Luận án Nghiên cứu đánh giá biến động các thông số ảnh hưởng đến chất lượng tôm sú (penaeus monodon) bảo quản ở 0 độ C sau thu hoạch

Tôm đặt trên mâm trắng. Mâm tôm đặt trên mặt bàn đá bằng phẳng. Tôm đặt sao cho đầu tôm nằm ngang, song song với cạnh bàn. Kéo thân tôm vuông góc với đầu tôm, đuôi hơi cong theo chiều cong tự nhiên của tôm. Máy ảnh đặt trên giá đỡ cố định cách mẫu 30 cm, đặt vuông góc với mẫu. Chế độ chụp: không zoom, bật flash, độ phân giải 8.3 megapixel. Máy ảnh: Sử dụng máy ảnh Panasonic Lumix DMC- FS4

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PHỤ LỤC Phụ lục A: Bảng A.2 Một số quy định về giới hạn hàm lượng histamine trong thủy sản Khu vực Giới hạn [27] Châu Âu 200 mg/kg Cục Quản lý Thực phẩm và Dược phẩm – Hoa Kỳ 50 mg/kg Australia, Germany and New Zealand Food Standards Code (FSC) 200 mg/kg Nam Phi – Ý 100 mg/kg Bảng A.3 Một số nghiên cứu sử dụng amine sinh học để đánh giá độ tươi Mẫu Tên khoa học Chỉ số amine sinh học Tài liệu Cá ngừ - HI+PU+CA/SM+SD [159] Cá ngừ Thunnus thynnus HI+CA+PU+TY [258] Cá chép Cyprinus carpio PU và CA [134] Cá hồi Salmo gairdneri CA [266] Cá mồi Sardina pilchardus HI+PU+CA/SM+SD và HI+CA+PU+TY [186] Cá chẻm Lates calcarifer HI+PU+CA/SM+SD và HI+CA+PU+TY [25] Cá thu Merluccius merluccius, L CA và AG [216] Cá hồi Oncorhynchus keta PU và CA [210] Với HI: histamine, PU: putrescine, CA: cadaverine, SM: spermine, SD: permidine, TY: tyramine, AG: agmatine. Bảng A.4 Bảng phân loại chất lượng cá ngừ theo chỉ số histamine [160] Mẫu Khoảng/ Trung bình Phân loại Histamine (mg/kg) Mẫu cá xác thực chất lượng Khoảng Tốt 1,28 – 9,8 Trung bình 3,84 Khoảng Chấp nhận 6,44 – 58,34 Trung bình 23,58 Khoảng Không chấp nhận 87,42 – 507,3 Trung bình 253,2 Mẫu cá bán trên thị trường Khoảng Tốt 0,28 – 4,02 Trung bình 1,41 Khoảng Chấp nhận 8,12 – 80,28 Trung bình 37,09 Bảng A.5 Tương quan giữa chất lượng cảm quan và hàm lượng hypoxanthine theo ngày bảo quản trong nghiên cứu của Mol [161] Ngày bảo quản Điểm cảm quan Hx (µM/gam) 0 9,9 2,63 7 9,2 2,86 14 8,1 3,38 21 4,7 4,50 28 1,8 4,96 PHỤ LỤC B HÌNH ẢNH TÔM SÚ (Penaeus monodon) ĐÁNH GIÁ Mẫu: Tôm sú (Penaeus monodon) sau khi lấy khỏi thùng giữ nhiệt được rửa qua bằng nước sạch rồi đặt lên mâm để chụp ngay Điều kiện chụp: Tôm đặt trên mâm trắng. Mâm tôm đặt trên mặt bàn đá bằng phẳng. Tôm đặt sao cho đầu tôm nằm ngang, song song với cạnh bàn. Kéo thân tôm vuông góc với đầu tôm, đuôi hơi cong theo chiều cong tự nhiên của tôm. Máy ảnh đặt trên giá đỡ cố định cách mẫu 30 cm, đặt vuông góc với mẫu. Chế độ chụp: không zoom, bật flash, độ phân giải 8.3 megapixel. Máy ảnh: Sử dụng máy ảnh Panasonic Lumix DMC- FS4 Thông tin chung Hãng sản xuất Panasonic FH / Fs Series Độ lớn màn hình LCD (inch) 2.5 inch Màu sắc Đen Trọng lượng Camera 112g Kích cỡ máy (Dimensions) 55.4 x 97.6 x 22.3 mm Loại thẻ nhớ Multimedia Card (MMC) Secure Digital Card (SD) SD High Capacity (SDHC) Bộ nhớ trong (Mb) 50 Cảm biến hình ảnh Bộ cảm biến hình ảnh (Image Sensor) 1/2.5 Megapixel (Số điểm ảnh hiệu dụng) 8.3Megapixel Độ nhạy sáng (ISO) Auto, 80, 100, 200, 400, 800, 1000, (In High Sensitivity mode: 1600-6400) Độ phân giải ảnh lớn nhất 3264 x 2448 Thông số về Lens Độ dài tiêu cự (Focal Length) f = 5.5 - 22.0mm (35mm equiv.: 33 - 132mm) Độ mở ống kính (Aperture) Wide: F2.8 - F8.0, Tele: F5.9 - 16.0 Tốc độ chụp (Shutter Speed) 8-1/2000 sec. (in Starry Sky mode: 15/30/60 sec.) Tự động lấy nét (AF) Optical Zoom (Zoom quang) 4x Digital Zoom (Zoom số) 4.0x Thông số khác Định dạng File ảnh JPEG Định dạng File phim QUICKTIME video format Chuẩn giao tiếp USB AV out PictBridge Điều kiện phòng thí nghiệm Ánh sáng trắng từ đèn huỳnh quang phân bố đều. 6 bóng 1m2 cho diện tích phòng 2×5m, chiều cao 2 m2. Phòng kính cách âm, nhiệt độ phòng 20℃. PHỤ LỤC C HÌNH ẢNH THÍ NGHIỆM Hình C1. Tôm thu hoạch tại vựa Hình C2. Tôm sau khi bảo quản trong túi zipper Hình C3. Chuẩn bị mẫu tôm Hình C4. Người thử đang tiến hành thí nghiệm Hình C5. Người thử Hình C6. Huấn luyện hội đồng Hình C7. Chuẩn bị mẫu tôm cho đợt thí nghiệm đánh giá QIM PHỤ LỤC D: Sắc ký đồ chuẩn hypoxanthine Sắc ký đồ chuẩn 0,01ppm Sắc ký đồ chuẩn 0,05 ppm Sắc ký đồ chuẩn 0,1ppm Sắc ký đồ chuẩn 0,5 ppm Sắc ký đồ chuẩn 1ppm Sắc ký đồ chuẩn 5 ppm PHỤ LỤC E Sắc ký đồ chuẩn histamine Sắc ký đồ chuẩn 0,055 ppm Sắc ký đồ chuẩn 0,110 ppm Sắc ký đồ chuẩn 0,165 ppm Sắc ký đồ chuẩn 0,220 ppm Sắc ký đồ chuẩn 0,330 ppm

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