Nghiên cứu đánh giá sự đa dạng di truyền các giống/dòng chè (Camellia sinensis(L.) O. Kuntze) ởViệt Nam bằng chỉthịhình thái và chỉthịphân tửmicrosatellite (SSR)

54 1.50 1.22 1.81 1.39 1.82 1.22 1.24 1.37 1.06 MinhRongNo1 1.46 1.07 1.37 1.32 1.32 1.68 1.92 1.32 1.48 1.29 1.17 1.36 1.02 1.72 0.98 1.24 1.72 1.32 1.66 1.22 1.68 1.12 1.57 1.16 MinhRongNo3 1.45 1.37 2.74 1.45 1.30 1.88 1.90 1.44 2.16 1.40 1.40 1.47 1.36 1.70 0.88 1.12 1.41 1.31 1.13 1.20 1.66 1.46 1.84 1.84 TB14 1.30 1.28 1.39 1.49 1.20 1.36 2.63 1.11 1.79 0.90 0.82 1.15 1.15 1.73 1.40 1.37 1.73 1.11 1.67 1.24 1.22 1.24 1.37 1.31 ShanTanChi 1.63 1.02 1.61 1.42 0.91 1.62 1.87 1.15 1.03 1.01 1.37 1.44 1.48 2.07 1.22 1.19 2.07 1.15 1.10 1.06 1.62 0.88 0.96 1.24 ToHieu 1.80 0.99 1.89 1.48 1.48 2.02 2.44 1.15 2.00 1.81 1.40 2.16 0.96 1.26 1.68 1.18 2.64 1.28 1.49 1.17 1.73 1.30 2.10 1.97 HoocMon 1.27 1.07 1.46 1.46 1.68 1.99 1.03 1.60 1.06 1.56 1.56 1.29 1.19 2.62 1.19 1.45 1.93 2.11 1.18 1.03 1.48 1.14 1.38 0.97 1A 1.64 1.19 1.33 1.14 1.32 1.57 1.59 0.99 0.93 1.94 1.43 1.72 0.60 1.39 0.94 0.91 1.79 1.97 2.43 1.30 1.01 1.48 1.53 1.12 5A 1.13 0.83 1.91 1.32 0.81 1.53 1.37 0.95 1.33 1.14 0.91 0.98 1.23 2.67 1.24 0.98 2.67 1.31 1.51 0.87 1.34 1.32 1.42 1.14 F16 1.37 0.98 1.98 1.10 0.98 1.41 2.53 1.23 1.17 1.20 1.33 1.55 1.04 1.63 1.43 1.15 2.04 1.52 1.06 0.93 1.41 1.03 1.49 1.07 F35 1.70 0.92 1.67 0.88 1.08 0.90 2.63 1.34 1.28 1.30 1.08 1.50 0.70 1.73 1.08 0.81 2.14 1.63 1.16 1.13 1.36 1.13 1.18 1.31 LCT 1.76 1.75 2.14 1.55 1.26 1.28 1.99 2.78 2.25 1.65 1.14 1.72 1.10 1.50 1.75 1.72 2.20 2.78 1.45 1.70 1.97 2.11 1.24 1.93 LDPNo2 0.00 1.46 1.04 0.00 1.55 1.57 1.99 1.17 0.86 1.36 1.65 1.21 1.10 0.00 1.75 2.82 0.00 1.68 1.45 2.11 1.28 1.01 1.24 2.62 PHNo1 1.75 1.05 2.54 1.25 1.25 1.68 1.30 1.24 1.26 1.20 1.07 1.42 1.03 2.60 1.17 1.27 2.60 1.57 0.93 1.25 1.46 1.12 0.79 1.92 PHT 1.47 1.17 1.15 0.85 1.10 1.68 2.39 1.10 1.04 0.84 1.07 1.14 1.16 1.90 0.61 1.61 1.50 1.57 1.44 1.00 1.68 1.12 1.13 0.72 S28 1.39 1.32 1.99 0.89 1.58 1.27 1.85 1.13 1.70 1.69 1.22 1.57 0.69 1.65 0.76 0.80 1.65 1.54 1.30 1.05 1.61 1.40 1.50 1.38 TrungDuThanhDuc 1.88 0.81 1.98 