Luận án Đánh giá hoạt động của một số gen liên quan đến tổng hợp, tích lũy tinh bột và thử nghiệm chuyển gen SSIV vào cây sắn

Vàng 95 KM305-1 23.5 35.9 27 323 Thẳng, nhánh vừa 245 Trắng 96 KM308-2 23.5 35.9 32.75 314 Thẳng, nhánh vừa 240 Trắng 97 KM315-5 23.5 35.9 30 257 Thẳng, nhánh vừa 328 Trắng 98 KM299-1 23.7 36.05 24.75 367 Thẳng, nhánh cao 240 Vàng 99 KM301-9 23.7 36.05 26.75 307 Thẳng, nhánh vừa 249 Vàng 100 KM305-2 23.7 36.05 31.5 336 Thẳng, nhánh vừa 240 Trắng 101 KM297-5 23.8 36.13 17.25 312 Thẳng, nhánh vừa 245 Trắng 102 KM63-2 23.8 36.13 20.75 404 Thẳng, nhánh vừa 248 Trắng 103 Hòa Mãn 911 24 36.7 40 284 104 KM288 24 36.28 34 334 Thẳng, nhánh cao 210 Trắng 105 KM306-4 24 36.28 26.5 323 Thẳng đẹp,0 nhánh 240 Vàng 106 KM318-2 24 36.28 24.5 354 Cao, nhánh vừa 316 Trắng 107 KM98-1 24 36.28 21.25 282 Thẳng, nhánh cao 254 Vàng 108 KM98-1 24 36.28 37.5 292 Thẳng, nhánh thấp 230 Trắng 109 KM98-5 24 36.28 24,5 308 Thẳng, nhánh thấp 245 Trắng 110 DT1 24.1 37.8 40 377.5 154 111 GM 444-2 24.1 38.6 42 305 112 KM293 24.2 36.43 47 320 Thẳng, 0 nhánh 240 Trắng 113 KM298-2 24.2 36.43 33 298 Thẳng, nhánh vừa 274 Vàng 114 KM304-6 24.2 36.43 21.75 313 Thẳng, 0 nhánh 225 Vàng 115 KM309-6 24.2 36.43 46 303 Thẳng, nhánh cao 373 Trắng 116 KM315-3 24.2 36.43 0 423 Cao, 0 nhánh 280 Vàng 117 KM43 24.2 36.43 19 254 Thẳng, nhánh ít 310 Trắng 118 KM228 24.3 36.5 33.5 259 Thẳng, 0 nhánh 240 Trắng 119 KM229 24.3 36.5 32.75 315 Thẳng, 0 nhánh 240 Trắng 120 KM300-2 24.3 36.5 11.5 343 Hơi cong, nhánh 240 Vàng đậm 121 KM301-2 24.3 36.5 10.25 343 Thẳng, 0 nhánh 240 Trắng 122 KM307-2 24.3 36.5 23.75 342 Thẳng, 0 nhánh 240 Trắng 123 KM312-3 24.4 36.58 21 275 Nhánh nhiều 240 Trắng 124 KM316-1 24.4 36.58 37 394 Nhánh, xấu, đổ 316 Trắng 125 Sắn xanh 24.4 38.3 18.4 355 126 KM29 24.5 36.65 28.5 277 Thẳng, 0 nhánh 258 Vàng 127 KM294-4 24.5 36.65 22 429 Thẳng, nhánh vừa 235 Trắng 128 KM301-8 24.5 36.65 26.5 328 Thẳng, 0 nhánh 312 Vàng 129 KM308-4 24.5 36.65 23 320 Thẳng, 0 nhánh 250 Vàng 130 KM309-10 24.5 36.65 18.5 276 Thẳng, nhánh vừa 254 Trắng 131 KM310-6 24.5 36.65 38 276 Thẳng đẹp,0 nhánh 268 Trắng 132 KM312-4 24.5 36.65 20 313 Thẳng, 0 nhánh 265 Vàng 133 ORM 35.8 24.5 39 30 220 134 Thân đen 24.5 38.6 20 295 135 KM 35-1 24.6 36.73 25 256 Thấp, nhánh, xấu 248 Trắng 136 KM273-2 24.6 36.73 29 445 Đổ ngã, nhánh 249 Trắng 137 KM14-1 24.8 36.88 43 284 Thẳng, nhánh cao 264 Trắng 138 KM300-1 24.9 36.95 52.5 415 Hơi cong, nhánh 240 Vàng 139 HB 60 25 37.03 30.5 251 Thẳng. nhánh cao 254 Trắng 155 140 Hòa Mãn 125 25 37.4 28 225 141 KM302-13 25 37.03 18 269 Nhánh, xấu 245 Vàng 142 KM7 25 37.03 28 320 Thẳng, nhánh vừa 265 Vàng 143 LC 1 25.1 37 32 265 144 Chuối vàng 25.2 38.8 8.8 365 145 Nêu Co 25.2 36.9 20 335 146 Sắn trắng 25.2 36.9 20 337.5 147 Sắn trắng MN 25.2 36.9 7 367.5 148 DT3 25.3 39.6 18 214 149 HG 2 25.3 37.5 24 317.5 150 Rayong 5 25.3 37.25 35.7 356 Thẳng, nhánh cao 240 Trắng 151 KM297-4 25.4 37.33 28 281 Thẳng, nhánh ít 273 Vàng 152 KM308-10 25.4 37.33 15.25 258 Thẳng, nhánh vừa 265 Vàng 153 KM314-6 25.4 37.33 25.5 368 Thẳng, nhánh cao 310 Trắng 154 KM315-4 25.4 37.33 35.5 358 Cao, nhánh vừa 315 Trắng 155 KM5 25.4 37.33 5.5 349 Nhánh nhiều 272 Trắng 156 KM111-1 25.5 37.4 31.5 276 Thẳng, nhánh cao 248 Trắng 157 KM146 25.5 37.4 45 246 Thẳng, 0 nhánh 248 Trắng 158 Bún trắng 25.6 36.3 22.5 320 159 KM108 25.7 37.55 31.5 332 Thẳng, 0 nhánh 280 Vàng 160 KM264 25.7 37.55 25 313 Thẳng, nhánh vừa 260 Trắng 161 KM79 25.7 37.55 37 393 Thẳng, 0 nhánh 335 Trắng 162 Sắn đỏ Nghệ An 25.7 37.3 18.4 341.5 163 KM313-2 25.9 37.7 24.5 325 Thẳng, 0 nhánh 316 Trắng 164 KM318-9 26 37.78 35 367 Thẳng, nhánh vừa 311 Vàng 165 KM 21-10 26.1 39.1 24 340 297 166 KM94 26.1 37.85 53.5 289 Cong, nhánh ở gốc 330 Trắng 167 SC 205 26.1 37.85 25.7 340 Thẳng, nhánh cao 311 Trắng 156 168 KM294-3 26.2 37.93 33.25 307 Thấp xấu, nhánh 220 Trắng 169 KM302-8 26.2 37.93 32 307 Thẳng, đẹp 240 Trắng 170 KM305-3 26.2 37.93 23 326 Thẳng, 0 nhánh 230 Trắng 171 KM313-7 26.2 37.93 28.75 386 Cao, nhánh, đổ 300 Trắng 172 KM314-8 26.2 37.93 42.5 341 Thẳng, nhánh cao 305 Trắng 173 HL03 26.3 38 24 280 Cong, nhánh ít 267 Trắng 174 Sắn nếp 26.3 37.2 14 330 175 KM285 26.4 38.08 16.75 370 Thẳng, 0 nhánh 235 Trắng 176 KM68 26.4 38.08 22 173 Nhánh, cây xấu 256 Trắng 177 KM948-5 26.4 37,65 15.5 267 Thẳng, 0 nhánh 318 Trắng 178 RAY ONG 2 26.4 36,2 24,0 225 179 SC 205 26.4 37.2 33.6 247.5 180 GM 155-7 26.5 39.6 32 292 181 KM05-1 26.5 38.15 30 429 Thẳng, nhánh vừa 235 Trắng 182 KM309-2 26.5 38.15 16 278 Thẳng, nhánh vừa 268 Vàng 183 KM315-1 26.5 38.15 51.5 387 Thẳng, nhánh cao 284 Trắng 184 KM42 26.5 38.15 42.5 229 Thẳng, nhánh cao 311 Trắng 185 TB-CCC-8 Sắn cần câu 26.7 36.7 26.7 337.5 186 KM289-3 26.9 38.45 19.25 275 Thẳng, nhánh vừa 245 Trắng 187 KM312-5 26.9 38.45 41 297 Xấu, nhánh 260 