Luận án Nghiên cứu tuyển chọn chủng nấm men để ứng dụng trong sản xuất whisky từ malt đại mạch và ngô

ang Thảo, Nguy n Thành ạt, Nguy n Quang ào (1999), M t số kết quả lên men vang từ quả vải thiều (Litchi chinensis sonn), áo cáo khoa học, i nghị ông nghệ sinh học quốc gia 16. Nguy n Minh Thu và c ng sự (2016), Nghiên cứu công nghệ sản xuất rượu whisky từ malt đại mạch và nguyên liệu thay thế của Việt Nam, ề án ứng dụng và phát triển công nghệ sinh học trong lĩnh vực công nghiệp chế biến đến năm 2020, M số: T.07.12/ NS 17. Trần Linh Thước và TV (2002), Tạo d ng nấm men S. serevisiae tái tổ hợp biểu hiện gen m h a glucoamylase. Tạp chí Phát triển Khoa học công nghệ ại học Quốc gia TP. M, Tập 5, số 7, tr 31 – 43. 18. Tiêu chu n Việt Nam T VN 3217:1979 về rượu - Phân tích cảm quan - Phương pháp cho điểm 19. Tiêu chu n Việt Nam T VN 378-86 Rượu trắng, phương pháp thử 20. Tiêu chu n quốc gia T VN 8764:2012 Thức ăn chăn nuôi - phương pháp xác định axit amin 21. Tiêu chu n quốc gia T VN 7864:2013 thanol nhiên liệu biến tính - xác định hàm lượng ethanol - phương pháp sắc ký khí 22. Tiêu chu n quốc gia T VN 8009:2009 rượu chưng cất - xác định hàm lượng aldehyt 23. Tiêu chu n quốc gia T VN T VN 8010: 2009 rượu chưng cất - xác định hàm lượng metanol 24. Dương á Toàn (2015), Phương pháp làm giá đ . NX Phương ông, trang: 5 - 8 25. Tổng công ty ia - Rượu - Nước giải khát Việt Nam (1998), hiến lược phát triển khoa học công nghệ ngành rượu bia nước giải khát đến 2020 và l trình công nghệ đến năm 2025. 26. Viện dinh dưỡng, y tế (2007), ảng thành phần thực ph m Việt Nam. NX Y học, tr 8, 19, 59, 118 148 II. T i liệu t m k o tiến n 27. Acharya D., & Adhikari B. (2014), Study on Use of Naked Barley (Hordeum vulgare var. Solu Uwa) Malt and Mold Bran as Enzyme Source for Preparation of Whisky. Journal of Food Science and Technology Nepal, 7, 44-51. 28. Agu R. C., Bringhurst T. A., and Brosnan J. M. (2006), Production of grain whisky and ethanol from wheat, maize and other cereals. J. Inst. Brewing 112, 314–323. 29. Alcarde A. R., Souza L. M., and Bortoletto A. M. (2014), Formation of volatile and maturation-related congeners during the aging of sugarcane spirit in oak barrels. J. Inst. Brewing 120, 529–536. 30. Amerine M.A, and Cruess M. V., (1960), The technology of wine making. The Avi Publishing Company, Inc. (2, 3, 144-150, 163-203). 31. Ancín C., Garde T., Torrea D., Jiménez N (2004), Extraction of volatile compounds in model wine from different oak woods. Food Research International, 37. 375-383. 32. Anna Maráz (2011), Food fermentations by yeasts and filamentous fungi, Department of Microbiology and Biotechnology Faculty of Food Science Corvinus University of Budapest Erasmus IP Maribor. 33. António M. Jordão, Jorge M. Ricardo da Silva, Olga Laureano, An Adam, Jan Demyttenaere. (2006), Volatile composition analysis by solid-phase microextraction applied to oak wood used in cooperage: effect of botanical species and toasting process. Journal of Wood Science, 52. 514-521. 34. Arroyo-López FN, Durán Quintana MC, Garrido Fernández A (2006), Use of the generalized z-value concept to study the effects of temperature, NaCl concentration and pH on Pichia anomala, a yeast related to table olive fermentation. Int J Food Microbiol 106: 45–51. 35. Ashok Pandey et al (2000), Advances in microbial amylases. Biotechnol. Appl. Biochem. 31, 135–152 (Printed in Great Britain) 36. Barnet. J. A., Payne. R. W, Yarrow. D., (1990), Yeast characteristic and identification. Cambridge University Press. (5-10). 37. Barry M. Harrison, Fergus G. Priest (2009), Composition of Peats Used in the Preparation of Malt for Scotch Whisky Production—Influence of Geographical Source and Extraction Depth. J. Agric. Food Chem.2009,57,6, pp 2385-2391 38. Barry M, Harrison, Joanne Ellis, David Broadhurst, Ken Reid, Royston Goodacre (2006), Differentiation of Peats Used in the Preparation of Malt for Scotch Whisky Production Using Fourier Transform Infrared Spectroscopy. Journal of The Institute of Brewy. Volume112, Issue 4, Pages 333-339 149 39. Benoit Divol, Maret du Toit, Edward Duckitt (2016), Surviving in the presence of sulphur dioxide: strategies developed by wine yeasts. Applied Microbiology and Biotechnology, August 2012, Volume 95, Issue 3, pp 601–613 40. Berry D. R., Brown C. (1987), Physiology of growth. Yeast Biotechnology, 159–199. 41. Berry D.R (1984), Physiology and microbiology of Scotch whisky production. In Progress in Industrial Microbiology; Bushell, M.E., Ed.; Elsevier: Amsterdam, The Netherlands; Volume 19, pp. 199 – 243 42. Brígida Fernández de Simón, Estrella Cadahía, Teresa Hernández, Isabel Estrella (2006), Evolution of oak-related volatile compounds in Spanish red wine during 2 years bottled, after aging in barerls made of Spanish, French and American oak wood. Journal of Analytica Chimica Acta, 563. 198-203. 43. ringhurst T. A., and rosnan J. (2014), ―Scotch whisky: raw material selection and processing,‖ in Whiskey Technology, Production and Marketing, 2nd Edn., eds I. Russel and G. Stewart (Boston, MA: Academic Press/Elsevier) 49–122. 44. Brock (2014), Biology of microorganisms. 14th ed, 2014, pp 126 -135. 45. Caio PupinRosa, Gustavo AndradeBrancaglion, Tatimara M.Miyauchi-Tavares, Patrícia PaivaCorsetti, Leonardo Augustode Almeida (2018), Antibiotic- induced dysbiosis effects on the murine gastrointestinal tract and their systemic repercussions. Life Sciences, Volume 207, 15 August 2018, Pages 480-491 46. Camara J. S, Marques J. C, Perestrelo R. M, Rodrigues F (2002), Comparision study of the whisky aroma profile based on headspace solid phase microextration using different, pp 123 – 136. 