1.23 0.98 1.12 2.53 0.83 1.39 0.88 0.57 0.86 1.16 1.63 0.74 1.15 0.00 0.75 1.57 1.25 1.00 1.25 1.26 0.95 TrungDuTim 1.21 0.91 0.90 1.00 1.18 1.71 1.45 1.32 0.78 1.28 1.10 1.35 1.24 2.34 0.91 1.57 2.34 1.54 2.28 1.15 1.20 1.00 1.10 1.67 TrungDuVang 1.38 1.22 0.85 1.35 0.88 1.38 1.62 1.02 0.78 1.12 1.27 1.19 1.64 2.52 1.09 1.34 1.42 1.49 1.76 1.17 0.91 0.82 1.27 0.99 TrungDuXanh 2.11 1.58 2.49 1.38 1.38 1.07 0.00 1.34 2.60 1.99 1.71 1.78 1.26 1.85 1.40 1.55 2.54 1.74 2.49 1.20 1.40 1.07 1.30 1.18 TrungDuXanhHonHop 1.27 0.87 1.87 1.12 0.99 1.01 1.73 0.90 1.29 1.09 0.87 1.45 1.05 2.62 1.33 0.94 1.93 1.01 1.46 0.92 1.14 1.14 1.38 1.10 BanXenNo2 2.45 1.75 1.04 1.26 1.55 1.97 1.30 1.68 1.15 1.36 1.36 1.02 2.02 1.10 1.24 2.12 1.50 1.68 1.04 1.70 1.28 1.41 1.65 1.52 MocChau 1.48 1.40 2.08 1.49 1.89 1.22 1.94 1.22 1.79 2.28 1.99 1.84 0.94 1.73 1.28 0.97 1.73 1.22 1.39 0.73 1.36 0.95 2.28 1.06 SuoiGiang 1.90 1.60 1.59 1.58 1.40 1.02 1.45 1.03 1.01 1.22 1.10 1.06 0.96 1.65 0.76 1.29 1.36 1.13 1.59 1.27 1.27 0.95 1.28 0.97 ShanTuyetMocChau 1.63 1.22 1.32 0.91 1.13 1.15 1.46 0.87 0.87 1.24 1.37 1.44 0.71 1.67 1.11 1.08 1.67 1.15 1.32 0.88 0.84 1.06 1.81 0.79 Ph uc D in hN o2 Ph uc D in hN o3 Ph uc Va nN o1 0 Ph uc Va nT ie n PT 95 Q ua ng Ta y Th ie tB ao Tr a Tr ie tG ia ng N o1 Tr ie tG ia ng N o2 Tr ie tG ia ng N o3 Tr ie tG ia ng N o4 Tr ie tG ia ng N o5 Tr un gQ uo cL aN ho H uo ng Ti ch So nN o1 Q ue Lu c Va nN am Th am Ve Ch at Ti en Cu D eP hu ng G ia Va i Lu ng Ph in N am N ga t N am Ty B' La o PhucDinhNo2 0.00 PhucDinhNo3 0.67 0.00 PhucVanNo10 0.67 0.42 0.00 PhucVanTien 0.93 0.86 0.44 0.00 PT95 0.70 0.99 0.48 0.32 0.00 QuangTay 0.91 1.16 0.83 1.01 0.71 0.00 ThietBaoTra 0.95 1.33 1.05 0.95 0.82 0.93 0.00 TrietGiangNo1 0.96 1.23 1.06 1.18 0.89 0.60 0.97 0.00 TrietGiangNo2 0.92 1.06 0.65 0.91 0.94 1.11 1.28 0.65 0.00 TrietGiangNo3 1.06 1.21 1.04 1.15 1.19 1.27 0.95 0.67 0.22 0.00 TrietGiangNo4 0.78 0.90 0.71 0.79 0.48 0.69 0.81 1.00 0.96 1.10 0.00 TrietGiangNo5 0.80 0.67 0.49 0.61 0.65 0.53 1.00 1.11 1.07 1.34 0.49 0.00 TrungQuocLaNho 1.46 2.18 1.85 1.56 1.60 1.54 1.36 0.93 1.44 1.46 1.28 1.61 0.00 