Trắng 188 KM73 26.9 38.45 14.5 416 Thẳng, 0 nhánh 318 Trắng 189 Sắn trắng 26.9 36.7 27.6 285 190 Sắn đổ 26.9 36.7 3.6 285 191 Chuối đỏ 27 38.6 22.5 345 192 HB 80 27 38.53 26.5 246 Thẳng, 0 nhánh 248 Trắng 193 KM140 27 35.97 50.5 285 Thẳng, 0 nhánh 240 Trắng 194 KM314-4 27 38.53 29 329 Thẳng cao, 0 nhánh 316 Trắng 195 KM318-5 27 38.53 35.5 388 Cong, nhánh vừa 312 Vàng 196 KM318-7 27 38.53 23.5 267 Thẳng, nhánh vừa 312 Trắng 157 197 Xanh Hà Bắc 27 37.5 20,0 193.5 198 Xanh Vĩnh phú 27 38.6 27.5 331.5 Thẳng, nhánh cao 264 Trắng 199 KM302-1 27.2 38.68 28.75 284 Thẳng, 0 nhánh 312 Vàng 200 KM311-2 27.2 38.68 13 250 Nhánh nhiều 270 Vàng 201 KM75 27.2 38.68 15 410 Thẳng, 0 nhánh 312 Trắng 202 HG 1 27.3 38.3 27.6 329 203 HB 80 27.3 38.8 28 220 300 204 Rayong 9 27.3 38.75 15 267 Thẳng, 0 nhánh 300 Trắng 205 KM308-6 27.4 38.83 30 267 Nhánh, xấu 294 Trắng 206 SM 305 27.4 38.83 35 290 Thẳng, nhánh ít 310 Trắng 207 HL23 27.5 38.08 16.5 283 Nhánh ở gốc, thẳng 210 Trắng 208 DT2 27.6 36.2 17.6 182.5 209 KM 325 27.6 38.6 40 192.5 210 HL 2003-14 27.7 35.5 16.8 320 211 Canh nông 27.8 35.5 18 345 212 Sắn Tuyên Quang 27.8 39.1 8 275 213 Trắng Bắc Thái 27.8 39.7 30 353 214 KM 397 27.9 38.4 26.8 185 215 Chuối vàng 28 38 18.8 370 216 GON 28 35.6 17.8 320 Phân cành cao 315 Trắng 217 KM98-5 28 35.67 37.5 316 Thẳng, nhánh cao 248 Trắng 218 SA 06 28.1 39.6 40 321 Thẳng, 0 nhánh 255 Trắng 219 Sắn đỏ 28.2 40,0 18,0 283.5 220 TB-CCC-4 28.2 39.5 41 340 221 KM98-7 28.3 39.5 38.6 405 Nhánh nhiều 310 Trắng 222 KM 98-7 28.32 37.6 34 382.5 264 223 CM40.18.3 28.4 40.8 24 255 224 TB-CCC-2 Sắn nếp 28.4 39.5 31 298.5 158 225 KM313-4 28.5 39.65 29 358 Thẳng, 0 nhánh 321 Trắng 226 KM316-4 28.5 39.65 23 336 Nhánh ít, đổ 310 Trắng 227 KM318-8 28.5 39.65 21 349 Nhánh nhiều 310 Trắng 228 SM 232.10 28.5 40.6 36 315 229 HB 60 28.6 36.3 20 195 230 SM 25956.27 28.6 39.5 14.8 255 231 KM310-3 28.7 39.8 30.25 306 Thẳng, 0 nhánh 275 Trắng 232 OMR 39.43.27 28.7 40,1 28,8 263 233 Sắn đỏ miền nam 28.7 39.7 30.8 335 234 KM 140 28.9 39.5 20 231 235 SM 2938-20 28.9 40.8 24 315 236 RAY ONG 3 29 37.5 20.8 227 237 Sắn học viện 29 38 16.4 345 238 SM 2554.10 29 40.1 22.5 262.5 239 KM505 29.1 40.11 10.75 356 Nhánh nhiều 376 Trắng 240 KM 104-2 29.2 40.6 16.4 365 241 SM 1868.1 29.3 40.8 18 283.5 242 Sắn đỏ ĐF 29.5 41.1 13 281.5 Thẳng, 0 nhánh 255 Đỏ 243 SM937-26 29.5 40.41 45 283 Thẳng, 0 nhánh 252 Trắng 244 BT (60) 29.6 39.2 20 305 245 HL 2002-120 29.7 40.7 22 340 246 KM 108-2 29.7 40.2 22 372.5 289 247 SM 1561-2 29.7 37.5 19.6 282.5 248 KM 21-12 29.8 35.5 13 385 305 249 SM 2956.34 29.9 40.5 13.3 232.5 250 KM 94 27,6 38.3 30.4 335 159 PHỤ LỤC 2 Thông tin về các trình tự nucleotide của các gen/đoạn gen phân lập và đăng ký 1. Starch synthase IV (SSIV) LOCUS KT033500 3224 bp mRNA linear PLN 18-NOV-2015 DEFINITION Manihot esculenta starch synthase IV (SSIV) mRNA, complete cds. ACCESSION KT033500 VERSION KT033500.1 GI:952183002 KEYWORDS . SOURCE Manihot esculenta (cassava) ORGANISM Manihot esculenta Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae; Manihot. REFERENCE 1 (bases 1 to 3224) AUTHORS Le,N.T., Nguyen,D.V., Nguyen,H.M., Nguyen,H.T.M., Pham,N.B. and Chu,H.H. TITLE Isolation and overexpression of cassava-derived starch synthase class IV for enhanced starch production in plant JOURNAL Unpublished REFERENCE 2 (bases 1 to 3224) AUTHORS Le,N.T., Nguyen,D.V., Nguyen,H.M., Nguyen,H.T.M., Pham,N.B. and Chu,H.H. TITLE Direct Submission JOURNAL Submitted (08-JUN-2015) Plant Cell Biotechnology, Institute of Biotechnology, 18 Hoang Quoc Viet, Hanoi 1000, Vietnam FEATURES Location/Qualifiers source 1..3224 /organism="Manihot esculenta" /mol_type="mRNA" /db_xref="taxon:3983" 14gene 1..3224 /gene="SSIV" /note="SSIV" 14CDS 1..3186 /gene="SSIV" /codon_start=1 /product="starch synthase IV" /protein_id="ALN98281.1" /db_xref="GI:952183003" /translation="MASKLSTWFLSQGFTALNYNFDTNKQTATRFLLPSHRLLPASCK MRQRNLSSQHKRQQLKKASPEQPPNTVGFHSSGGGGGDDDIGDDDNDSETDSTAVHSV PSLNLDVESNEEVVDVSVDVEHAQHTGANDVERNVDMEHVQDVGAKDLYSLTQEMKTL GIDGAEKLSSIPDEMKPLVLNKDGGEQLSSFQLEDLIGMIRNAEKNILLLNQARVHAL EDLERILAEKEILQGEINVLEMKLAGTDARMKVAAQEKMHVELMEDQLGKLRNELAYR VGNQNKLLNEEAPLIQDSTIQNISEELNSLRAENTSLRTDIEALKRELSNVKDTDERV ITLEKECMQLESSVKDLESKLSVSQEDVSKLSSLKVECKDLWEKVGSLQALLDKATKQ ADQAILVLQQNRDLWKKVDKLEESLEEANIYKLSSEKLQQYNELMQQKIKLLEERLQR SDEEIYSYVQLYQESIQEFQDTLNTLKEESKKKALDEPVDDMPWQFWSHLLLMIDGWL LEKKLTLDDAKLLRDMVWKRERRIHDIYLECREKNEHEAVSMFLKLTSSPKSQGLYVV HIAAEMAPVAKVGGLGDVVTGLGKALQKRGHLVEIILPKYDCMQYDGIGNLRALDVVL ESYFDGKLYKNEVWVGTIEGLPVYFIEPHHPGKFFWRGQFYGEHDDFKRFSFFSRAAL ELLLQAGKKPDIIHCHDWQTAFVAPLYWDIYAPKGLNSARICFTCHNFEYQGSAPASE LASCGLDVQQLNRPDRMQDNSAHDRINPIKGAVVFSNIVTTVSPTYAQEVRTSEGGKG LHSTLNFHAKKFIGILNGIDTDVWNPATDTLLEVQYNANDLQGKAENKIATRQHLGLS TADARQPLVGCITRLVPQKGVHLIRHAIYRTLELGGQFLLLGSSPVAHIQREFEGIAN