Sau 5 năm nghiên cứu và thực hiện, Luận án đã xây dựng được quy trình nghiên
cứu triển khai công nghệ khí hóa sinh học than ngầm quy mô phòng thí nghiệm từ
quá trình lấy mẫu, bảo quản và gia công phân tích mẫu cho đến xác lập các điều
kiện thí nghiệm phù hợp với đối tượng là than Sông Hồng và quần xã vi sinh vật
bản địa kị khí nghiêm ngặt. Kết quả của Luận án là cơ sở khoa học để triển khai
công nghệ khí hóa sinh học than ngầm tại bể than Sông Hồng, Việt Nam. Các kết
quả nghiên cứu chính đã hoàn thành được hai mục tiêu đề ra trong Luận án, được
trình bày dưới đây:
1. Các đặc điểm địa chất và hóa địa thủy văn của bể than Sông Hồng tại khu
vực nghiên cứu khẳng định tính khả dụng sinh học để khí hóa than ngầm bằng vi
sinh vật: tồn tại 05 vỉa than tương ứng 5 vị trí lấy mẫu (C1 đến C5; chiều sâu vỉa từ
396,04 m đến 853,82 m) có bề dày vỉa khoảng từ 3 ̶ 5 m; các vỉa than có thành phần
chất bốc cao (40,29 ̶ 47,19%); kích thước lỗ rỗng phù hợp cho vi sinh vật xâm nhập
trong than (> 400 nm, chiếm 11,98 ̶ 36,08% tổng thể tích lỗ) cùng tính liên thông lỗ
rỗng tốt; nước ngầm liên kết bể than mang đặc trưng của một bể than có khả năng
sinh khí.
2. Đã chứng minh được sự tồn tại của quần xã vi sinh vật bản địa có khả năng
chuyển hóa than thành khí trong bể than sông Hồng tại khu vực nghiên cứu. Ngành
Proteobacteria chiếm ưu thế trong các vỉa than, với chi phổ biến nhất thuộc
Burkholderia-Caballeronia-Paraburkholderia. Các ngành Proteobacteria –
Archaea – Firmicutes – Actinobacteria – Bacteroidete là các ngành phổ biến theo
mức độ giảm dần trong mẫu nước liên kết bể than, với chi phổ biến nhất là
Shewanella. Con đường chuyển hóa sinh khí methane dinh dưỡng methyl với nhóm
cổ khuẩn Methanolobus chiếm ưu thế. Quần xã vi sinh vật bản địa đáp ứng với việc
bổ sung dinh dưỡng, cho hiệu suất sinh khí cao hơn trong thời gian ngắn hơn, dao
động từ 1,14 đến 4,96 mL CH4/g than.
Bằng các nghiên cứu đã nêu trong Luận án, đã xác định được tính khả thi và
phạm vi áp dụng phù hợp của giải pháp khí hóa sinh học than ngầm trong bể than
Sông Hồng tại vùng nghiên cứu là các vỉa than có chiều sâu vỉa tới 745,25 m.
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