HuongTichSonNo1 1.27 1.45 1.01 1.01 0.95 0.59 1.25 0.75 1.25 1.27 0.77 0.73 1.32 0.00 QueLuc 1.21 1.10 0.86 0.86 0.81 0.93 1.33 1.23 1.19 1.77 0.56 0.67 1.77 0.75 0.00 VanNam 0.67 0.69 0.77 0.95 0.81 1.04 1.08 0.98 0.83 1.08 0.63 0.74 0.79 1.16 0.88 0.00 ThamVe 1.91 1.43 1.50 0.00 2.05 1.15 1.34 0.73 1.60 1.40 1.45 1.55 1.63 1.30 1.94 1.24 0.00 ChatTien 0.85 1.23 0.95 1.18 0.99 1.04 1.08 1.23 1.34 1.55 1.00 0.82 1.49 1.16 1.10 1.23 1.24 0.00 CuDePhung 1.34 1.55 1.85 1.85 1.66 2.12 1.17 1.55 1.50 1.34 0.98 1.09 1.46 1.61 1.37 1.37 1.07 0.85 0.00 GiaVai 1.10 1.24 0.76 0.69 0.87 1.44 1.11 1.71 1.08 1.36 1.15 0.89 1.92 0.98 1.02 1.53 1.57 0.55 0.74 0.00 LungPhin 0.97 0.99 1.17 1.17 1.10 1.27 1.19 1.50 1.64 1.88 1.11 1.02 2.98 1.56 1.09 1.34 1.35 0.73 0.70 0.79 0.00 NamNgat 1.27 1.04 1.24 1.52 1.74 1.50 0.93 1.63 1.40 1.61 1.31 1.06 2.24 1.91 1.45 0.93 1.30 1.85 0.91 1.08 1.15 0.00 NamTy 0.63 1.21 1.17 1.29 0.92 1.27 0.75 1.34 1.64 1.32 0.91 0.93 1.60 1.40 1.34 0.99 1.35 0.99 1.07 1.13 1.00 1.27 0.00 B'Lao 0.90 0.92 1.00 1.10 1.13 0.98 0.64 1.94 1.89 1.51 0.86 0.89 3.02 1.18 0.92 1.38 1.79 1.02 1.10 0.83 0.87 0.82 1.04 0.00 MinhRongNo1 1.21 1.23 1.30 1.08 1.12 1.29 1.00 1.51 2.16 1.90 1.25 1.04 1.61 1.29 0.91 1.11 1.78 0.82 1.09 0.81 0.93 1.42 1.02 0.81 MinhRongNo3 1.07 1.21 1.17 1.06 1.00 1.56 1.08 1.68 1.17 1.48 1.11 1.02 2.29 1.40 1.09 1.34 1.76 1.09 1.07 0.87 1.00 1.40 1.00 1.04 TB14 0.82 1.38 1.10 0.83 0.79 1.44 0.71 1.71 1.89 1.51 1.40 1.15 1.92 1.59 1.38 1.38 2.08 1.02 1.51 0.90 1.04 1.30 0.73 0.69 ShanTanChi 0.93 1.06 0.69 0.85 0.73 1.24 0.87 1.65 1.01 1.15 0.79 0.82 1.34 1.24 1.06 0.95 2.01 1.18 1.15 0.83 1.17 1.24 0.88 0.91 ToHieu 1.32 1.75 1.27 0.91 0.85 1.11 0.74 1.53 1.48 2.02 1.21 1.18 2.83 1.40 1.19 2.44 2.30 1.19 1.50 1.08 1.05 2.10 1.30 1.20 HoocMon 1.14 1.32 1.24 1.40 1.28 1.09 1.26 1.17 1.28 1.48 1.34 1.29 1.42 1.38 1.17 0.91 1.58 0.81 1.70 1.18 1.43 1.56 2.53 1.18 1A 1.01 1.18 1.67 1.44 1.48 1.09 0.99 1.03 2.24 1.86 1.39 1.31 1.06 1.09 1.59 1.37 1.22 1.03 1.35 1.51 1.48 1.65 1.14 1.17 5A 1.06 1.55 0.93 1.04 0.97 1.27 0.85 1.21 1.50 1.75 1.23 1.09 1.46 1.14 1.77 1.77 1.91 0.85 1.06 0.74 