HFQNHEHIRLVLKYDESLAHSIYAASDMFIIPSIFEPCGLTQMIAMRYGSIPIARKTG GLNDSVLDVDDDTIPLQFRNGYTFLNPDEQGVNSALERAFNHYRNDPESWQQLVQKDM NIDFSWESSASQYEELYSKSVARARAAASRS" ORIGIN 160 1 atggcgtcga agctatcgac gtggtttctg agtcaagggt tcacagcttt gaactataat 61 tttgacacta ataagcagac agctacgcga ttcttattgc cttcccatcg attgcttcct 121 gcttcttgca aaatgcgaca gcgcaatttg agctctcagc ataagagaca gcagctcaag 181 aaagcctctc ctgaacaacc tccaaatacc gtaggttttc attcgagtgg tggtggtggt 241 ggtgatgatg atattggtga tgatgataat gattccgaaa ctgatagcac agcggtgcat 301 agtgtcccaa gcttgaatct tgatgttgag agtaatgagg aagtcgtcga tgttagcgta 361 gatgtggagc atgctcagca tacgggtgca aatgatgtgg agaggaatgt agatatggaa 421 catgttcaag atgttggtgc aaaagatttg tatagtctca cccaggaaat gaaaactttg 481 ggcatagatg gagcagagaa gctttctagt attcctgatg aaatgaaacc tttggtctta 541 aacaaagatg gtggagaaca actgtcaagc tttcaacttg aggatttgat aggcatgata 601 agaaatgctg agaaaaatat cctgcttctc aatcaagctc gggttcatgc acttgaagat 661 cttgaaagaa ttcttgcaga gaaggaaata ttacaaggag aaattaacgt tttagagatg 721 aaattggcag gaactgatgc aagaatgaaa gttgctgctc aagaaaagat gcatgtagaa 781 ctcatggaag accaattagg aaaactaaga aacgaactgg cttacagggt tgggaatcag 841 aacaaacttt tgaatgagga agcccccttg attcaggaca gcactattca gaacattagt 901 gaggagctca attcattgag ggcagagaat acatctctga ggactgatat agaagcactt 961 aagagggagc ttagtaatgt caaggataca gatgagcgtg ttataacact ggagaaagaa 1021 tgcatgcagt tggagtcttc tgtgaaagac ttggaatcca aactgtcagt ttctcaggaa 1081 gatgtttcaa aattgtctag cttgaaagtt gaatgtaaag acttgtggga aaaggtggga 1141 agtttgcaag cattgttaga taaggcaaca aagcaagcag atcaggctat tctagtgttg 1201 cagcaaaatc gagatctttg gaagaaggtt gataaattgg aagaatcctt ggaagaggcc 1261 aacatctata agttatcgtc agaaaagtta cagcagtata atgagctaat gcagcaaaag 1321 ataaaactat tggaggaacg tcttcaacga tctgatgaag aaatatattc ttatgttcag 1381 ttataccaag aatctataca ggaatttcaa gatacactta atactttgaa agaagaaagc 1441 aagaaaaagg cactagatga acctgtagat gatatgccct ggcaattttg gagtcattta 1501 ttgcttatga ttgatggttg gcttcttgag aagaagctaa cactagatga tgcaaaactg 1561 ttgagagata tggtatggaa aagagagagg cgtattcatg acatatactt agagtgcagg 1621 gaaaagaatg agcatgaagc tgtttctatg tttctcaagc tgacatcatc accaaaaagt 1681 caaggattat atgtcgtcca tattgcagcg gagatggcac cagttgctaa ggttggtggc 1741 ttgggagatg ttgtgaccgg tcttggaaaa gcactccaaa agagaggaca tcttgtggaa 1801 attattctgc caaagtatga ctgcatgcaa tatgatggta ttggcaattt aagggcccta 1861 gatgtggtgt tggaatctta ttttgatgga aaattataca aaaacgaagt atgggttggc 1921 accattgaag gtcttcctgt ttactttatt gagcctcatc accccggcaa gttcttttgg 1981 agagggcagt tctacggaga acatgatgat ttcaaacgct tttcattttt cagccgtgct 2041 gcacttgaat tgcttcttca agctggcaaa aaaccagaca taattcattg ccatgactgg 2101 cagacagctt ttgttgcacc actttattgg gatatatacg ccccaaaagg attgaattca 2161 gctagaatat gttttacctg tcacaacttt gagtaccagg ggagtgcacc agcatcagaa 2221 ttggcatctt gtggacttga tgtccagcag ctaaacagac cagatagaat gcaggacaac 2281 tcagcacatg ataggatcaa tcctattaag ggtgcagtgg tgttctcaaa cattgtgaca 2341 acagtatcac ccacctatgc acaagaagtg cggacttctg agggcggaaa aggtctccat 2401 tcgacgctta actttcatgc caagaagttc attggaatcc taaatggtat tgatactgat 2461 gtgtggaatc ctgcgactga tactcttctc gaagtccagt acaatgctaa cgatcttcaa 2521 ggaaaagcag aaaacaaaat agctacaagg cagcatcttg ggttatcaac tgcagatgct 2581 aggcagccac tggttggctg cataacaaga ttggtgccac agaaaggtgt acatcttatt 2641 agacatgcaa tataccgtac gctggagttg ggaggacaat ttctacttct tggctcaagc 2701 ccagttgcac atatacagag ggaatttgag ggtattgcaa atcactttca gaatcatgag 2761 cacattcggc tggtattgaa gtatgatgaa tctctcgctc attccattta tgcagcatct 2821 gacatgttca tcatcccatc tatctttgag ccttgtggcc ttacacagat gatagcaatg 2881 agatatggtt ccatacccat tgcaagaaaa accggtggtc taaatgatag tgttttggat 2941 gttgatgatg acacaattcc tcttcagttt cgaaatggat atacattctt gaatcctgat 3001 gagcagggag tgaatagtgc tttagaacgt gcatttaacc attataggaa cgatcctgag 3061 agctggcagc agcttgttca aaaggacatg aacatagatt ttagttggga atcttcagca 3121 tcacagtatg aggagctcta ctcaaaatca gtggccagag caagagcggc agcaagtagg 3181 tcttaattat ggtaaatggc attttcatgg aaagccaagg atct 2. promoter C54 LOCUS AY217353 1084 bp DNA linear PLN 26-JUL-2016 DEFINITION Manihot esculenta glutamic acid-rich protein (c54) gene, promoter region and partial cds. ACCESSION AY217353 VERSION AY217353.1 KEYWORDS . 161 SOURCE Manihot esculenta (cassava) ORGANISM Manihot esculenta Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae; Manihot. REFERENCE 1 (bases 1 to 1084) AUTHORS Zhang,P., Bohl-Zenger,S., Puonti-Kaerlas,J., Potrykus,I. and Gruissem,W. TITLE Two cassava promoters related to vascular expression and storage root formation JOURNAL Planta 218 (2), 192-203 (2003) PUBMED 13680228 REFERENCE 2 (bases 1 to 1084) AUTHORS Zhang,P., Bohl-Zenger,S., Puonti-Kaerlas,J., Potrykus,I. and Gruissem,W. TITLE Direct Submission JOURNAL Submitted (12-JAN-2003) Institute of Plant Sciences, ETH Zuerich, Universitaetsstrasse 2, Zuerich CH-8092, Switzerland FEATURES Location/Qualifiers source 1..1084 /organism="Manihot esculenta" /mol_type="genomic DNA" /cultivar="MCol1505" /db_xref="taxon:3983" /note="authority: Manihot esculenta Crantz" gene 962..