47. Campbell, I., (2003), Yeast and Fermentation, in Whisky Technology, Production and Marketing, edited by I Russell, Academic Press, London UK, pp117-153 48. Carla Da Porto, Sabrina Moret (2007), Comparision of polycyclic aromatichydrocarbons (PAHs) between smoked marc spirit and whiskies. Food and Chemical Toxicology, 45. 2069-2071. 49. Cerdán T. G., Rodriguez Mozaz S., and Ancin Azpilicueta C. (2002), Volatile composition of aged wine in used barrels of French oak and of American oak. Food Res. Int. 35, 603–610. 50. Coldea TE, Mudura E, Sibotean C, Coma E (2014), The brewing process: optimizing the Fermentation. Bull UASVM Food Sci Technol 71: 2–5. 150 51. Collins T. S., Zweigenbaum J., and Ebeler S. E. (2014), Profiling of non- volatiles in whiskeys using ultra high pressure liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC–QTOF MS). Food Chem. 163, 186–196. 52. Conner J. M., Paterson A., and Piggott J. R. (1993), Changes in wood extractives from oak cask staves through maturation of Scotch malt whisky. J. Sci. Food Agric. 62, 169–174. 53. Da Porto, C., Moret, S., Soldera, S.A (2006), Study on the composition of distillates obtained from smoked marc. Analysis of Chemistry, 563. 396-400. 54. D'Amato D, Corbo MR, Del Nobile MA, Sinigaglia M (2006), Effects of temperature, ammonium and glucose concentrations on yeast growth in a model wine system.Int J Food Sci Technol 41: 1152–1157. 55. David I. G. Green, Reginald C. Agu, Thomas A. Bringhurst, James M. Brosnan, Frances R. Jack and Graeme, Walker M (2015), Maximizing alcohol yields from wheat andmaize and their co-products for distilling orbioethanol production. Wiley Online Library: 121, 332 – 337 56. Deacon J (2006), Fungal Biology, 4th edition. Institute of Cell and Molecular Biology, University of Edinburgh, UK. Blackwell Publishing, pp 3-5, 53-59, 76-83, 145-156. 57. Falguni, Patra, AmiPatel, NihirShah (2017), Microbial Production of Food Ingredients and Additives, Handbook of Food Bioengineering. Pages 259-290. 58. Forsberg H, Ljungdahl PO (2001), Genetic and biochemical analysis of the yeast plasma membrane Ssy1p-Ptr3p-Ssy5p sensor of extracellular amino acids. Mol Cell Biol 21:814–826. 59. Fujio Y, Ogata M, Ueda, (1985), Etanol fermentatiom by energey saving process. J. Ferment Technol. 60. Gaiser M, Bell G. M, Lim A. W, Roberts N. A, Faraday D. B. F, Schulz R. A and Grob R (2002), Computer simulation of a continuous whisky still. Journal of Food Engineering Volume 51, Issue 1, pp. 27-31. 61. Garcia J. S., Vaz B. G., Corilo Y. E., Ramires C. F., Saraiva S. A., Sanvido G. B., et al. (2013), Whisky analysis by electrospray ionization-fourier transform mass spectrometry. Food Res. Int. 51, 98–106. 62. Garde-Cerdán T, Lorenzo C, Carot J. M, Climent M. D, Salinas M. R (2010), Effects on composition, storage tim, geographic origin and oak type on the accumulation of some volatile oak compounds and ethyphenols in wines. Food Chemistry, 122. 1076-1082. 151 63. Gertien J, Smits C, Herman van den Ende and Frans M. Klis (2001), Differential regulation of cell wall biogenesis during growth and development in yeast. Microbiology (2001), 147, 781–794 64. Ghaly A. E, Kamal M, Avery A (2003), Influence of temperature rise on kinetic parameters during batch propagation of Kluyveromyces fragilis in cheese whey under ambient conditions. World Journal of Microbiology and Biotechnology. Volume 19, Issue 7, pp 741–749. 65. Graeme Walker and Graham G Stewart (2016), Saccharomyces cerevisiae in the Production of Fermented Beverages. Academic Editor: Edgar Chambers IV, Received: 20 October; Accepted: 11 November 2016; Published: 17 November 2016. Pages 6 - 8 66. Graeme Walker, James Brosnan, Tom Bringhurst and Frances Jack (2012), Selecting new distilling yeasts for improved fermentation and for sustainability. Yeast Research Group, School of Contemporary Sciences, University of Abertay Dundee, Dundee DD1 1HG; Scotch Whisky Research Institute, Research Avenue North, Riccarton, Edinburgh, EH14 4AP. Scientific conference, Chapter 6, pages 1-11. 67. Graham Stewart (2002), American distiller: A source of information on the distilling process. Distilling. Scotch Whisky Production vs. the Brewing Process, Vol.1, No.3, pp. 52 - 61. 68. Guo ZP, Zhang L, Ding ZY, Wang ZX and Shi GY (2009), Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Applied Microbiology and Biotechnology 82: 287-292 69. Hans-GeorgSchmarr, MaximilianMathes, KristinaWall, FrankMetzner, MariusFraefel (2017), Enantiodifferentiation of whisky and cognac lactones using gas chromatography with different cyclodextrin chiral stationary phases. ELSEVIER. Journal of Chromatography A, Volume 1516, 22 September, Pages 135-141. 70. Helena Albergaria, Nils Arneborg (2016), Dominance of Saccharomyces cerevisiae in alcoholic fermentation processes: role of physiological fitness and microbial interactions. Applied Microbiology and Biotechnology, Volume 100, Issue 5, pp 2035–2046 (khả năng lên men cồn ưu thế) 71. Helena Albergaria. A, Gómez-Cordovés. M.C, Calderón. F, Suárez. J.A (2006), Effects of pH, temperature and SO2 on the formation of pyranoanthocyanins during red wine fermentation with two species of Saccharomyces. International Journal of Food Microbiology. Volume 106, Issue 2, 1 February 2006, Pages 123-129 152 72. Hiroshi Fukuhara (2003), The Kluyver effect revisited. FEMS Yeast Research, Volume 3, Issue 4, June 2003, Pages 327–331. 