1.32 2.12 1.32 1.22 F16 1.00 1.43 1.22 1.35 1.13 1.49 0.92 1.27 1.38 1.12 0.94 1.15 2.22 1.20 1.14 1.14 1.47 1.27 1.12 1.06 1.25 1.33 1.13 1.17 F35 0.90 1.24 0.91 0.69 0.73 0.90 0.85 1.24 1.33 1.69 0.70 0.68 1.41 0.90 0.76 0.84 1.57 0.76 1.22 0.83 0.95 1.08 0.87 0.90 LCT 1.57 1.59 2.07 2.07 2.11 1.65 2.78 1.99 1.55 1.57 2.71 1.72 2.38 1.65 1.99 2.69 2.54 1.08 1.57 1.04 1.01 2.75 2.11 1.45 LDPNo2 1.97 0.00 1.67 1.38 1.70 1.65 1.40 1.99 1.95 2.67 2.02 1.21 1.69 2.05 1.30 1.59 2.54 2.69 1.28 1.22 0.00 1.14 2.11 1.45 PHNo1 0.87 1.14 1.22 0.97 0.90 1.20 0.98 1.48 0.85 0.99 1.32 1.27 1.39 1.35 1.48 1.01 2.94 1.14 0.99 0.83 1.00 1.20 0.90 1.15 PHT 1.12 1.14 1.37 1.56 1.25 1.35 1.10 1.99 1.95 1.97 0.71 1.14 1.39 1.54 0.89 0.89 1.33 0.78 1.12 1.03 1.41 1.54 1.12 0.83 S28 0.91 1.16 1.24 0.83 0.78 1.22 1.03 1.29 1.40 1.42 0.96 1.17 1.54 1.22 1.29 1.29 1.48 0.84 1.27 0.74 0.95 1.50 1.05 0.98 TrungDuThanhDuc 0.89 1.27 0.81 0.90 0.93 1.20 1.12 1.43 1.23 1.81 0.75 0.78 1.12 1.08 1.02 0.82 1.69 1.02 1.59 1.28 1.25 1.49 0.85 1.42 TrungDuTim 0.86 1.45 1.12 1.30 1.33 1.79 1.54 0.89 1.18 1.20 1.24 1.35 0.73 1.50 2.14 0.89 1.30 1.45 1.20 1.59 0.00 1.79 1.33 2.98 TrungDuVang 1.60 1.40 1.14 1.14 1.17 0.99 0.80 0.93 2.27 1.89 1.23 1.05 0.75 1.12 1.06 0.93 1.07 1.62 2.29 1.54 2.02 1.12 1.33 1.36 TrungDuXanh 1.22 0.96 1.03 1.17 1.35 1.30 1.34 1.09 1.38 1.22 0.86 0.97 1.12 1.15 1.09 0.84 1.50 1.43 1.63 1.79 1.54 1.48 1.35 1.79 TrungDuXanhHonHop 0.79 0.91 0.89 0.70 0.74 1.22 0.72 1.17 1.28 1.30 1.05 1.04 1.42 0.97 1.32 0.91 2.27 1.17 1.48 0.95 1.14 1.09 0.74 1.31 BanXenNo2 2.67 1.99 2.07 2.77 2.11 1.36 2.09 0.90 1.55 1.97 2.71 1.43 1.28 1.65 1.30 1.59 0.93 1.30 1.28 1.22 2.11 2.05 2.11 1.73 MocChau 1.22 0.84 0.83 0.69 0.79 1.77 1.22 1.38 1.33 1.51 1.15 0.97 1.92 1.30 1.02 1.13 2.08 1.24 1.51 0.90 1.24 1.18 1.49 1.07 SuoiGiang 0.73 0.93 0.75 1.01 0.78 0.90 1.39 1.16 1.25 1.61 0.77 0.59 1.54 0.90 1.04 0.60 1.01 0.84 1.14 0.90 0.78 0.90 1.05 0.90 ShanTuyetMocChau 0.67 0.62 0.77 0.93 1.06 1.12 1.05 0.95 1.13 1.29 0.97 0.90 1.56 1.12 0.69 0.69 1.17 1.18 1.44 1.20 1.06 0.92 1.17 0.83 M in hR on gN o1 M in hR on gN o3 TB 14 Sh an Ta