>1084 /gene="c54" regulatory 962..968 /regulatory_class="TATA_box" /gene="c54" /note="promoter p54/1.0" mRNA 1011..>1084 /gene="c54" /product="glutamic acid-rich protein" CDS 1082..>1084 /gene="c54" /note="expressed during secondary growth of the storage root" /codon_start=1 /product="glutamic acid-rich protein" /protein_id="AAP57706.1" /translation="M" ORIGIN 1 ggatccacgg gtgtgggcca actccaccgc ccaagagaaa acctaaaatg gagaaatttg 61 tcaaagcttt ttcaacttta cagactaggc ataggtcctg gggcaaacag aacagagctc 121 aggacgctgc ctccctgctt gcccacctgg aatcattgtt ctccgcttcc tgcttatgtc 181 atattctccg atgatttttt attttttaat tttgcgtttc tctttctttg caggtaaaat 241 aagcccatct caaattcaga tttgggtaat catgcgattt gagaaacatt atagaaaaaa 301 ggtgatcaac tccaattgtg tgataatttt gtagatgtgg caattaaggt cggtttggct 361 tttataagaa attagaatta accaatttca taattatatc attcatgact attgattata 421 atatctgaca aatcctgtaa tgatttttaa cccatttcag tatgatttac tcatcccatt 481 ctttatggct gcttcaatat acagagttca tgacatgtga atctgaattt aaatattaat 541 ttctctgttt aattaataat taataatcca tttcatataa tttttttaaa ttaattaatt 601 aattaaagaa gaaaataaga agctgggaac atgataataa taaaggtcac ctacattacc 661 gactgcacaa agcacataag cacaaaaaac caaaagaaag atgatggctt agctgaaaaa 721 ggccctccat tatttacact tttcgatcaa aaacaaatat catgggtaat ttaaaaagat 162 781 aaagtacagc ttacttattt gaaaaaaaat ttttaattca cnattcttaa aatgttgatn 841 tattcctatt gattttgatt tcagagagat gtgaggtcaa atcccaagat tcctcttcct 901 taggtagagt tcccacctct ctccctctca catgaagctt actggcctct actcctctct 961 ctatataagc cactctcagt taccctctct ctgcaccaaa tacactctaa gctctctctt 1021 cacttcctct ttgcttctca ccatttactt ttagtttcta tttcattttc tcttgctttc 1081 tatg // 3. Trình tự nucleotid và axit amin suy diễn của gen SSIV đã phân lập ở hai giống sắn Đỏ ĐF và KM140 163 10 20 30 40 50 60 70 80 90 100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ATGGCGTCGAAGCTATCGACGTGGTTTCTGAGTCAAGGGTTCACAGCTTTGAACTATAATTTTGACACTAATAAGCAGACAGCTACGCGATTCTTATTGC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 110 120 130 140 150 160 170 180 190 200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CTTCCCATCGATTGCTTCCTGCTTCTTGCAAAATGCGACAGCGCAATTTGAGTAGCTCTCAGCATAAGAGACAGCAGCTCAAGAAAGCCTCTCCTGAACA MeSSIV (Do DF) ...................G................................................................................ MeSSiv (KM140) ..................................................---............................................... 210 220 230 240 250 260 270 280 290 300 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ACCTCCAAATACCGTAGGTTTTCATTCGCGTGGTGGTGGTGGTGGTGATGAT---ATTGGTGATGATGATAATGATTCCGAAACTGAGAGCACAGCGGTG MeSSIV (Do DF) .....................C..............................---..........................G.................. MeSSiv (KM140) ............................A.......................GAT................................T............ 310 320 330 340 350 360 370 380 390 400 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CATAGTGTCCCAAGCTTGAATCTTGATGTTGAGAGTAATGAGGAAGTCGTCGATGTTAGCGTAGATGTGGAGCATGCTCAGCATACGGGTGCAAATGATG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 410 420 430 440 450 460 470 480 490 500 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) TGGAGAGGAATGTAGATATGGAACATGTTCAAGATGTTGGTGCAAAAGATTTGTATAGTCTCACCCAGGAAATGAAAACTTTGGGCATAGATGGAGCAGA MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 510 520 530 540 550 560 570 580 590 600 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) GAAGCTTTCTAGTATTCCTGATGAAATGAAACCTTTGGTCTTAAACAAAGATGGTGGAGAACAACTGTCAAGCTTTCAACTTGAGGATTTGATAGGCATG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 610 620 630 640 650 660 670 680 690 700 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ATAAGAAATGCTGAGAAAAATATCCTGCTTCTCAATCAAGCTCGGGTTCATGCACTTGAAGATCTTGAAAGAATTCTTGCAGAGAAGGAAATATTACAAG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 710 720 730 740 750 760 770 780 790 800 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) GAGAAATTAACGTTTTAGAGATGAAATTGGCAGAAACTGATGCAAGAATGAAAGTTGCTGCTCAAGAAAAGATGCATGTAGAACTCATGGAAGACCAATT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................G.................................................................. 164 810 820 830 840 850 860 870 880 890 900 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGGAAAACTAAGAAACGAACTGGCTTACAGGGTTGGGAATCAGAACAAACTTTTGAATGAGGAAGCCCCCTTGATTCAGGACAGCACTATTCAGAACATT MeSSIV (Do DF) ........................................G........................................................... MeSSiv (KM140) .................................................................................................... 