGGCGGTCTGACTCGTGAACGTGCTGGCTT CGAAGTTCGAGACGTACACGTAACTCACTACGGTCGTCTATGTC CTATCGAAACGCCGGAAGGTCCAAACATCGGTCTGATCAACTCT CTATCTGCGTTTGCGCGTTGTAACGAGTACGGTTTCCTTGAGACG CCATACCGTCGCGTTGTAGATGGTGTTGTAACAGACGAAGTAGA TTACCTATCTGCAATCGAAGAAGGCCAATTCGTTATCGCTCAGG CGAACGCTAAGCTAAACGAAGATGGTACTTTTGCAGATGAGCTG ATCACAGCTCGTCAGAAAGGTGAATCTGGTCTACACCCTCGTGA GCACGCTCAGTACATGGACGTTGCGACAAACCAGGTTGTATCTA TCGCTGCATCGCTTATCCCGTTCCTAGAACACGATGATGCGAAC C 155 Phụ lục 5: Trình tự chuỗi polypeptide suy diễn từ đoạn gen rpoB Chủng/ dòng Trình tự chuỗi polypeptide suy diễn từ đoạn gen rpoB LBT6 PGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQEQG TLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAENQFR VGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSSQLS QFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHYGR LCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTDEVD YLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPREHA QYMDVATNQVVSIAASLIPFLEHDDANRALMGANMHR L4650 LFENLFFSEDRYDLSAVGRMKFNSSIGRDDAEEQGTLDKEDIIEVMK KLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVK ERLSLGDLDAIMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSE ITHKRRISALGPGGLTRERAGFEVRDVHVTHYGRVCPIETPEGPNIG LINSLSVYARCNEYGFLETPYRRVVDGVVTDEVHYLSAIEEGQYVI AQANTNLDEEGTFADDLVTARQKGESSLLPRDQVQYMDVSTNQV VSVGASLIPFLEHDDAN A3.3 MRPGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQE QGTLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAEN QFRVGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSS QLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTH YGRLCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTD EVDYLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPRE HAQYMDVATNQVVSIAASLIPFLEHDDAN A650 MRPGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQE QGTLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAEN QFRVGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSS QLSPFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHY GRLCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTDE VDYLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPRE HAQYMDVATNQVVSIAASLIPFLEHDDAN 156 Phụ lục 6: So sánh trình tự gen toxR của chủng V. parahaemolyticus A3.3 và B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus CDC_K4557 1618 100 0,0 99,89 CP006008.1 2 V. parahaemolyticus BB22OP 1618 100 0,0 99,89 CP003972.1 3 V. parahaemolyticus FORC_072 1591 100 0,0 99,32 CP013472.1 4 V. parahaemolyticus R13 1591 100 0,0 99,32 CP028342.1 5 V. parahaemolyticus R14 1591 100 0,0 99,32 CP028341.1 6 V. parahaemolyticus toxR and toxS genes 1591 100 0,0 99,32 L11929.1 7 V. parahaemolyticus MVP1 1585 100 0,0 99,20 CP043421.1 8 V. parahaemolyticus ATCC 17802 1585 100 0,0 99,20 AY527396.1 50 V. parahaemolyticus S107-1 1552 100 0,0 98,52 CP028481.1 100 V. parahaemolyticus Vp2010-008 toxR 948 60 0,0 98,87 KT194122.1 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus CDC_K4557 1618 100 0,0 99,89 CP006008.1 2 V. parahaemolyticus BB22OP 1618 100 0,0 99,89 CP003972.1 3 V. parahaemolyticus FORC_072 1591 100 0,0 99,32 CP013472.1 4 V. parahaemolyticus R13 1591 100 0,0 99,32 CP028342.1 5 V. parahaemolyticus R14 1591 100 0,0 99,32 CP028341.1 6 V. parahaemolyticus toxR and toxS genes 1591 100 0,0 99,32 L11929.1 7 V. parahaemolyticus MVP1 1585 100 0,0 99,20 CP043421.1 8 V. parahaemolyticus ATCC 17802 1585 100 0,0 99,20 AY527396.1 157 50 V. parahaemolyticus FORC-004 1552 100 0,0 98,52 CP028481.1 100 V. parahaemolyticus Vp2010-068 toxR 948 60 0,0 98,87 KT194123.1 Phụ lục 7: So sánh trình tự gen tlh của chủng V. parahaemolyticus A3.3, B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FORC_006 2300 100 0,0 99,76 CP009766.1 2 