nC hi To H ie u H oo cM on 1A 5A F1 6 F3 5 LC T LD PN o2 PH N o1 PH T S2 8 Tr un gD uT ha nh D uc Tr un gD uT im Tr un gD uV an g Tr un gD uX an h Tr un gD uX an hH on H op Ba nX en N o2 M oc Ch au Su oi G ia ng Sh an Tu ye tM oc Ch au MinhRongNo1 0.00 MinhRongNo3 0.85 0.00 TB14 0.45 0.95 0.00 ShanTanChi 0.74 0.59 0.83 0.00 ToHieu 1.07 1.17 0.98 1.13 0.00 HoocMon 1.04 1.14 1.46 1.40 1.46 0.00 1A 1.02 1.99 1.33 1.44 0.99 0.96 0.00 5A 0.98 0.97 1.00 0.84 0.92 1.30 1.01 0.00 F16 1.27 0.93 1.17 0.99 0.98 1.21 1.41 0.80 0.00 F35 0.68 0.95 0.83 0.76 0.88 1.18 1.04 1.10 1.06 0.00 LCT 1.21 1.41 1.73 2.07 1.55 1.93 1.79 1.28 1.34 2.14 0.00 LDPNo2 1.43 1.70 1.45 1.67 1.95 1.93 1.79 1.97 1.63 1.22 2.20 0.00 PHNo1 0.91 0.90 1.15 0.68 1.10 1.41 1.28 0.99 1.34 1.03 0.65 1.90 0.00 PHT 0.82 1.12 1.03 1.22 1.66 1.07 1.09 1.68 0.93 0.93 2.60 1.50 1.39 0.00 S28 0.80 1.05 0.82 1.12 0.80 1.38 0.73 1.02 1.33 0.61 1.36 1.65 0.95 0.95 0.00 TrungDuThanhDuc 1.15 1.25 0.96 1.10 1.10 1.77 1.22 1.25 1.47 0.72 2.04 1.63 1.34 0.93 0.97 0.00 TrungDuTim 1.86 1.84 1.88 1.30 2.10 1.67 1.02 1.42 1.26 1.18 2.34 1.24 1.64 1.35 1.28 0.93 0.00 TrungDuVang 0.83 1.33 0.95 0.78 1.58 0.99 0.86 1.04 1.25 1.07 0.00 1.13 1.53 0.97 1.27 1.10 1.05 0.00 TrungDuXanh 1.55 2.05 1.79 1.50 2.99 1.87 1.73 1.91 1.69 1.23 2.54 2.54 2.25 1.56 1.48 1.13 1.59 1.07 0.00 TrungDuXanhHonHop 1.04 0.92 0.86 0.89 0.87 1.66 1.12 1.01 0.85 0.77 1.52 1.52 0.72 1.23 0.77 0.95 1.16 0.86 1.36 0.00 BanXenNo2 1.43 1.70 2.83 2.07 1.95 1.52 1.39 1.28 2.04 2.83 1.10 1.50 1.21 1.90 2.05 2.04 1.65 1.42 2.54 0.00 0.00 MocChau 0.97 1.13 1.16 1.10 1.08 1.31 1.04 1.22 1.28 0.98 2.14 1.45 1.15 1.15 0.74 1.06 1.37 0.95 1.10 0.77 1.45 0.00 SuoiGiang 0.80 1.15 1.08 0.92 1.40 0.87 1.09 1.42 1.49 0.74 2.05 1.65 1.35 0.95 0.99 0.71 1.28 0.99 1.15 1.38 1.65 1.08 0.00 ShanTuyetMocChau 1.08 1.17 1.10 0.93 1.27 0.79 0.75 1.15 0.73 1.00 2.07 1.38 1.22 0.86 1.01 1.22 1.30 0.78 1.03 0.89 1.38 0.69 0.75 0.00 i i am o Bảng 1.1/3 A CT 49 A CT 63 A ss am D aj el lin g M an ip ur D an gr i M an ip ur M es sa i Sw in gl ay ba ri Li ne 27 Li ne 29 Ki m Tu ye n N go cT hu y O Lo ng Th an hT am G ru iz