Luận án Nghiên cứu đặc điểm thực vật, thành phần hóa học và một số tác dụng sinh học của loài muồng lùn [Chamaecrista pumila (Lam.) K.Larsen]

Ở nồng độ 10 μg/ml thì cao phân đoạn ethylacetat được xem là ức chế SOD mạnh nhất (58,91%), so với còn 5 mẫu cao còn lại nhưng tác dụng ức chế SOD không tốt bằng tác dụng của quercetin (với tỷ lệ ức chế đạt 95,50%). 6 mẫu cao này tiếp tục được xác định IC50 với quercetin là chất đối chiếu thu được kết quả như sau: IC50: Cao ethanol = 12,33μg/ml; cao phân đoạn n- hexan = 94,03μg/ml; cao phân đoạn dicloromethan = 25,26μg/ml; cao ethyl cacetat = 8,382/ml ; cao phân đoạn n- butanol; Cao nước không tính do liều IC50 quá lớn. Kết quả cho thấy khả năng dọn gốc tự do SOD của cao phân đoạn ethyl acetat là mạnh nhất Từ hai thí nghiệm thử tác dụng dọn gốc tự do DPDH và SOD thấy được rằng cao chiết muồng lùn có tác dụng chống oxy hóa và khả năng chống oxy hóa mạnh nhất là ở cao phân đoạn ethyl acetat. Các hợp chất phân lập được chưa được đánh giá tác dụng chống oxy hóa in vitro tuy nhiên, tổng quan tài liệu cho kết quả, một số hợp chất trong số này thể hiện tác dụng chống oxy hóa thông qua một số cơ chế khác nhau. Hợp chất TB6.4 (Liquiritigenin) có tác dụng chống oxy hóa in vitro thông qua khả năng ức chế hình thành gốc tự do ABTS•+ [20]. Về cơ chế tác dụng, trong tế bào chất, protein KEAP1 thường được liên kết với NRF2 để ổn định nó trong tế bào chất. Liquiritigenin liên kết với KEAP1, khiến NRF2 giải phóng khỏi phức hợp protein và chuyển vị trí vào nhân. Trong nhân, NRF2 liên kết với các yếu tố phản ứng chống oxy hóa (AREs), bắt đầu phản ứng chống oxy hóa, do đó làm giảm sản xuất các gốc oxy phản ứng quá mức ( ROS) nội bào và làm giảm độc tính tế bào do quá trình oxy hóa gây ra [141]. Hợp chất TB3.10 thể hiện tác dụng chống oxy hóa thông qua tác dụng khử tyrosinase [86], loại bỏ gốc tự do DPPH và superoxide, ức chế xanthine oxidase, ức chế sản xuất MMP-1 [80]; điều chỉnh giảm sự biểu hiện của gen tiền apoptotic Txnip và Ddit3 và ngoài ra còn điều chỉnh tăng biểu hiện của các gen phản ứng chống oxy hóa Hmox1, Nqo1 và Sod1 [96]. Trong một nghiên cứu của Suresh G và các cộng sự (2012), hợp chất TB12.10 ((-)- festidinol) được phân lập trong cao chiết methanol của loài Dichrostachys cinerea đã được đánh giá khả năng tách gốc tự do và ức chế sản phẩm glycation tiên tiến (AGE); (-)- festidinol được tìm thấy là chất ức chế AGE tự nhiên mới [117]. Dựa trên cơ sở kết quả đánh giá tác dụng chống oxy hóa trên in vitro chúng tôi tiếp tục tiến hành thử tác dụng dược lý trên động vật thực nghiệm và phân lập chất hóa học.

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