Tôm đặt trên mâm trắng. Mâm tôm đặt trên mặt bàn đá bằng phẳng. Tôm đặt sao cho đầu tôm nằm ngang, song song với cạnh bàn. Kéo thân tôm vuông góc với đầu tôm, đuôi hơi cong theo chiều cong tự nhiên của tôm.
Máy ảnh đặt trên giá đỡ cố định cách mẫu 30 cm, đặt vuông góc với mẫu.
Chế độ chụp: không zoom, bật flash, độ phân giải 8.3 megapixel.
Máy ảnh: Sử dụng máy ảnh Panasonic Lumix DMC- FS4
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Tài liệu khác
267
(2015).
PHỤ LỤC
Phụ lục A:
Bảng A.2 Một số quy định về giới hạn hàm lượng histamine trong thủy sản
Khu vực
Giới hạn [27]
Châu Âu
200 mg/kg
Cục Quản lý Thực phẩm và Dược phẩm – Hoa Kỳ
50 mg/kg
Australia, Germany and New Zealand Food Standards Code (FSC)
200 mg/kg
Nam Phi – Ý
100 mg/kg
Bảng A.3 Một số nghiên cứu sử dụng amine sinh học để đánh giá độ tươi
Mẫu
Tên khoa học
Chỉ số amine sinh học
Tài liệu
Cá ngừ
-
HI+PU+CA/SM+SD
[159]
Cá ngừ
Thunnus thynnus
HI+CA+PU+TY
[258]
Cá chép
Cyprinus carpio
PU và CA
[134]
Cá hồi
Salmo gairdneri
CA
[266]
Cá mồi
Sardina pilchardus
HI+PU+CA/SM+SD và
HI+CA+PU+TY
[186]
Cá chẻm
Lates calcarifer
HI+PU+CA/SM+SD và
HI+CA+PU+TY
[25]
Cá thu
Merluccius merluccius, L
CA và AG
[216]
Cá hồi
Oncorhynchus keta
PU và CA
[210]
Với HI: histamine, PU: putrescine, CA: cadaverine, SM: spermine, SD: permidine, TY: tyramine, AG: agmatine.
Bảng A.4 Bảng phân loại chất lượng cá ngừ theo chỉ số histamine [160]
Mẫu
Khoảng/ Trung bình
Phân loại
Histamine (mg/kg)
Mẫu cá xác thực chất lượng
Khoảng
Tốt
1,28 – 9,8
Trung bình
3,84
Khoảng
Chấp nhận
6,44 – 58,34
Trung bình
23,58
Khoảng
Không chấp nhận
87,42 – 507,3
Trung bình
253,2
Mẫu cá bán trên thị trường
Khoảng
Tốt
0,28 – 4,02
Trung bình
1,41
Khoảng
Chấp nhận
8,12 – 80,28
Trung bình
37,09
Bảng A.5 Tương quan giữa chất lượng cảm quan và hàm lượng hypoxanthine theo ngày bảo quản trong nghiên cứu của Mol [161]
Ngày bảo quản
Điểm cảm quan
Hx (µM/gam)
0
9,9
2,63
7
9,2
2,86
14
8,1
3,38
21
4,7
4,50
28
1,8
4,96
PHỤ LỤC B
HÌNH ẢNH TÔM SÚ (Penaeus monodon) ĐÁNH GIÁ
Mẫu:
Tôm sú (Penaeus monodon) sau khi lấy khỏi thùng giữ nhiệt được rửa qua bằng nước sạch rồi đặt lên mâm để chụp ngay
Điều kiện chụp:
Tôm đặt trên mâm trắng. Mâm tôm đặt trên mặt bàn đá bằng phẳng. Tôm đặt sao cho đầu tôm nằm ngang, song song với cạnh bàn. Kéo thân tôm vuông góc với đầu tôm, đuôi hơi cong theo chiều cong tự nhiên của tôm.
Máy ảnh đặt trên giá đỡ cố định cách mẫu 30 cm, đặt vuông góc với mẫu.
Chế độ chụp: không zoom, bật flash, độ phân giải 8.3 megapixel.
Máy ảnh: Sử dụng máy ảnh Panasonic Lumix DMC- FS4
Thông tin chung
Hãng sản xuất
Panasonic FH / Fs Series
Độ lớn màn hình LCD (inch)
2.5 inch
Màu sắc
Đen
Trọng lượng Camera
112g
Kích cỡ máy (Dimensions)
55.4 x 97.6 x 22.3 mm
Loại thẻ nhớ
Multimedia Card (MMC)
Secure Digital Card (SD)
SD High Capacity (SDHC)
Bộ nhớ trong (Mb)
50
Cảm biến hình ảnh
Bộ cảm biến hình ảnh (Image Sensor)
1/2.5
Megapixel (Số điểm ảnh hiệu dụng)
8.3Megapixel
Độ nhạy sáng (ISO)
Auto, 80, 100, 200, 400, 800, 1000, (In High Sensitivity mode: 1600-6400)
Độ phân giải ảnh lớn nhất
3264 x 2448
Thông số về Lens
Độ dài tiêu cự (Focal Length)
f = 5.5 - 22.0mm (35mm equiv.: 33 - 132mm)
Độ mở ống kính (Aperture)
Wide: F2.8 - F8.0, Tele: F5.9 - 16.0
Tốc độ chụp (Shutter Speed)
8-1/2000 sec. (in Starry Sky mode: 15/30/60 sec.)
Tự động lấy nét (AF)
Optical Zoom (Zoom quang)
4x
Digital Zoom (Zoom số)
4.0x
Thông số khác
Định dạng File ảnh
JPEG
Định dạng File phim
QUICKTIME video format
Chuẩn giao tiếp
USB
AV out
PictBridge
Điều kiện phòng thí nghiệm
Ánh sáng trắng từ đèn huỳnh quang phân bố đều. 6 bóng 1m2 cho diện tích phòng 2×5m, chiều cao 2 m2. Phòng kính cách âm, nhiệt độ phòng 20℃.
PHỤ LỤC C
HÌNH ẢNH THÍ NGHIỆM
Hình C1. Tôm thu hoạch tại vựa
Hình C2. Tôm sau khi bảo quản trong túi zipper
Hình C3. Chuẩn bị mẫu tôm
Hình C4. Người thử đang tiến hành thí nghiệm
Hình C5. Người thử
Hình C6. Huấn luyện hội đồng
Hình C7. Chuẩn bị mẫu tôm cho đợt thí nghiệm đánh giá QIM
PHỤ LỤC D:
Sắc ký đồ chuẩn hypoxanthine
Sắc ký đồ chuẩn 0,01ppm
Sắc ký đồ chuẩn 0,05 ppm
Sắc ký đồ chuẩn 0,1ppm
Sắc ký đồ chuẩn 0,5 ppm
Sắc ký đồ chuẩn 1ppm
Sắc ký đồ chuẩn 5 ppm
PHỤ LỤC E
Sắc ký đồ chuẩn histamine
Sắc ký đồ chuẩn 0,055 ppm
Sắc ký đồ chuẩn 0,110 ppm
Sắc ký đồ chuẩn 0,165 ppm
Sắc ký đồ chuẩn 0,220 ppm
Sắc ký đồ chuẩn 0,330 ppm