910 920 930 940 950 960 970 980 990 1000 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGTGAGGAGCTCAATTCATTGAGGGCAGAGAATACATCTCTGAGGACTGATATAGAAGCACTTAAGAGGGAGCTTAGTAATGTCAAGGATACAGATGAGC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) GTGTTATAACACTGGAGAAAGAATGCATGCAGTTGGAGTCTTCTGTGAAAGACTTGGAATCCAAACTGTCAGTTTCTCAGGAAGATGTTTCAAAATTGTC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) TAGCTTGAAAGTTGAATGTAAAGACTTGTGGGAAAAGGTGGGAAGTTTGCAAGCATTGTTAGATAAGGCAACAAAGCAAGCAGATCAGGCTATTCTAGTG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) TTGCAGCAAAATCGAGATCTTTGGAAGAAGGTTGATAAATTGGAAGAATCCTTGGAAGAGGCCAACGTCTATAAGTTATCGTCAGAAAAGTTACAGCAGT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) ..................................................................A................................. 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ATAATGAGCTAATGCAGCAAAAGATAAAACTATTGGAGGAACGTCTTCAACAATCTGATGAAGAAATATATTCTTATGTTCAGTTATACCAAGAATCTAT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) ...................................................G................................................ 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ACAGGAATTTCAAGATACACTTAATACTTTGAAAGAAGAAAGCAAGAAAAAGGCACTAGATGAACCTGTAGATGATATGCCCTGGCAATTTTGGAGTCAT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) TTATTGCTTATGATTGATGGTTGGCTTCTTGAGAAGAAGCTAACACTAGATGATGCAAAACTGTTGAGAGATATGGTATGGAAAAGAGAGAGGCGTATTC MeSSIV (Do DF) ........................................................................C........................... MeSSiv (KM140) .................................................................................................... 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 165 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ATGACATATACTTAGAGTGCAAGGAAAAGAATGAGCATGAAGCTGTTTCTATGTTTCTCAAGCTGACATCATCACCAAAAAGTCAAGGATTATATGTCGT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .....................G.............................................................................. 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CCATATTGCAGCGGAGATGGCACCAGTTGCTAAGGTTGGTGGCTTGGGAGATGTTGTGACTGGTCTTGGAAAAGCACTCCAAAAGAGAGGACATCTTGTG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) ............................................................C....................................... 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) GAAATTATTCTGCCAAAGTATGACTGCATGCAATATGATGGTATTGGCAATTTAAGGGCCCTAGATGTGGTGTTGGAATCTTATTTTGATGGAAAATTAT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ACAAAAACGAAGTATGGGTTGGCACCATTGAAGGTCTTCCTGTTTACTTTATTGAGCCTCATCACCCCGGCAAGTTCTTTTGGAGAGGGCAGTTCTACGG MeSSIV (Do DF) .................................................................................C.................. MeSSiv (KM140) .................................................................................................... 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGAACATGATGATTTCAAACGCTTTTCATTTTTCAGCCGTGCTGCACTTGAATTGCTTCTTCAAGCTGGCAAAAAACCAGACATAATTCATTGCCATGAC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) TGGCAGACAGCTTTTGTTGCACCACTTTATTGGGATATATACGCCCCAAAAGGATTGAATTCAGCTAGAATATGTTTTACCTGTCACAACTTTGAGTACC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGGGGAGTGCACCAGCATCAGAATTGGCATCTTGTGGACTTGATGTCCAGCAGCTAAACAGACCAGATAGAATGCAGGACAACTCAGCACATGATAGGAT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CAATCCTATTAAGGGTGCAGTGGTGTTCTCAAACATTGTGACAACAGTATCACCCACCTATGCACAAGAAGTGCGGACTTCTGAGGGCGGAAAAGGTCTC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2410 2420 2430 2440 2450 2460 2470 2480 2490 2500 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| 166 MeSSiv (Manes.15G118600.1) CATTCGACGCTTAACTTTCATGCCAAGAAGTTCATTGGAATCCTAAATGGTATTGATACTGATGTGTGGAATCCTGCGACTGATACTCTTCTCGAAGTCC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2510 2520 2530 2540 2550 2560 2570 2580 2590 2600 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGTACAATGCTAACGATCTTCAAGGAAAAGCAGAAAACAAAATAGCTACAAGGCAGCATCTTGGGTTATCAACTGCAGATGCTAGGCAGCCACTGGTTGG MeSSIV (Do DF) ...................A................................................................................ MeSSiv (KM140) .................................................................................................... 