73. Holm Hansen. E, Nissen. P, Sommer. P, Nielsen. J.C (2001), The effect of oxygen on the survival of non‐Saccharomyces yeasts during mixed culture fermentations of grape juice with Saccharomyces cerevisiae. Applied Microbiology.Volume91, Issue3, Pages 541-547. 74. Iefuji H, Chino M, Kato M, Iimura Y (1996), Raw-starch-digesting and thermostable α-amylase from the yeast Cryptococcus sp. S-2: purification, characterization, cloning and sequencing. Biochem, 318: 989–996. 75. Inge Russell, Graham Stewart, Charlie Bamforth, Inge Russell (2003), Whisky Technology, Production and Marketing. Elsevier. 76. Ingledew W.M., (2009), Yeasts: Physiology, nutrition and ethanol production, in The Alcohol Textbook, Fifth Edition, edited by W.M. Ingledew, D.R. Kelsall, G,D. Austin and C. Kluhspies, Nottingham University Press, Nottingham, UK, pp101-113 77. Jack F.R (2012), Alcoholic Beverages Sensory Evaluation and Consumer Research Woodhead Publishing Series in Food Science, Technology and Nutrition. Pages 379-392. 78. Jan C.R., Demyttenaer, Jorge I. Sánchez Martínez, Roland Verhé, Pat Sandra and Norbert De Kimpe (2003), Analysis of volatiles of malt whisky by soid-phase microextraction and stir barsorptive extraxtion. Journal of Chromatography A. Volume 985, Issues 1-2, 24; p. 221-232 79. Janko Kaliterna, Ruud A. Weusthuis, Juan I. Castrillo, Johannes P. Van Dijken, Jack T. Pronk (1995), Transient responses of Candida utilis to oxygen limitation: Regulation of the Kluyver effect for maltose. Yeast, Volume 11, Issue 4, pp 317 – 325. 80. Jerusa S, Garcia, Boniek G, Vaz, Yuri E, Corilo, Christina F, Ramires (2013), Whisky analysis by electrospray ionization-Fourier transform mass spectrometry. Food Research International, 51, 98-106. 81. Joeline Xiberras, Mathias Klein, Elke Nevoigt (2019), Glycerol as a substrate for Saccharomyces cerevisiae based bioprocesses – Knowledge gaps regarding the central carbon catabolism of this ‗non-fermentable‘ carbon source. Biotechnology Advances 37 (2019) 107378, P 3 – 12. 82. José David Carrillo, María Teresa Tena (2006), Determination of volatile oak compounds in aged wines by multiple headspace solid-phase microextraction and gas chromatography-mas spectrometry (MHS-SHME- GC-MS). Anal Bioanal Chem, 385, 937-943. 153 83. Joshi B. H (2010), Purification and characterization of a novel protease from Bacillus firmus Tap 5 isolated from tannery industry, J. Appl. Sci. Res. 6 (2010) 1068 - 1076 84. Kreger Van Rij N.J.W., (1984), The yeast, a taxnomic study, 3rd ed. Elsevier, Amsterdam 85. Lawrence (1999), Using molecular markers to determine barleys most suitable for malt whisky distilling. Molecular Breeding. Volume 5, Number 2, 103-109. 86. Magasanik B, Kaiser CA (2002), Nitrogen regulation in Saccharomyces cerevisiae. Gene 290: P 1–18 87. Marco J.L., ataus L.A. & Valencina F.F (1996), Purification and characterization of a truncated B. subtilis α-amylase pro-duced by E. coli. Appl. Microbiol. Biotechnol. 44: 746–752. 88. Maria E.O, Mamedea Gláucia, Pastoreb. M (2006), Study of methods for the extraction of volatile compounds from fermented grape must. Food Chemistry, Volume 96, Issue 4, June 2006, Pages 586-590. 89. Maria Balcerek, Katarzyna Pielech-Przybylska, welina Strąk, Piotr Patelski, Urszula Dziekońska (2016), omparison of fermentation results and quality of the agricultural distillates obtained by application of commercial amylolytic preparations and cereal malts. European Food Research and Technology March 2016, Volume 242, Issue 3, pp 321–335. 90. Masahiro Endo, Kazutoh Takesako, Ikunoshin Kato, and Hideyo Yamaguchi (1997), Fungicidal Action of Aureobasidin A, a Cyclic Depsipeptide Antifungal Antibiotic, against Saccharomyces cerevisiae. Antimicrobial agents and chemotherapy, Mar. 1997, p. 672–676 91. Matia N. Lau, John D. Ebeler, Susan E. Ebeler (1999), "Gas Chromatographic Analysis of Aldehydes in Alcoholic Beverages Using a Cysteamine Derivatization Procedure", AJEV, US. Am J Enol Vitic. January 1999 50: 324-333 92. Meyhack B, Bajwa W, Rudolph H, and Hinnen A (1987), Two yeast acid phosphatase structural genes are the result of a tandem duplication and show different degrees of homology in their promoter and coding sequences, EMBO J. 1982; 1(6): 675–680. 93. Ming-Zhu Ding, Xin Wang, Yang Yang and Ying-Jin Yuan (2011), Metabolomic Study of Interactive Effects of Phenol, Furfural, and Acetic Acid on Saccharomyces cerevisiae. OMICS: A Journal of Integrative Biology.Oct. pp 35 – 42 154 94. MinZhou, YuandeLong, YonggangZhi, XiaoyingXu (2018), Preparation and chromatographic evaluation of a chiral stationary phase based on carboxymethyl-β-cyclodextrin for high-performance liquid chromatography. ELSEVIER. Chinese Chemical Letters, Volume 29, Issue 9, September 2018, Pages 1399-1403 95 Monties B (1987), Chemical composition of oak wood: phenolic compounds and their relation with physical and chemical properties related to quality of wines and spirits. Connaiss Vigne Vin, 22. 275-294. 96. Munro R (2008), Opportunities for improved yeast supplies in whisky and related spirits production, in: Distilled Spirits, Production, Technology and Innovation, edited by J.H. Bryce, J.R. Piggott, G.G. Stewart, Nottingham University Press, Nottingham UK, pp133-138 97. Nobuo Ochiai, Kikuo Sasamoto, Kenvin MacNamara (2012), Charaterization of sulfur compounds in whisky by full evaporation dynamic headspace and selectable one-dimensional/ two dimensional retention time element - specific detection. Journal of Chromatography A,1270. 