ia 16 G ru iz ia 19 62 G ru iz ia 2 G ru iz ia 4 G ru iz ia 5 G ru iz ia 8 Ko nK hi tD a N ha tB an La N ho H an Q uo cN o3 H an Q uo cN o1 H an Q uo cN o2 M ac om en Po us an g Je tin ga Ka na ya M id or y M er iy ok u M id or i O ku hi ka ri O ku m id i O ku yu ta ka Sa em id or y 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 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0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Tóm tắt luận văn  Chè (Camellia sinensis (L.) O. Kuntze) là loại cây trồng có giá trị  trong phát  triển kinh  tế nông nghiệp vùng  trung du và miền núi.  Chương  trình  lai  tạo giống chè ở Việt Nam cho đến này vẫn được  tiến hành theo phương pháp lai, chọn tạo truyền thống đạt hiệu quả  không cao. Nhằm hỗ trợ công tác lai tạo thông qua chọn lựa cặp bố  mẹ phù hợp và tạo cơ sở ban đầu cho công tác bảo tồn các giống chè  ở Việt Nam, chúng tôi đã tiến hành đề tài “Nghiên cứu đánh giá sự  đa dạng di  truyền  các  giống/dòng  chè  (Camellia  sinensis  (L.) O.  Kuntze  ở  Việt Nam  bằng  chỉ  thị  hình  thái  và  chỉ  thị  phân  tử  microsatellite (SSR)”.   Sử dụng kết hợp hai loại chỉ thị hình thái và chỉ thị SSR, đề tài  đã  đánh  giá  sự  đa  dạng  di  truyền  của  96  giống/dòng  chè  từ  các  nguồn khác nhau  (giống địa phương, giống nhập nội và giống  lai‐ chọn tạo) đang được bảo tồn tại vườn quỹ gen chè, Viện Khoa học  Nông Lâm nghiệp miền núi phia Bắc (NOMAFSI). Kết quả cho thấy  các giống/dòng chè nghiên cứu được phân nhóm chính xác vào các  thứ chè Assam, chè Trung Quốc, chè Cambod và có sự đa dạng di  truyền rất lớn. Bên cạnh đó, thông qua số liệu quan hệ di truyền và  kết quả phân nhóm, đề tài cũng phát hiện đặc điểm cùng nguồn gốc  của  nhiều  giống  chè  như  các  giống  chè Nhật  Bản,  các  giống  chè  Shan Việt Nam hay các dòng chè lai. Kết quả nghiên cứu của đề tài  không chỉ giúp các nhà chọn giống lựa chọn cây bố mẹ phù hợp cho  phép  lai mà còn giúp đánh giá,  sàng  lọc các giống/dòng chè đang  được bảo tồn, qua đó nâng cao hiệu quả gìn giữ và khai thác nguồn  gen chè.  Từ khóa: Camellia sinensis, đa dạng di truyền, chỉ thị hình thái, SSR,  NTSYS.