2610 2620 2630 2640 2650 2660 2670 2680 2690 2700 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CTGCATAACAAGATTGGTGCCACAGAAAGGTGTACATCTTATTAGACATGCAATATACCGTACGCTGGAGTTGGGAGGACAATTTCTACTTCTTGGCTCA MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) AGCCCAGTTGCACATATACAGAGGGAATTTGAGGGTATTGCAAATCACTTTCAGAATCATGAGCACATTCGGCTGGTATTGAAGTATGATGAATCTCTCG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2810 2820 2830 2840 2850 2860 2870 2880 2890 2900 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CTCATTCCATTTATGCAGCATCTGACATGTTCATCATCCCATCTATCTTTGAGCCTTGTGGCCTTACACAGATGATAGCAATGAGATATGGTTCCATACC MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 2910 2920 2930 2940 2950 2960 2970 2980 2990 3000 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) CATTGCAAGAAAAACCGGTGGTCTAAATGATAGTGTTTTTGATGTTGATGATGACACAATTCCTCTTCAGTTTCGAAATGGATATACATTCTTGAATCCT MeSSIV (Do DF) ............G....................................................................................... MeSSiv (KM140) .......................................G............................................................ 3010 3020 3030 3040 3050 3060 3070 3080 3090 3100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) GATGAGCAGGGAGTGAATAGTGCTTTAGAACGTGCATTTAACCATTATAGAAACGATCCTGAGAGCTGGCAGCAGCTTGTTCAAAGGGACATGGACATAG MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) ..................................................G..................................A.......A...... 3110 3120 3130 3140 3150 3160 3170 3180 3190 3200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ATTTTAGTTGGGAATCTTCAGCATCACAGTATGAGGAGCTCTACTCAAAATCAGTGGCCAGAGCAAGAGCGGCAGCAAGTAGGTCTTAATTATGGTAAAT MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 3210 3220 ....|....|....|....|....|.. MeSSiv (Manes.15G118600.1) GGCATTTTCATGGAAAGCCAAGGATCT MeSSIV (Do DF) ........................... MeSSiv (KM140) ........................... 167 10 20 30 40 50 60 70 80 90 100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) ASKLSTWFLSQGFTALNYNFDTNKQTATRFLLPSHRLLPASCKMRQRNLSSSQHKRQQLKKASPEQPPNTVGFHSRGGGGGDD-IGDDDNDSETESTAVH MeSSIV (Do DF) ...................................................................................-................ MeSSiv (KM140) .................................................-.........................S.......D..........D..... 110 120 130 140 150 160 170 180 190 200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) SVPSLNLDVESNEEVVDVSVDVEHAQHTGANDVERNVDMEHVQDVGAKDLYSLTQEMKTLGIDGAEKLSSIPDEMKPLVLNKDGGEQLSSFQLEDLIGMI MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 210 220 230 240 250 260 270 280 290 300 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) RNAEKNILLLNQARVHALEDLERILAEKEILQGEINVLEMKLAETDARMKVAAQEKMHVELMEDQLGKLRNELAYRVGNQNKLLNEEAPLIQDSTIQNIS MeSSIV (Do DF) ...............................................................................E.................... MeSSiv (KM140) ...........................................G........................................................ 310 320 330 340 350 360 370 380 390 400 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) EELNSLRAENTSLRTDIEALKRELSNVKDTDERVITLEKECMQLESSVKDLESKLSVSQEDVSKLSSLKVECKDLWEKVGSLQALLDKATKQADQAILVL MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 410 420 430 440 450 460 470 480 490 500 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) QQNRDLWKKVDKLEESLEEANVYKLSSEKLQQYNELMQQKIKLLEERLQQSDEEIYSYVQLYQESIQEFQDTLNTLKEESKKKALDEPVDDMPWQFWSHL MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .....................I...........................R.................................................. 510 520 530 540 550 560 570 580 590 600 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) LLMIDGWLLEKKLTLDDAKLLRDMVWKRERRIHDIYLECKEKNEHEAVSMFLKLTSSPKSQGLYVVHIAAEMAPVAKVGGLGDVVTGLGKALQKRGHLVE MeSSIV (Do DF) .......................L............................................................................ MeSSiv (KM140) .......................................R............................................................ 610 620 630 640 650 660 670 680 690 700 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) IILPKYDCMQYDGIGNLRALDVVLESYFDGKLYKNEVWVGTIEGLPVYFIEPHHPGKFFWRGQFYGEHDDFKRFSFFSRAALELLLQAGKKPDIIHCHDW MeSSIV (Do DF) ...........................................................S........................................ MeSSiv (KM140) .................................................................................................... 710 720 730 740 750 760 770 780 790 800 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) QTAFVAPLYWDIYAPKGLNSARICFTCHNFEYQGSAPASELASCGLDVQQLNRPDRMQDNSAHDRINPIKGAVVFSNIVTTVSPTYAQEVRTSEGGKGLH MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 810 820 830 840 850 860 870 880 890 900 168 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) STLNFHAKKFIGILNGIDTDVWNPATDTLLEVQYNANDLQGKAENKIATRQHLGLSTADARQPLVGCITRLVPQKGVHLIRHAIYRTLELGGQFLLLGSS MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) .................................................................................................... 