296-304. 98. Noguchi Y., Urasaki K, Yomo H. and Yonezawa T., (2008), Effect on new- make spirit character due to performance of brewer‘s yeast – (II) various yeast strains containing commercial strains, in: Distilled Spirits, Production, Technology and Innovation, edited by J.H. Bryce, J.R. Piggott, G.G. Stewart, Nottingham University Press, Nottingham UK, pp110- 116. 99. Pereira F.B., Guimarães P. M. R, Teixeira J. A and Domingues L (2010), Optimization of lowcost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs. Bioresource Technology, 101: 7856-7863. 100. Peter H. Raver (2016), Biology 11th ed. 2016, pp 206 – 225. 101. Philp J. M (1989), Cask quality and warehouse conditions.The science and technology of whiskies, Longman Scientific and Technical, London, pp. 264-294. 102. Pickerell D. S (2007), Whisky Distillation. Encyclopedia of Separation Science, Loretto, KY, USA, p. 4485-4490. 103. Piggott J. R, Conner J. M, Paterson A (1999), Release of distillate flavour compounds in Scotch malt whisky. Wiley Online Library. 26 May, P 26 -35 104. Piggott J.R (2016), Current Developments in Biotechnology and Bioengineering. Food and Beverages Industry. Elsevier B.V, Amsterdam, Pages 435-450. 155 105. Piggott J. R, Conner J. M, Paterson A (1993), Effects on Scotch whisky composition and flavour of maturation in oak casks with varying histories. Wiley Online Library. June, Papes 13 – 21. 106. Piggott J. R, Conner J. M (1995),Whiskies. Fermented Beverage Production, pp 247-274 107. Piggott J.R, Anthony Hunter E, Lila Margomenou (2000), Comparision of methods of analysis of time-intensity data: application to Scotch malt whisky. Food Chemistry, 71. P 319-326. 108. Pisarnitskij Alecksandr Fomich, Macharashvili Gocha Iraklievic, Aliev Agil Aga Ragim Ogly (2007), Method for producing whiskey. Bibliographic data: RU2294365 (C1), pp 126 – 128. 109. Poison L., Schieberle P. (2008), Characterization of the most odor-active compounds in American bourbon whisky by application of the aroma extract diluteion analysis. Journal of Agricultural and Food Chemistry, 56. 5813-5819. 110. Pollnitz A. P, Jones GP, Sefton MA (1999), Determination of oak lactones in barrel-aged wines and in oak extracts by stable isotope dilution analysis. Journal of Chromatograph A, 875. P 239-246. 111. Querol A, Fleet G.H. (2006), The Yeast Handbook. Volume 1. Biodiversity and Ecophysiology of Yeasts. Volume 2. Yeasts in Food and Beverages; Springer: Berlin/Heidelberg, Germany, 2006, pp 231 – 256. 112. Ramsay C. M, Berry. D. R (1983), Development of a small scale mashing and fermentation system for studies on Malt Whisky production. Applied microbiology and Biotechnology. Vol. 8, No. 4, p. 207-213. 113. Rhodes C.N., Heaton K., Goodall I., Breteton P.A. (2009), Gas chromatography carbon isotope ratio mass spectrometry applied to the detection of neutral alcohol in Scotch whisky: An internal reference approach. Food Chemistry, 114. 697-701 114. Rose N.L (1990), A method for the extraction of carbonaceous particles from lake sediment. Journal of Paleolimnology. Volume 3, Issue 1, pp 45–53. 115. Russell I., Stewart G.G (2014), Whisky: Technology, Production and Marketing, 2nd ed.; Academic Press/Elsevier: Boston, MA, USA, 2014, pp 136 – 162. 116. Ruud A. Weusthuis, Wiebe Visser,t Jack T. Pronk, W. Alexander Scheffers and Johannes P. van Dijken (1994), Effects of oxygen limitation on sugar metabolism in yeasts: a continuous-culture study of the Kluyver effect. Microbiology, 140, 703-715 156 117. Sambrook J., Fritsch E. F., Maniatis T., (1989), Molecular Cloning, A Laboratory Manual, 2nd Edition, Cold Spring Harbor Laboratory Press, New York. 118. Schimz K-L, Holzer H (1979), Rapid decrease of ATP content in intact cells of Saccharomyces cerevisiae after incubation with low concentrations of sulfite. Arch Microbiol 121:225–229. 119. Sim G. B, Berry D. R (1996), Malted barley enzyme activity under optimum and process conditions from the scotch malt whisky industry. Enzyme and Microbial Technology, Volume 19, Issue 1, Pages 26-31. 120. Sivaramakrishnan S, et al (2006), Amylases from Microbial Sources, An Overview on Recent Developments, Food Technol. Biotechnol. 44 (2) 173– 184. ISSN 1330 – 9862 121. Stuart Swanston J, William T.B. Thomas, Wayne Powell, George R. Young and Patricia E. Lawrence (1999), Using molecular markers to determine barleys most suitable for malt whisky distilling. Molecular Breeding, 5. P 103-109. 122. Szambelan K, Nowak J, Czarnecki Z (2004), Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers, Biotechnol Lett, No 26(10):845-80. 123. Teresa Garde Cerdán, Casndida Lorenzo, Gonzalo L. Alonso, M. Rosario Salinas (2010), Employment of near infrared spectrocopy to determine oak volatile compounds and ethyphenols in aged red wines. Food Chemistry, 119. 823-828. 124. Thomas A, all (1999), ― ioedit: a user friendly biological sequence alignment editor and analysis program for windows 95/98/NT‖, Oxford University Press, Nucleic acids symposium series No.41, pp. 95-98. 125. Torija MJ, Rozès N, Poblet M, GuillamónJM, Mas A (2003), Effects of fermentation temperature on the strain population of Saccharomyces cerevisiae. Int J Food Microbiol 80: 47–53. 126. Walker G.M., Stewart G.G (2016), Saccharomyces cerevisiae in the Production of Fermented Beverages. Beverages, p 2, 30. 