Luận án Nghiên cứu khả năng khí hóa than của hệ vi sinh vật từ bể than sông Hồng

Sau 5 năm nghiên cứu và thực hiện, Luận án đã xây dựng được quy trình nghiên cứu triển khai công nghệ khí hóa sinh học than ngầm quy mô phòng thí nghiệm từ quá trình lấy mẫu, bảo quản và gia công phân tích mẫu cho đến xác lập các điều kiện thí nghiệm phù hợp với đối tượng là than Sông Hồng và quần xã vi sinh vật bản địa kị khí nghiêm ngặt. Kết quả của Luận án là cơ sở khoa học để triển khai công nghệ khí hóa sinh học than ngầm tại bể than Sông Hồng, Việt Nam. Các kết quả nghiên cứu chính đã hoàn thành được hai mục tiêu đề ra trong Luận án, được trình bày dưới đây: 1. Các đặc điểm địa chất và hóa địa thủy văn của bể than Sông Hồng tại khu vực nghiên cứu khẳng định tính khả dụng sinh học để khí hóa than ngầm bằng vi sinh vật: tồn tại 05 vỉa than tương ứng 5 vị trí lấy mẫu (C1 đến C5; chiều sâu vỉa từ 396,04 m đến 853,82 m) có bề dày vỉa khoảng từ 3 ̶ 5 m; các vỉa than có thành phần chất bốc cao (40,29 ̶ 47,19%); kích thước lỗ rỗng phù hợp cho vi sinh vật xâm nhập trong than (> 400 nm, chiếm 11,98 ̶ 36,08% tổng thể tích lỗ) cùng tính liên thông lỗ rỗng tốt; nước ngầm liên kết bể than mang đặc trưng của một bể than có khả năng sinh khí. 2. Đã chứng minh được sự tồn tại của quần xã vi sinh vật bản địa có khả năng chuyển hóa than thành khí trong bể than sông Hồng tại khu vực nghiên cứu. Ngành Proteobacteria chiếm ưu thế trong các vỉa than, với chi phổ biến nhất thuộc Burkholderia-Caballeronia-Paraburkholderia. Các ngành Proteobacteria – Archaea – Firmicutes – Actinobacteria – Bacteroidete là các ngành phổ biến theo mức độ giảm dần trong mẫu nước liên kết bể than, với chi phổ biến nhất là Shewanella. Con đường chuyển hóa sinh khí methane dinh dưỡng methyl với nhóm cổ khuẩn Methanolobus chiếm ưu thế. Quần xã vi sinh vật bản địa đáp ứng với việc bổ sung dinh dưỡng, cho hiệu suất sinh khí cao hơn trong thời gian ngắn hơn, dao động từ 1,14 đến 4,96 mL CH4/g than. Bằng các nghiên cứu đã nêu trong Luận án, đã xác định được tính khả thi và phạm vi áp dụng phù hợp của giải pháp khí hóa sinh học than ngầm trong bể than Sông Hồng tại vùng nghiên cứu là các vỉa than có chiều sâu vỉa tới 745,25 m.

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