V. parahaemolyticus FORC-023 CP012951.1 3 V. parahaemolyticus 160807 2278 100 0,0 99,44 CP033142.1 4 V. parahaemolyticus FORC-071 2272 100 0,0 99,36 CP023486.1 5 V. parahaemolyticus UCM-V493 2266 100 0,0 99,28 CP007005.1 6 V. parahaemolyticus MVP1 2255 100 0,0 99,12 CP043422.1 7 V. parahaemolyticus S107-1 2255 100 0,0 99,12 CP028482.1 8 V. parahaemolyticus VH12 2255 100 0,0 99,12 KT948112.1 9 V. parahaemolyticus Vp-18 2255 100 0,0 99,12 JF513048.1 10 V. parahaemolyticus Vb0624 2250 100 0,0 99,04 CP041201.1 44 V. parahaemolyticus 1682 1552 100 0,0 89,00 CP012095.1 97 V. parahaemolyticus TBW-9-11-1 802 35 0,0 98,89 JX262952.1 100 V. harveyi VIB647 800 92 0,0 79,13 DQ224369.1 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FORC_006 2300 100 0,0 99,76 CP009766.1 2 V. parahaemolyticus FORC-023 CP012951.1 158 3 V. parahaemolyticus 160807 2278 100 0,0 99,44 CP033142.1 4 V. parahaemolyticus FORC-071 2272 100 0,0 99,36 CP023486.1 5 V. parahaemolyticus UCM-V493 2266 100 0,0 99,28 CP007005.1 6 V. parahaemolyticus MVP1 2261 100 0,0 99,20 CP031782.1 7 V. parahaemolyticus VPD14 2261 100 0,0 99,20 CP028482.1 8 V. parahaemolyticus 20130629002S01 2261 100 0,0 99,20 CP028482.1 42 V. parahaemolyticus VP1.1997 2135 94 0,0 99,16 KT948114.1 97 V. parahaemolyticus TBW-9-11-1 808 35 0,0 99,11 JX262952.1 100 V. diabolicusi JPW-8-11-8 802 35 0,0 98,89 JX262986.1 Phụ lục 8: So sánh trình tự gen rpoB của chủng V. parahaemolyticus A3.3, B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FDAARGOS_51 1701 100 0,0 99,78 CP026041.1 2 V. parahaemolyticus MAVP-26 1701 100 0,0 99,78 CP0232248.1 3 V. parahaemolyticus CHN25 1690 100 0,0 99,57 CP010883.1 4 V. parahaemolyticus HA2 1685 100 0,0 99,46 CP023710.1 5 V. parahaemolyticus VN-10092 1657 100 0,0 98,92 KJ647551.1 6 V. parahaemolyticus ATCC 17802 1688 99 0,0 98,64 CP014046.1 7 V. parahaemolyticus BB22OP 1640 100 0,0 98,60 CP003972.1 50 V. parahaemolyticus VN-2815 1629 100 0,0 98,38 KJ647561.1 100 V. parahaemolyticus VN-4027 1616 100 0,0 98,06 KJ648147.1 159 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus ATCC17802 1792 100 0,0 99,69 CP014046.2 2 V. parahaemolyticus UCM-V493 1792 100 0,0 99,69 CP007004.1 3 V. parahaemolyticus FORC_006 1781 100 0,0 94,49 CP009765.1 4 V. parahaemolyticus VN-3112 1764 98 0,0 99,69 KJ647744.1 5 V. parahaemolyticus 17802 1764 98 0,0 99,69 EU909181.1 6 V. parahaemolyticus VN-4004 1755 98 0,0 98,48 JK647843.1 50 V. parahaemolyticus SO150 1703 100 0,0 98,06 HQ533927.1 100 V. parahaemolyticus VN-4019 1679 98 0,0 98,02 KJ648140.1 Phụ lục 9: Cấu trúc bậc 2 của TLH ở các chủng/dòng vi khuẩn Email bichhuyenbh88@gmail.com Description LBT6-TLH_ Date Thu Aug 22 08:52:38 BST2019 Unique Job ID c435cab434f11aee Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EY R F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 22%) Alpha helix ( 31%) Beta strand ( 15%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description L4650-TLH Date Sun Aug 25 03:50:06 BST2019 Unique Job ID 0e1e7c751d2b9f96 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I P L L T A L L P L A S A V A E K P T L S P E MV S A S EV I S T Q E N Q T Y T Y V R C WY R TS YSK D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T NT S Q N V I R Q R C E A T L D L A N EN ADI T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I I K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWFL G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KPA N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D AG A K N F ML MT V P D V T Secondary structure SS confidence Disorder ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 25%) Alpha helix ( 26%) Beta strand ( 23%) Email bichhuyenbh88@gmail.com Description L4200-TLH+39bp Date Sat Aug 24 13:14:17 BST2019 Unique Job ID e0dff388e84cf5bf Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . . . 420 Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N K L A AV P F I T S Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 430 . Sequence H T R K T E R H A F V Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 23%) Alpha helix ( 31%) Beta strand ( 14%) Email bichhuyenbh88@gmail.com Description L3900-TLH Date Thu Aug 22 09:35:04 BST2019 Unique Job ID 85de98dc21e5ba0b Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L F P V A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EY R F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 20%) Alpha helix ( 31%) Beta strand ( 15%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description B3_13-TLH Date Thu Aug 22 09:54:03 BST2019 Unique Job ID 5fd3a982e0312ab6 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EY R F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 22%) Alpha helix ( 31%) Beta strand ( 15%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description B7050-TLH Date Thu Aug 22 09:58:42 BST2019 Unique Job ID cf48b13aeac5c243 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YSK D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N ML Y T N T S Q N V I R Q R C E A T L D L A N EN ADI T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KPA N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G F P E V K A E Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YST Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DPC L D I N Secondary structure SS confidence Disorder ? ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EYR F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 22%) Alpha helix ( 32%) Beta strand ( 16%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description B7900-TLH Date Thu Aug 22 10:00:53 BST2019 Unique Job ID e98ea2433cbc140d Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G F N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EY R F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 21%) Alpha helix ( 30%) Beta strand ( 16%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description A3_3_____ Date Thu Aug 22 09:43:36 BST2019 Unique Job ID da03514f02b57c04 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YS K D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN AD I T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KP A N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YS T Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E E H G F V N AS DP C L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V D Y MY T H A L R S E C A A S G A E K F V F WD V T H P T T A T H R Y V A E K ML E S S N N L A EY R F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 22%) Alpha helix ( 31%) Beta strand ( 15%) TM helix ( 4%) Email bichhuyenbh88@gmail.com Description A400-LBT6 Date Thu Aug 22 09:48:34 BST2019 Unique Job ID acc7c65ec56be0b0 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YSK D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN ADI T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWF L G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KPA N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YST Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P E D H G F V N AS DPC L D I N Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 . . . . . . . . . 