127. Walker G.M., Hill A.E (2016), Saccharomyces cerevisiae in the Production of Whisk(e)y. Beverages 2, 38. pp 2 - 15 128. Walker G.M (2014), Microbiology of wine-making. In Encyclopedia of Food Microbiology; Batt, C., Tortorello, M.L., Eds.; Elsevier Science Publishers: Boston, MA, USA, 2014; pp. 787–792. 157 129. Walker G.M (2014), Fermentation (Industrial). Media for Industrial Fermentations. In Encyclopedia of Food Microbiology; Batt, C., Tortorello, M.L., Eds.; Elsevier Science Publishers: Boston, MA, USA, pp 162 – 198. 130. Wang Z.X., J. Zhuge H. Fang and Prior B. A (2001), Glycerol production by microbial fermentation: A review. Biotechnology Advances. 19: 201-223. 131. Watson, DC (1981), The development of specialised yeast strains for use in Scotch whisky fermentations. In: Current Developments in Yeast Research. Advances in Biotechnology. Eds. GG Stewart and I Russell. Pergamon Press, Oxford, pp 57-62. 132. Watson, D.C (1984), Distilling Yeasts, Developments in Industrial Microbiology, 25, 213-220. 133. Watson E.B, Othman DB, Luck JM, Hofmann AW (1987), Partitioning of U, Pb, Cs, Yb, Hf, Re and Os between chromian diopsidic pyroxene and haplobasaltic liquid. Chemical Geology, Volume 62, Issues 3–4, Pages 191-208 134. Will Kew, Ian oodall, David larke, Dušan Uhrín (2017), hemical Diversity and Complexity of Scotch Whisky as Revealed by High- Resolution Mass Spectrometry. Journal of The American Society for Mass Spectrometry January, Volume 28, Issue 1, pp 200–213. 135. Xingyan Liu, Bo Jia, Xiangyu Sun, Jingya Ai, Cheng Wang, Fang Zhao, Jicheng Zhan, Weidong Huang (2015), Effect of Initial PH on Growth Characteristics and Fermentation Properties of Saccharomyces cerevisiae. Food Science. Volume80, Issue4, pp800 - 808 136. Yaya Liu, Anissa el Masoudi, Jack T. Pronk, Walter M. van Gulik (2019), Quantitative physiology of non-energy-limited retentostat cultures of Saccharomyces cerevisiae at near-zero specific growth rates. It is made available under a CC-BY-NC-ND 4.0 International license 137. Yin-Won Lee1 (2011), Fungi Associated with the Traditional Starter Cultures Used for Rice Wine in Korea, Dong-A University, J. Korean Soc. Appl. Biol. Chem. 54(6), 933- 943. 138. Zhang W, Du G, Zhou J, Chen J (2018), Regulation of sensing, transportation, and catabolism of nitrogen sources in Saccharomyces cerevisiae. Microbiol Mol Biol, Volume 82 Issue 1. P 2 – 19. 158 III. T i liệu t m k o tr n Internet 139. abed4070d3, ngày truy cập 10/9/2019. 140. https://www.businessinsider.com/the-biggest-whiskey-market-in-the-world- 2015-6, ngày truy cập 23/8/2019. 141. https://www.grandviewresearch.com/industry-analysis/travel-retail-market, ngày truy cập 28/8/2019. 142. https://tapchidouong.com.vn/nganh-bia-ruou-viet-nam-thuc-trang-san-xuat- kinh-doanh-va-su-dung-bia-ruou-1611.html, ngày truy cập 11/9/2019. 143. https://www.reportlinker.com/ci02016/Spirits.html, ngày truy cập 19/9/2019. 1PL 2PL PH C P ụ lục 1: T iết bị T thiết bị Nguồ gố PTN/ĐV - uồng đếm hồng cầu Thoma ức SP à N i - Máy đo đ cồn, chất hoà tan DA - 300 / KEM Density Nhật ản Cty Eresson - Máy đo p InoLab – WTW Nhật ản SP à N i - Máy ảnh Olympus 5050ZOOM Nhật ản SP à N i - Kính hiển vi điện tử Olympus X - 41 ức SP à N i - Kính hiển vi điện tử J M - T8 Nhật ản Viện VSDT TW - ân điện tử M – 200 Nhật SP à N i - Tủ sấy DKN - 811, Yamato Nhật ản SP à N i/ Cty Eresson - Nồi hấp SS325 - TOMY Mỹ SP à N i - Máy lắc SA - 32, Yamato Nhật SP à N i - Máy sắc ký khí System P 6890 series - t nhồi P - INNWax Polyethylene Glycol Italia Cty Eresson - hiết quang kế đo o x, đ cồn Trung Quốc SP à N i - chưng cất cồn SMI O Trung Quốc SP à N i - ệ thống chưng cất 20 lit; 100 lít Việt Nam SP à N i/ Cty Eresson - Máy nghiền uhley- Universal ức Cty Eresson - Máy đo nồng đ ôxy hoà tan ORION 5 Star Mỹ Cty Eresson - Tủ ổn nhiệt FO - 225E Italy SP à N i/ Cty Eresson 3PL P ụ lục 2: ôi trƣờn 2.1. M i trườ g giữ giố g a. Mô trườn Hansen [6] (ký ệu l : M1) Thành phần: Nước cất 1000,0 ml Cao men 1,0 g Pepton 10,0 g ường glucose 50,0 g MgSO4.7H2O 3,0 g Aga 20 g KH2PO4 3,0 g pH 6 - 7 b. Mô trườn dịc c ết malt - aga [6] (ký ệu l : M2) Thành phần: Dịch chiết malt (7 0Bx) 1000,0 ml NaCl 1,0 g KH2PO4 1,0 g Cao men 1,0 g MgSO4.7H2O 1,0 g Aga 20 g (NH4)2SO4 3,0 g pH 6 - 7 2.2. M i trườ g hâ si h hối ấm me YPD [5] Môi trường nhân sinh khối (ký hiệu là: M3) c thành phần như sau: Nước cất 1000,0 g Dextrose 20,0 g Cao men 10,0 g Aga 15 g Peptone 20,0 g pH 6,5 - 7,2 - C c mô trườn t an trùn ở 1210C /15 - 20 phút. 2.3. M i trườ g hâ giố g ơ b Môi trường nhân giống cơ bản (ký hiệu là: M6) c thành phần như sau: Dịch chiết malt 10 0Bx pH 5,0 g ách làm: oà tan các chất trong 1000 ml dịch chiết malt 100Bx. Thanh trùng ở 1150C trong 15 - 20 phút. 2.4. M i trườ g xá đị h h ă g si h trưở g, phát triể v me ủ á hủ g ấm me - M i trườ g hâ giố g ấp 1 ( ý hiệu : G1) 1- Pepton 10 (g/l) 3- Dịch malt 180Bx 20%Vol 2- Glucose 50 (g/l) 4- Nước (đủ tới) 1000 ml 3 – ường tổng số 80 (g/l) 5-p (chỉnh bằng citric) 4,5 - M i trườ g hâ giố g ơ b ( ý hiệu : G2) 1- Dịch malt 180Bx 50% V 3- ường tổng số 100 (g/l) 2- iá đ xay 100g/l 4-p (chỉnh bằng citric) 4,5 3 - Nước (đủ tới) 1000 ml 3PL P ụ lục 3: P ân lập nấm men ánh men ắc à Bánh men Làng Vân ánh men Mẫu Sơn ình 1: ình ảnh bánh men truyền thống của Việt Nam 4PL a. ánh giá hình thái khu n lạc b. MS18 d. MS42 e. MS18 g. LV22 c. BH30 ình 2: ình thái khu n lạc m t số mẫu nấm men điển hình 5PL a. Men ắc à b. Men Mẫu Sơn Men Làng Vân ình 3: Mẫu nấm men phân lập từ các nguồn khác nhau 6PL P ụ lục 4: N uy n liệu v tuyển c ọn nấm men a. Malt Buloke (Úc) b. Malt Sebastian (Pháp) c. Ngô DK 9955 ình 4: Mẫu malt, ngô nguyên liệu ình 5: Lên men các d ng nấm men phân lập trên môi trường malt, ngô 7PL a. Lên men của các d ng nấm men Mấu Sơn (MS) b. Lên men các d ng nấm men ắc à ( ) và LàngVân (LV) ình 6: ánh giá lên men của các d ng nấm men truyền thống 8PL P ụ lục 5: Đặc điểm l n men v đán iá c m qu n rƣợu từ các dòn nấm men ẫu Sơn ảng 1: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu malt các d ng nấm men Mẫu Sơn STT Mẫu mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 MS1 18 170,55 2,78 85,73 9,0 71,5 83,35 4,0 0,8 4,0 1,2 3,0 2,0 14,0 2 MS2 18 170,55 3,52 85,35 8,5 67,5 79,07 3,0 0,8 3,0 1,2 2,0 2,0 10,0 3 MS3 18 170,55 4,45 84,88 8,8 69,9 82,32 4,0 0,8 4,0 1,2 4,0 2,0 16,0 4 MS4 18 170,55 3,18 85,53 9,0 71,5 83,55 4,0 0,8 4,0 1,2 4,0 2,0 16,0 5 MS5 18 170,55 4,25 84,98 9,0 71,5 84,09 3,0 0,8 3,0 1,2 3,0 2,0 12,0 6 MS6 18 170,55 5,16 84,51 8,8 69,9 82,67 3,0 0,8 3,0 1,2 3,0 2,0 12,0 7 MS7 18 170,55 5,22 84,48 8,5 67,5 79,89 2,0 0,8 3,0 1,2 3,0 2,0 11,2 8 MS8 18 170,55 4,08 85,07 8,5 67,5 79,34 3,0 0,8 3,0 1,2 3,0 2,0 12,0 9 MS9 18 170,55 5,13 84,53 8,8 69,9 82,66 3,0 0,8 3,0 1,2 3,0 2,0 12,0 10 MS10 18 170,55 2,84 85,70 9,0 71,5 83,38 4,0 0,8 4,0 1,2 4,0 2,0 16,0 11 MS11 18 170,55 3,16 85,54 9,0 71,5 83,54 3,0 0,8 3,0 1,2 3,0 2,0 12,0 12 MS12 18 170,55 3,24 85,50 9,0 71,5 83,58 3,0 0,8 3,0 1,2 3,0 2,0 12,0 13 MS13 18 170,55 1,95 86,15 9,2 73,0 84,79 3,0 0,8 3,0 1,2 3,0 2,0 12,0 14 MS14 18 170,55 3,26 85,49 8,4 66,7 78,02 3,0 0,8 2,0 1,2 3,0 2,0 10,8 15 MS15 18 170,55 2,08 86,09 9,2 73,0 84,85 4,0 0,8 3,0 1,2 4,0 2,0 14,8 16 MS16 18 170,55 3,46 85,38 8,2 65,1 76,25 3,0 0,8 2,0 1,2 3,0 2,0 10,8 17 MS17 18 170,55 5,03 84,58 8,5 67,5 79,79 3,0 0,8 2,0 1,2 3,0 2,0 10,8 18 MS18 18 170,55 3,24 85,50 9,2 73,0 86,44 4,5 0,8 4,3 1,2 3,7 2,0 16,3 9PL 19 MS19 18 170,55 3,25 85,49 8,8 69,9 81,73 3,0 0,8 3,0 1,2 3,0 2,0 12,0 20 MS20 18 170,55 3,89 85,16 9,0 71,5 83,91 3,0 0,8 3,0 1,2 3,0 2,0 12,0 21 MS21 18 170,55 5,23 84,48 8,5 67,5 79,89 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 MS22 18 170,55 4,16 85,03 9,0 71,5 84,05 4,0 0,8 3,0 1,2 4,0 2,0 14,8 23 MS23 18 170,55 4,23 84,99 8,8 69,9 82,21 3,0 0,8 3,0 1,2 4,0 2,0 14,0 24 MS24 18 170,55 3,16 85,54 9,0 71,5 83,54 3,0 0,8 3,0 1,2 3,0 2,0 12,0 25 MS25 18 170,55 5,03 84,58 8,5 67,5 79,79 4,0 0,8 3,0 1,2 4,0 2,0 14,8 26 MS26 18 170,55 5,24 84,47 8,5 67,5 79,89 4,0 0,8 3,0 1,2 4,0 2,0 14,8 27 MS27 18 170,55 2,16 86,05 9,0 71,5 83,05 3,0 0,8 3,0 1,2 3,0 2,0 12,0 28 MS28 18 170,55 6,28 83,94 8,4 66,7 79,45 3,0 0,8 3,0 1,2 3,0 2,0 12,0 29 MS29 18 170,55 5,92 84,13 8,5 67,5 80,22 3,0 0,8 2,0 1,2 3,0 2,0 10,8 30 MS30 18 170,55 5,41 84,39 8,5 67,5 79,98 4,0 0,8 3,0 1,2 4,0 2,0 14,8 31 MS31 18 170,55 5,23 84,48 8,5 67,5 79,89 3,0 0,8 3,0 1,2 2,0 2,0 10,0 32 MS32 18 170,55 3,55 85,34 8,8 69,9 81,88 4,0 0,8 3,0 1,2 3,0 2,0 12,8 33 MS33 18 170,55 4,26 84,97 8,8 69,9 82,23 3,0 0,8 2,0 1,2 2,0 2,0 8,8 34 MS34 18 170,55 2,81 85,72 9,0 71,5 83,37 3,0 0,8 2,0 1,2 3,0 2,0 10,8 35 MS35 18 170,55 4,55 84,83 8,0 63,5 74,88 3,0 0,8 3,0 1,2 3,0 2,0 12,0 36 MS36 18 170,55 3,68 85,27 8,5 67,5 79,15 3,0 0,8 3,0 1,2 3,0 2,0 12,0 37 MS37 18 170,55 5,62 84,28 8,5 67,5 80,08 2,0 0,8 3,0 1,2 3,0 2,0 11,2 38 MS38 18 170,55 2,84 85,70 9,0 71,5 83,38 2,0 0,8 3,0 1,2 3,0 2,0 11,2 39 MS39 18 170,55 3,21 85,51 9,0 71,5 83,57 3,0 0,8 3,0 1,2 3,0 2,0 12,0 40 MS40 18 170,55 3,01 85,61 9,5 75,4 88,11 4,5 0,8 4,1 1,2 3,8 2,0 16,1 41 MS41 18 170,55 5,28 84,45 8,8 69,9 82,73 3,0 0,8 3,0 1,2 3,0 2,0 12,0 42 MS42 18 170,55 2,05 86,10 9,7 77,0 89,45 4,5 0,8 4,3 1,2 3,9 2,0 16,6 43 MS43 18 170,55 5,62 84,28 8,5 67,5 80,08 3,0 0,8 3,0 1,2 3,0 2,0 12,0 10PL ảng 2: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu ngô các d ng nấm men Mẫu Sơn STT Dòng mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 MS1 18 169,3 2,92 85,02 9,0 71,5 84,05 3,0 0,8 4,0 1,2 3,0 2,0 13,2 2 MS2 18 169,3 3,46 84,74 8,3 65,9 77,77 3,0 0,8 3,0 1,2 3,0 2,0 12,0 3 MS3 18 169,3 4,49 84,22 8,6 68,3 81,08 4,0 0,8 4,0 1,2 4,0 2,0 16,0 4 MS4 18 169,3 3,62 84,66 9,0 71,5 84,41 3,0 0,8 3,0 1,2 4,0 2,0 14,0 5 MS5 18 169,3 4,29 84,32 8,8 69,9 82,87 3,0 0,8 3,0 1,2 3,0 2,0 12,0 6 MS6 18 169,3 5,22 83,84 8,6 68,3 81,44 3,0 0,8 2,0 1,2 4,0 2,0 12,8 7 MS7 18 169,3 5,26 83,82 8,3 65,9 78,62 3,0 0,8 2,0 1,2 3,0 2,0 10,8 8 MS8 18 169,3 4,12 84,41 8,3 65,9 78,08 3,0 0,8 3,0 1,2 3,0 2,0 12,0 9 MS9 18 169,3 5,17 83,87 8,6 68,3 81,42 3,0 0,8 3,0 1,2 3,0 2,0 12,0 10 MS10 18 169,3 2,98 84,99 9,0 71,5 84,08 4,0 0,8 4,0 1,2 4,0 2,0 16,0 11 MS11 18 169,3 3,2 84,88 8,8 69,9 82,32 3,0 0,8 3,0 1,2 3,0 2,0 12,0 12 MS12 18 169,3 3,28 84,84 8,8 69,9 82,36 3,0 0,8 3,0 1,2 3,0 2,0 12,0 13 MS13 18 169,3 1,99 85,50 9,0 71,5 83,58 3,0 0,8 3,0 1,2 3,0 2,0 12,0 14 MS14 18 169,3 3,3 84,83 8,2 65,1 76,75 3,0 0,8 3,0 1,2 3,0 2,0 12,0 15 MS15 18 169,3 2,12 85,43 9,0 71,5 83,65 4,0 0,8 3,0 1,2 4,0 2,0 14,8 16 MS16 18 169,3 3,5 84,72 8,0 63,5 74,97 3,0 0,8 2,0 1,2 3,0 2,0 10,8 17 MS17 18 169,3 5,07 83,92 8,3 65,9 78,53 3,0 0,8 2,0 1,2 3,0 2,0 10,8 18 MS18 18 169,3 3,28 84,84 9,2 73,0 86,10 4,5 0,8 4,1 1,2 3,2 2,0 14,9 19 MS19 18 169,3 3,29 84,83 8,6 68,3 80,49 3,0 0,8 3,0 1,2 3,0 2,0 12,0 11PL 20 MS20 18 169,3 3,93 84,50 8,8 69,9 82,68 3,0 0,8 3,0 1,2 3,0 2,0 12,0 21 MS21 18 169,3 5,27 83,82 8,3 65,9 78,62 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 MS22 18 169,3 4,2 84,37 8,8 69,9 82,82 3,0 0,8 3,0 1,2 3,0 2,0 12,0 23 MS23 18 169,3 4,27 84,33 8,8 69,9 82,86 3,0 0,8 3,0 1,2 4,0 2,0 14,0 24 MS24 18 169,3 3,2 84,88 8,8 69,9 