910 920 930 940 950 960 970 980 990 1000 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) PVAHIQREFEGIANHFQNHEHIRLVLKYDESLAHSIYAASDMFIIPSIFEPCGLTQMIAMRYGSIPIARKTGGLNDSVFDVDDDTIPLQFRNGYTFLNPD MeSSIV (Do DF) .................................................................................................... MeSSiv (KM140) ..............................................................................L..................... 1010 1020 1030 1040 1050 1060 1070 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| MeSSiv (Manes.15G118600.1) EQGVNSALERAFNHYRNDPESWQQLVQRDMDIDFSWESSASQYEELYSKSVARARAAASRS*LW*MAFSWKAKDX MeSSIV (Do DF) .............................................................*..*.......... MeSSiv (KM140) ...........................K..N..............................*..*.......... 169 4. Kết quả xác định trình tự promoter C54 đã phân lập (so sánh với trình tự truy vấn C54 (AY217353)) và kết quả blasn (NCBI) 10 20 30 40 50 60 70 80 90 100 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen ggatccacgggtgtgggccaactccaccgcccaagagaaaacctaaaatggagaaatttgtcaaagctttttcaactttacagactaggcataggtcctg C54 Promoter -----------......................................................................................... 110 120 130 140 150 160 170 180 190 200 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen gggcaaacagaacagagctcaggacgctgcctccctgcttgcccacctggaatcattgttctccgcttcctgcttatgtcatattctccgatgatttttt C54 Promoter .................................................................................................... 210 220 230 240 250 260 270 280 290 300 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen attttttaattttgcgtttctctttctttgcaggtaaaataagcccatctcaaattcagatttgggtaatcatgcgatttgagaaacattatagaaaaaa C54 Promoter .................................................................................................... 310 320 330 340 350 360 370 380 390 400 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen ggtgatcaactccaattgtgtgataattttgtagatgtggcaattaaggtcggtttggcttttataagaaattagaattaaccaatttcataattatatc C54 Promoter ...........................................................................R........................ 410 420 430 440 450 460 470 480 490 500 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen attcatgactattgattataatatctgacaaatcctgtaatgatttttaacccatttcagtatgatttactcatcccattctttatggctgcttcaatat C54 Promoter ............Y....................................................................................... 510 520 530 540 550 560 570 580 590 600 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen acagagttcatgacatgtgaatctgaatttaaatattaatttctctgtttaattaataattaataatccatttcatataatttttttaaattaattaatt C54 Promoter .................................................................................................... 610 620 630 640 650 660 670 680 690 700 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen aattaaagaagaaaataagaagctgggaacatgataataataaaggtcacctacattaccgactgcacaaagcacataagcacaaaaaaccaaaagaaag C54 Promoter .................................................................................................... 710 720 730 740 750 760 770 780 790 800 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen atgatggcttagctgaaaaaggccctccattatttacacttttcgatcaaaaacaaatatcatgggtaatttaaaaagataaagtacagcttacttattt C54 Promoter ...................t.........................................TA....................C......Y......... 810 820 830 840 850 860 870 880 890 900 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen gaaaaaaaatttttaattcacnattcttaaaatgttgatntattcctattgattttgatttcagagagatgtgaggtcaaatcccaagattcctcttcct C54 Promoter .................................................................................................... 910 920 930 940 950 960 970 980 990 1000 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....| C54 Referen taggtagagttcccacctctctccctctcacatgaagcttactggcctctactcctctctctatataagccactctcagttaccctctctctgcaccaaa C54 Promoter .................................................................................................... 1010 1020 1030 1040 1050 1060 1070 1080 ....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|....|.... C54 Referen tacactctaagctctctcttcacttcctctttgcttctcaccatttacttttagtttctatttcattttctcttgctttctatg C54 Promoter .................................................... 