410 . . . . . . . Sequence R S S S V N Y MD T H A L R S E C A A S G A E K F V F WD V T H P T T A T H L Y V A E K ML E S S N N L A EYR F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 20%) Alpha helix ( 33%) Beta strand ( 11%) Email bichhuyenbh88@gmail.com Description A650-TLH_ Date Sun Aug 25 11:14:44 BST2019 Unique Job ID 3982b204bd9f33a3 Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence MK K T I T L L T A L L P L A S A V A E E P T L S P E MV S A S E V I S T Q E N Q T Y T Y V R C WY R TS YSK D D P A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence T D WE WA K N E D G S Y F T I D G Y WWS S V S F K N MF Y T N T S Q N V I R Q R C E A T L D L A N EN ADI T F F A Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence A D N R F S Y N H T I WS N D A A MQ P D Q I N K V V A L G D S L S D T G N I F N A S Q WR F P N P N SWFL G H F S N Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence G F V WT E Y I A K A K N L P L Y N WA V G G A A G E N Q Y I A L T G V G E Q V S S Y L T Y A K L A K NY KPA N T L F Secondary structure SS confidence Disorder ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence T L E F G L N D F MN Y N R G V P E V K A D Y A E A L I R L T D A G A K N F ML MT L P D A T K A P Q F K YST Q E E I Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . Sequence D K I R A K V L E MN E F I K A Q A MY Y K A Q G Y N I T L F D T H A L F E T L T S A P K N T V S Secondary structure SS confidence Disorder ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 22%) Alpha helix ( 31%) Beta strand ( 18%) Email bichhuyenbh88@gmail.com Description VvPlpA___ Date Tue Sep 17 15:30:57 BST2019 Unique Job ID c356ec62c1e2da4b Secondary structure and disorder prediction 1 . . . . . . . . 10 . . . . . . . . . 20 . . . . . . . . . 30 . . . . . . . . . 40 . . . . . . . . . 50 . . . . . . . . . 60 Sequence V A D E P A L S P E A I T S A Q V F S T Q S K E T Y T Y V R C WY R T G NSHD ES A T D WE WA E N P D G S Y F T I D Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 70 . . . . . . . . . 80 . . . . . . . . . 90 . . . . . . . . . 100 . . . . . . . . . 110 . . . . . . . . . 120 Sequence G Y WWS S V R L K N MF Y T N T S Q N V I K Q R C E E T L G V T H D A ADI T YF A A D N R WS Y N H T I WT N D P V Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 130 . . . . . . . . . 140 . . . . . . . . . 150 . . . . . . . . . 160 . . . . . . . . . 170 . . . . . . . . . 180 Sequence MQ A D Q I N K I V A F G D S L S D T G N I F N A A Q WR F P N P D T WF L GH F S N G F V WT E Y I A Q A K K L P L Y Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? Disorder confidence . . . . . . . . . 190 . . . . . . . . . 200 . . . . . . . . . 210 . . . . . . . . . 220 . . . . . . . . . 230 . . . . . . . . . 240 Sequence N WA V G G A A G S N Q Y V A L T G V K D Q V L S Y L T Y A K MA K N Y KPEN TL F T L E F G L N D F MN Y N R E V V Secondary structure SS confidence Disorder ? ? ? ? Disorder confidence . . . . . . . . . 250 . . . . . . . . . 260 . . . . . . . . . 270 . . . . . . . . . 280 . . . . . . . . . 290 . . . . . . . . . 300 Sequence D V K T D F S T A L I K L T D A G A K N I ML MT L P D A T K A P Q F K YSTQAE I E K V R A K I V E F N E F I K A Q Secondary structure SS confidence Disorder ? ? ? ? Disorder confidence . . . . . . . . . 310 . . . . . . . . . 320 . . . . . . . . . 330 . . . . . . . . . 340 . . . . . . . . . 350 . . . . . . . . . 360 Sequence A A F Y I I Q G Y N I T L Y D T H G L F E Q L T Q N P Q Q H G F V N A S DACL NI N R A S S A D Y L Y S H S L T N E C Secondary structure SS confidence Disorder ? Disorder confidence . . . . . . . . . 370 . . . . . . . . . 380 . . . . . . . . . 390 . . . . . . . . . 400 Sequence A T H S S D K Y V F WD V T H P T T A V H K Y I A E K ML A P G A G MQ RF NF Secondary structure SS confidence Disorder ? ? ? ? ? ? ? ? ? Disorder confidence Confidence Key High(9) Low (0) ? Disordered ( 21%) Alpha helix ( 33%) Beta strand ( 12%)