82,32 3,0 0,8 3,0 1,2 3,0 2,0 12,0 25 MS25 18 169,3 5,07 83,92 8,3 65,9 78,53 4,0 0,8 3,0 1,2 4,0 2,0 14,8 26 MS26 18 169,3 5,38 83,76 8,3 65,9 78,68 4,0 0,8 2,0 1,2 3,0 2,0 11,6 27 MS27 18 169,3 2,2 85,39 8,8 69,9 81,83 3,0 0,8 3,0 1,2 3,0 2,0 12,0 28 MS28 18 169,3 6,32 83,28 8,2 65,1 78,18 3,0 0,8 3,0 1,2 3,0 2,0 12,0 29 MS29 18 169,3 5,96 83,47 8,3 65,9 78,96 3,0 0,8 2,0 1,2 3,0 2,0 10,8 30 MS30 18 169,3 5,45 83,73 8,3 65,9 78,71 4,0 0,8 3,0 1,2 3,0 2,0 12,8 31 MS31 18 169,3 5,27 83,82 8,3 65,9 78,62 3,0 0,8 3,0 1,2 2,0 2,0 10,0 32 MS32 18 169,3 3,59 84,68 8,6 68,3 80,64 3,0 0,8 3,0 1,2 3,0 2,0 12,0 33 MS33 18 169,3 4,3 84,32 8,6 68,3 80,99 3,0 0,8 2,0 1,2 2,0 2,0 8,8 34 MS34 18 169,3 2,85 85,06 8,8 69,9 82,15 3,0 0,8 2,0 1,2 3,0 2,0 10,8 35 MS35 18 169,3 4,59 84,17 7,8 61,9 73,58 3,0 0,8 3,0 1,2 3,0 2,0 12,0 36 MS36 18 169,3 3,72 84,61 8,3 65,9 77,89 3,0 0,8 3,0 1,2 3,0 2,0 12,0 37 MS37 18 169,3 5,66 83,62 8,3 65,9 78,81 2,0 0,8 2,0 1,2 3,0 2,0 10,0 38 MS38 18 169,3 2,88 85,04 9,0 71,5 84,03 2,0 0,8 3,0 1,2 3,0 2,0 11,2 39 MS39 18 169,3 3,25 84,85 8,8 69,9 82,35 3,0 0,8 3,0 1,2 3,0 2,0 12,0 40 MS40 18 169,3 3,05 84,95 9,4 74,6 87,85 4,5 0,8 4,1 1,2 3,2 2,0 14,9 41 MS41 18 169,3 5,32 83,79 8,6 68,3 81,49 3,0 0,8 3,0 1,2 3,0 2,0 12,0 42 MS42 18 169,3 2,16 85,41 9,5 75,4 88,32 4,8 0,8 4,2 1,2 3,5 2,0 15,9 43 MS43 18 169,3 5,66 83,62 8,5 67,5 80,71 3,0 0,8 2,0 1,2 3,0 2,0 10,8 12PL P ụ lục 6: Đặc điểm l n men v đán iá c m qu n rƣợu từ các dòn nấm men ắc H ảng 3: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu malt các d ng nấm men ắc à STT Dòng mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 BH1 18 170,55 3,24 85,50 9,0 71,5 83,58 4,3 0,8 3,1 1,2 3,9 2,0 15,0 2 BH2 18 170,55 2,95 85,64 9,0 71,5 83,44 4,6 0,8 3,9 1,2 3,7 2,0 15,8 3 BH3 18 170,55 3,26 85,49 9,0 71,5 83,59 4,0 0,8 4,0 1,2 3,0 2,0 14,0 4 BH4 18 170,55 2,08 86,09 9,0 71,5 83,01 4,0 0,8 4,0 1,2 3,0 2,0 14,0 5 BH5 18 170,55 3,46 85,38 9,0 71,5 83,69 3,0 0,8 3,0 1,2 3,0 2,0 12,0 6 BH6 18 170,55 5,03 84,58 8,8 69,9 82,61 3,0 0,8 3,0 1,2 3,0 2,0 12,0 7 BH7 18 170,55 3,24 85,50 9,0 71,5 83,58 2,0 0,8 3,0 1,2 3,0 2,0 11,2 8 BH8 18 170,55 3,25 85,49 9,0 71,5 83,59 3,0 0,8 3,0 1,2 3,0 2,0 12,0 9 BH9 18 170,55 3,89 85,16 8,8 69,9 82,04 3,0 0,8 4,0 1,2 3,0 2,0 13,2 10 BH10 18 170,55 5,23 84,48 8,8 69,9 82,71 3,0 0,8 4,0 1,2 4,0 2,0 15,2 11 BH11 18 170,55 4,16 85,03 8,5 67,5 79,38 3,0 0,8 3,0 1,2 3,0 2,0 12,0 12 BH12 18 170,55 3,24 85,50 8,8 69,9 81,73 3,0 0,8 3,0 1,2 3,0 2,0 12,0 13 BH13 18 170,55 1,95 86,15 9,2 73,0 84,79 3,0 0,8 3,0 1,2 3,0 2,0 12,0 14 BH14 18 170,55 3,26 85,49 8,4 66,7 78,02 3,0 0,8 2,0 1,2 3,0 2,0 10,8 15 BH15 18 170,55 2,08 86,09 9,2 73,0 84,85 4,0 0,8 3,0 1,2 4,0 2,0 14,8 16 BH16 18 170,55 3,46 85,38 8,2 65,1 76,25 3,0 0,8 2,0 1,2 3,0 2,0 10,8 17 BH17 18 170,55 5,03 84,58 8,5 67,5 79,79 3,0 0,8 2,0 1,2 3,0 2,0 10,8 18 BH18 18 170,55 3,24 85,50 9,0 71,5 83,58 3,0 0,8 4,0 1,2 4,0 2,0 15,2 19 BH19 18 170,55 3,25 85,49 9,5 75,4 88,23 4,5 0,8 3,9 1,2 3,9 2,0 16,1 13PL 20 BH20 18 170,55 2,16 86,05 8,8 69,9 81,20 4,1 0,8 3,1 1,2 4,2 2,0 15,4 21 BH21 18 170,55 6,28 83,94 8,5 67,5 80,40 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 BH22 18 170,55 5,92 84,13 8,5 67,5 80,22 4,0 0,8 3,0 1,2 4,0 2,0 14,8 23 BH23 18 170,55 5,41 84,39 9,0 71,5 84,68 3,0 0,8 3,0 1,2 4,0 2,0 14,0 24 BH24 18 170,55 5,23 84,48 9,2 73,0 86,47 3,5 0,8 4,1 1,2 3,9 2,0 15,5 25 BH25 18 170,55 3,55 85,34 8,5 67,5 79,09 4,0 0,8 3,0 1,2 4,0 2,0 14,8 26 BH26 18 170,55 4,26 84,97 9,0 71,5 84,10 4,0 0,8 3,0 1,2 4,0 2,0 14,8 27 BH27 18 170,55 2,81 85,72 9,0 71,5 83,37 3,0 0,8 3,0 1,2 3,0 2,0 12,0 28 BH28 18 170,55 4,55 84,83 8,4 66,7 78,63 3,0 0,8 3,0 1,2 3,0 2,0 12,0 29 BH29 18 170,55 3,68 85,27 8,5 67,5 79,15 3,0 0,8 2,0 1,2 3,0 2,0 10,8 30 BH30 18 170,55 5,62 84,28 9,0 71,5 84,79 4,6 0,8 4,1 1,2 3,8 2,0 16,2 31 BH31 18 170,55 2,84 85,70 8,5 67,5 78,75 3,0 0,8 3,0 1,2 2,0 2,0 10,0 32 BH32 18 170,55 3,21 85,51 8,8 69,9 81,71 4,0 0,8 3,0 1,2 3,0 2,0 12,8 33 BH33 18 170,55 3,01 85,61 9,5 75,4 88,11 4,2 0,8 3,4 1,2 4,2 2,0 15,8 34 BH34 18 170,55 5,28 84,45 9,0 71,5 84,62 4,1 0,8 3,2 1,2 4,1 2,0 15,3 35 BH35 18 170,55 4,55 84,83 9,0 71,5 84,24 3,0 0,8 3,0 1,2 3,0 2,0 12,0 36 BH36 18 170,55 3,68 85,27 9,0 71,5 83,80 3,0 0,8 3,0 1,2 3,0 2,0 12,0 37 BH37 18 170,55 5,62 84,28 8,5 67,5 80,08 2,0 0,8 3,0 1,2 3,0 2,0 11,2 38 BH38 18 170,55 2,84 85,70 8,5 67,5 78,75 2,0 0,8 3,0 1,2 3,0 2,0 11,2 39 BH39 18 170,55 3,21 85,51 8,8 69,9 81,71 3,0 0,8 3,0 1,2 3,0 2,0 12,0 40 BH40 18 170,55 3,01 85,61 8,8 69,9 81,61 3,0 0,8 4,0 1,2 4,0 2,0 15,2 41 BH41 18 170,55 5,28 84,45 8,5 67,5 79,91 3,9 0,8 3,1 1,2 4,3 2,0 15,4 42 BH42 18 170,55 2,95 85,64 9,2 73,0 85,29 4,2 0,8 3,8 1,2 3,6 2,0 15,2 14PL ảng 4: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu ngô các d ng nấm men ắc à STT Dòng mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 BH1 18 169,3 3,64 84,65 9,0 71,5 84,42 4,2 0,8 4,2 1,2 3,2 2,0 14,8 2 BH2 18 169,3 2,15 85,41 9,0 71,5 83,66 4,2 0,8 4,1 1,2 3,1 2,0 14,5 3 BH3 18 169,3 3,66 84,64 8,0 63,5 75,05 4,0 0,8 4,0 1,2 3,0 2,0 14,0 4 BH4 18 169,3 2,48 85,25 7,8 61,9 72,65 4,0 0,8 3,0 1,2 3,0 2,0 12,8 5 BH5 18 169,3 3,86 84,54 7,8 61,9 73,26 4,0 0,8 3,0 1,2 3,0 2,0 12,8 6 BH6 18 169,3 5,43 83,74 7,6 60,3 72,06 4,0 0,8 3,0 1,2 3,0 2,0 12,8 7 BH7 18 169,3 3,84 84,55 8,0 63,5 75,13 4,0 0,8 3,0 1,2 3,0 2,0 12,8 8 BH8 18 169,3 3,55 84,70 7,8 61,9 73,12 4,0 0,8 3,0 1,2 3,0 2,0 12,8 9 BH9 18 169,3 4,29 84,32 7,6 60,3 71,57 4,0 0,8 3,0 1,2 3,0 2,0 12,8 10 BH10 18 169,3 5,63 83,64 