170 5. Kết quả phân tích promoter C54 bằng phần mềm TSSP C54 Promoter (sắn đỏ địa phương) C54 Promoter (Tham chiếu) > test sequence Length of sequence- 1041 Thresholds for TATA+ promoters - 0.02, for TATA-/enhancers - 0.04 2 promoter/enhancer(s) are predicted Promoter Pos: 981 LDF- 0.14 TATA box at 947 21.81 Enhancer Pos: 993 LDF- 0.06 Transcription factor binding sites/RegSite DB: for promoter at position - 981 815 (+) RSP00005 CTWWWWWWGT 979 (+) RSP00016 caTGCAC 876 (-) RSP00026 gcttttgaTGACtTcaaacac 943 (+) RSP00046 atccattcTATATAAGaaacata 790 (+) RSP00098 cttaatatATTTTTAattattttattctcttaa 794 (+) RSP00117 aattATTTTTAaa 795 (+) RSP00125 aatATTTTTAtt 786 (+) RSP00126 gaaaattttaatATTTTTAtttagtatt 795 (+) RSP00135 aatATTTTTAtc 794 (+) RSP00140 aattATTTTTAtt 681 (+) RSP00161 WAAAG 763 (+) RSP00161 WAAAG 732 (-) RSP00161 WAAAG 966 (+) RSP00240 CAGTTA 918 (+) RSP00252 CACATG 826 (-) RSP00308 CAACA 798 (+) RSP00339 RTTTTTR 743 (-) RSP00339 RTTTTTR 864 (+) RSP00383 GGTCAAA 787 (+) RSP00398 TTTGAA 863 (+) RSP00467 tGGTCAatTc 862 (+) RSP00468 ttGGTCAatTc 908 (-) RSP00469 GNGGTG 759 (+) RSP00477 TTTAA 801 (+) RSP00477 TTTAA 820 (-) RSP00477 TTTAA 764 (-) RSP00477 TTTAA 796 (+) RSP00508 gcaTTTTTatca 801 (-) RSP00508 gcaTTTTTatca 800 (-) RSP00508 gcaTTTTTatca 799 (-) RSP00508 gcaTTTTTatca 798 (-) RSP00508 gcaTTTTTatca 769 (-) RSP00508 gcaTTTTTatca 745 (-) RSP00508 gcaTTTTTatca 681 (-) RSP00508 gcaTTTTTatca 703 (-) RSP00512 cttgtaacCATCAgccaatcgaccagccaatcattc > test sequence Length of sequence- 1073 Thresholds for TATA+ promoters - 0.02, for TATA-/enhancers - 0.04 2 promoter/enhancer(s) are predicted Promoter Pos: 981 LDF- 0.14 TATA box at 947 21.81 Enhancer Pos: 1004 LDF- 0.08 Transcription factor binding sites/RegSite DB: for promoter at position - 981 815 (+) RSP00005 CTWWWWWWGT 979 (+) RSP00016 caTGCAC 876 (-) RSP00026 gcttttgaTGACtTcaaacac 943 (+) RSP00046 atccattcTATATAAGaaacata 794 (+) RSP00117 aattATTTTTAaa 795 (+) RSP00125 aatATTTTTAtt 795 (+) RSP00135 aatATTTTTAtc 794 (+) RSP00140 aattATTTTTAtt 681 (+) RSP00161 WAAAG 706 (+) RSP00161 WAAAG 763 (+) RSP00161 WAAAG 769 (+) RSP00161 WAAAG 732 (-) RSP00161 WAAAG 966 (+) RSP00240 CAGTTA 918 (+) RSP00252 CACATG 774 (-) RSP00265 ACTTTA 711 (-) RSP00305 CCTTTT 826 (-) RSP00308 CAACA 798 (+) RSP00339 RTTTTTR 743 (-) RSP00339 RTTTTTR 864 (+) RSP00383 GGTCAAA 787 (+) RSP00398 TTTGAA 863 (+) RSP00467 tGGTCAatTc 862 (+) RSP00468 ttGGTCAatTc 908 (-) RSP00469 GNGGTG 759 (+) RSP00477 TTTAA 801 (+) RSP00477 TTTAA 820 (-) RSP00477 TTTAA 764 (-) RSP00477 TTTAA 796 (+) RSP00508 gcaTTTTTatca 801 (-) RSP00508 gcaTTTTTatca 800 (-) RSP00508 gcaTTTTTatca 799 (-) RSP00508 gcaTTTTTatca 798 (-) RSP00508 gcaTTTTTatca 769 (-) RSP00508 gcaTTTTTatca 745 (-) RSP00508 gcaTTTTTatca 712 (-) RSP00508 gcaTTTTTatca 681 (-) RSP00508 gcaTTTTTatca 171 for promoter at position - 993 815 (+) RSP00005 CTWWWWWWGT 979 (+) RSP00016 caTGCAC 876 (-) RSP00026 gcttttgaTGACtTcaaacac 943 (+) RSP00046 atccattcTATATAAGaaacata 790 (+) RSP00098 cttaatatATTTTTAattattttattctcttaa 794 (+) RSP00117 aattATTTTTAaa 795 (+) RSP00125 aatATTTTTAtt 786 (+) RSP00126 gaaaattttaatATTTTTAtttagtatt 795 (+) RSP00135 aatATTTTTAtc 794 (+) RSP00140 aattATTTTTAtt 763 (+) RSP00161 WAAAG 732 (-) RSP00161 WAAAG 966 (+) RSP00240 CAGTTA 918 (+) RSP00252 CACATG 826 (-) RSP00308 CAACA 798 (+) RSP00339 RTTTTTR 743 (-) RSP00339 RTTTTTR 864 (+) RSP00383 GGTCAAA 787 (+) RSP00398 TTTGAA 863 (+) RSP00467 tGGTCAatTc 862 (+) RSP00468 ttGGTCAatTc 908 (-) RSP00469 GNGGTG 759 (+) RSP00477 TTTAA 801 (+) RSP00477 TTTAA 820 (-) RSP00477 TTTAA 764 (-) RSP00477 TTTAA 796 (+) RSP00508 gcaTTTTTatca 801 (-) RSP00508 gcaTTTTTatca 800 (-) RSP00508 gcaTTTTTatca 799 (-) RSP00508 gcaTTTTTatca 798 (-) RSP00508 gcaTTTTTatca 769 (-) RSP00508 gcaTTTTTatca 745 (-) RSP00508 gcaTTTTTatca 703 (-) RSP00512 cttgtaacCATCAgccaatcgaccagccaatcattc 703 (-) RSP00512 cttgtaacCATCAgccaatcgaccagccaatcattc for promoter at position - 1004 815 (+) RSP00005 CTWWWWWWGT 979 (+) RSP00016 caTGCAC 876 (-) RSP00026 gcttttgaTGACtTcaaacac 943 (+) RSP00046 atccattcTATATAAGaaacata 794 (+) RSP00117 aattATTTTTAaa 795 (+) RSP00125 aatATTTTTAtt 795 (+) RSP00135 aatATTTTTAtc 794 (+) RSP00140 aattATTTTTAtt 706 (+) RSP00161 WAAAG 763 (+) RSP00161 WAAAG 769 (+) RSP00161 WAAAG 732 (-) RSP00161 WAAAG 966 (+) RSP00240 CAGTTA 918 (+) RSP00252 CACATG 774 (-) RSP00265 ACTTTA 711 (-) RSP00305 CCTTTT 826 (-) RSP00308 CAACA 798 (+) RSP00339 RTTTTTR 743 (-) RSP00339 RTTTTTR 864 (+) RSP00383 GGTCAAA 787 (+) RSP00398 TTTGAA 863 (+) RSP00467 tGGTCAatTc 862 (+) RSP00468 ttGGTCAatTc 908 (-) RSP00469 GNGGTG 759 (+) RSP00477 TTTAA 801 (+) RSP00477 TTTAA 820 (-) RSP00477 TTTAA 764 (-) RSP00477 TTTAA 796 (+) RSP00508 gcaTTTTTatca 801 (-) RSP00508 gcaTTTTTatca 800 (-) RSP00508 gcaTTTTTatca 799 (-) RSP00508 gcaTTTTTatca 798 (-) RSP00508 gcaTTTTTatca 769 (-) RSP00508 gcaTTTTTatca 745 (-) RSP00508 gcaTTTTTatca 712 (-) RSP00508 gcaTTTTTatca 172 Phụ lục 3 Sơ đồ các vector mang gen SSIV được tổng hợp nhân tạo pK7WG2D-35S:SSIV:T35 pBI121-C54:SSIV:NOST 173 Phụ lục 4 Môi trường MS (Murashige and Skoog, 1962) STT Thành phần Hàm lượng (mg/l) 1 CaCl2 CaCl2.6H2O 332,02 440 2 KH2PO4 170 3 KNO3 1900,00 4 MgSO4 180,54 5 NH4NO3 1650,00 6 FeSO4.7H2O EDTA Hoặc FeNaEDTA 27,8 37,3 36,7 7 H3BO3 6,2 8 KI 0,83 9 MnSO4.2H2O 16,9 10 ZnSO4 8,6 11 CoCl2.6H2O 0,025 12 CuSO4.5H2O 0,025 13 NaMoO4.2H2O 0,25 14 MnSO4.H2O 16,9 15 Glycin 2,00 16 Thiamin HCl 0,10 17 Myo – innositol 100,00 18 Pyridoxine HCl 0,50