7,6 60,3 72,15 4,0 0,8 4,0 1,2 3,0 2,0 14,0 11 BH11 18 169,3 4,56 84,18 7,3 58,0 68,85 4,0 0,8 3,0 1,2 3,0 2,0 12,8 12 BH12 18 169,3 3,64 84,65 7,6 60,3 71,28 4,0 0,8 3,0 1,2 3,0 2,0 12,8 13 BH13 18 169,3 2,35 85,31 8,0 63,5 74,46 4,0 0,8 3,0 1,2 3,0 2,0 12,8 14 BH14 18 169,3 3,66 84,64 7,2 57,2 67,54 4,0 0,8 2,0 1,2 3,0 2,0 11,6 15 BH15 18 169,3 2,48 85,25 9,2 73,0 85,69 4,0 0,8 3,0 1,2 4,0 2,0 14,8 16 BH16 18 169,3 3,86 84,54 7,0 55,6 65,74 4,0 0,8 2,0 1,2 3,0 2,0 11,6 17 BH17 18 169,3 5,43 83,74 7,3 58,0 69,22 4,0 0,8 2,0 1,2 3,0 2,0 11,6 18 BH18 18 169,3 3,64 84,65 7,8 61,9 73,16 4,0 0,8 4,0 1,2 4,0 2,0 16,0 19 BH19 18 169,3 3,05 84,65 9,00 71,5 87,42 4,1 0,8 4,0 1,2 3,5 2,0 15,1 20 BH20 18 169,3 2,56 85,20 8,50 67,5 79,21 3,6 0,8 4,3 1,2 3,1 2,0 14,2 15PL 21 BH21 18 169,3 6,68 83,10 7,3 58,0 69,75 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 BH22 18 169,3 6,52 83,18 7,3 58,0 69,68 4,0 0,8 3,0 1,2 4,0 2,0 14,8 23 BH23 18 169,3 5,81 83,54 7,8 61,9 74,13 3,0 0,8 3,0 1,2 4,0 2,0 14,0 24 BH24 18 169,3 5,63 83,64 8,50 67,5 80,70 3,2 0,8 4,1 1,2 3,6 2,0 14,7 25 BH25 18 169,3 3,95 84,49 7,5 59,6 70,48 4,0 0,8 3,0 1,2 4,0 2,0 14,8 26 BH26 18 169,3 4,56 84,18 7,8 61,9 73,57 4,0 0,8 3,0 1,2 4,0 2,0 14,8 27 BH27 18 169,3 3,21 84,87 7,8 61,9 72,97 3,0 0,8 3,0 1,2 3,0 2,0 12,0 28 BH28 18 169,3 4,95 83,98 7,2 57,2 68,07 3,0 0,8 3,0 1,2 3,0 2,0 12,0 29 BH29 18 169,3 4,08 84,43 7,3 58,0 68,65 3,0 0,8 2,0 1,2 3,0 2,0 10,8 30 BH30 18 169,3 4,02 84,46 9,00 71,5 84,61 4,5 0,8 4,1 1,2 3,5 2,0 15,5 31 BH31 18 169,3 3,24 84,86 7,3 58,0 68,31 3,0 0,8 3,0 1,2 3,0 2,0 12,0 32 BH32 18 169,3 3,61 84,67 7,6 60,3 71,27 4,0 0,8 3,0 1,2 3,0 2,0 12,8 33 BH33 18 169,3 3,41 84,77 9,30 73,8 87,11 3,9 0,8 4,2 1,2 3,2 2,0 14,6 34 BH34 18 169,3 3,28 84,84 9,00 71,5 84,23 4,2 0,8 4,1 1,2 3,2 2,0 14,7 35 BH35 18 169,3 4,95 83,98 7,8 61,9 73,74 3,0 0,8 3,0 1,2 3,0 2,0 12,0 36 BH36 18 169,3 4,08 84,43 7,8 61,9 73,36 3,0 0,8 3,0 1,2 3,0 2,0 12,0 37 BH37 18 169,3 6,02 83,44 7,3 58,0 69,47 2,0 0,8 3,0 1,2 3,0 2,0 11,2 38 BH38 18 169,3 3,24 84,86 7,3 58,0 68,31 2,0 0,8 3,0 1,2 3,0 2,0 11,2 39 BH39 18 169,3 3,85 84,54 7,6 60,3 71,38 3,0 0,8 3,0 1,2 3,0 2,0 12,0 40 BH40 18 169,3 3,41 84,77 7,6 60,3 71,19 4,0 0,8 3,0 1,2 4,0 2,0 14,8 41 BH41 18 169,3 5,68 83,61 8,50 67,5 80,72 3,9 0,8 4,1 1,2 3,5 2,0 15,0 42 BH42 18 169,3 3,05 84,95 9,00 71,5 84,12 4,3 0,8 3,8 1,2 3,2 2,0 14,4 16PL P ụ lục 7: Đặc điểm l n men v đán iá c m qu n rƣợu từ các dòn nấm men Làng Vân ảng 5: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu malt các d ng nấm men Làng Vân STT Dòng mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 LV1 18 170,55 4,24 84,98 7,8 61,9 72,87 3,5 0,8 4,0 1,2 3,0 2,0 13,6 2 LV2 18 170,55 2,68 85,78 8,0 63,5 74,05 3,5 0,8 3,5 1,2 3,0 2,0 13,0 3 LV3 18 170,55 3,26 85,49 8,0 63,5 74,31 3,2 0,8 3,0 1,2 4,0 2,0 14,2 4 LV4 18 170,55 2,08 86,09 8,5 67,5 78,40 3,6 0,8 3,9 1,2 3,7 2,0 15,0 5 LV5 18 170,55 3,46 85,38 7,8 61,9 72,53 3,0 0,8 3,0 1,2 3,0 2,0 12,0 6 LV6 18 170,55 5,03 84,58 7,5 59,6 70,41 3,5 0,8 3,0 1,2 3,0 2,0 12,4 7 LV7 18 170,55 4,24 84,98 8,0 63,5 74,74 3,5 0,8 3,2 1,2 3,0 2,0 12,6 8 LV8 18 170,55 3,25 85,49 8,0 63,5 74,30 3,5 0,8 3,0 1,2 3,0 2,0 12,4 9 LV9 18 170,55 3,89 85,16 7,8 61,9 72,72 3,0 0,8 3,0 1,2 3,0 2,0 12,0 10 LV10 18 170,55 5,23 84,48 7,5 59,6 70,49 3,5 0,8 3,0 1,2 4,0 2,0 14,4 11 LV11 18 170,55 4,16 85,03 7,8 61,9 72,84 3,5 0,8 3,0 1,2 3,0 2,0 12,4 12 LV12 18 170,55 3,24 85,50 8,2 65,1 76,15 3,0 0,8 3,0 1,2 3,0 2,0 12,0 13 LV13 18 170,55 1,95 86,15 8,2 65,1 75,57 3,2 0,8 3,0 1,2 3,0 2,0 12,2 14 LV14 18 170,55 3,26 85,49 7,4 58,8 68,73 3,2 0,8 3,0 1,2 3,0 2,0 12,2 15 LV15 18 170,55 2,08 86,09 8,2 65,1 75,63 3,0 0,8 3,0 1,2 4,0 2,0 14,0 17PL 16 LV16 18 170,55 4,46 84,87 7,2 57,2 67,36 3,5 0,8 3,0 1,2 3,0 2,0 12,4 17 LV17 18 170,55 5,03 84,58 7,5 59,6 70,41 3,5 0,8 3,0 1,2 3,0 2,0 12,4 18 LV18 18 170,55 3,25 85,49 8,2 65,1 76,16 3,5 0,8 3,0 1,2 4,0 2,0 14,4 19 LV19 18 170,55 3,25 85,49 8,5 67,5 78,94 3,5 0,8 4,0 1,2 3,5 2,0 14,6 20 LV20 18 170,55 2,15 86,05 7,8 61,9 71,97 3,2 0,8 3,0 1,2 3,0 2,0 12,2 21 LV21 18 170,55 6,28 83,94 7,5 59,6 70,94 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 LV22 18 170,55 3,26 85,49 9,2 73,0 85,45 4,6 0,8 4,2 1,2 3,8 2,0 16,3 18PL ảng 6: Tổng hợp lên men và đánh giá cảm quan rượu từ nguyên liệu ngô các d ng nấm men Làng Vân STT Dòng mấm men o Bx ường khử (quy về maltose) g/l ường sót (quy về maltose) g/l Khối lượng rượu theo lý thuyết (g) Phần trăm cồn thực tế (% v/v) Khối lượng rượu theo thực tế (g) iệu suất lên men rượu (%) ánh giá cảm quan (T VN 3215-79) trong và màu sắc ệ số (0,8) Mùi ệ số (1,2) Vị ệ số (2,0) Tổng điểm 1 LV1 18 169,3 4,54 84,19 7,8 61,9 73,56 4,0 0,8 4,0 1,2 3,0 2,0 14,0 2 LV2 18 169,3 3,28 84,84 7,0 55,6 65,51 3,0 0,8 4,0 1,2 3,0 2,0 13,2 3 LV3 18 169,3 3,46 84,74 7,8 61,9 73,08 4,0 0,8 4,0 1,2 3,0 2,0 14,0 4 LV4 18 169,3 2,58 85,19 8,5 67,5 79,22 4,5 0,8 4,1 1,2 3,2 2,0 14,9 5 LV5 18 169,3 3,96 84,49 7,6 60,3 71,42 3,0 0,8 3,0 1,2 3,0 2,0 12,0 6 LV6 18 169,3 5,23 83,84 7,3 58,0 69,13 3,0 0,8 3,0 1,2 3,0 2,0 12,0 7 LV7 18 169,3 4,74 84,09 8,0 63,5 75,54 2,0 0,8 3,0 1,2 3,0 2,0 11,2 8 LV8 18 169,3 3,75 84,60 7,8 61,9 73,21 3,0 0,8 3,0 1,2 3,0 2,0 12,0 9 LV9 18 169,3 4,59 84,17 7,6 60,3 71,70 3,0 0,8 3,0 1,2 3,0 2,0 12,0 10 LV10 18 169,3 5,73 83,58 7,3 58,0 69,35 4,0 0,8 4,0 1,2 3,0 2,0 14,0 11 LV11 18 169,3 4,66 84,13 7,6 60,3 71,73 3,0 0,8 3,0 1,2 3,0 2,0 12,0 12 LV12 18 169,3 3,54 84,70 8,0 63,5 74,99 3,0 0,8 3,0 1,2 3,0 2,0 12,0 13 LV13 18 169,3 2,45 85,26 8,0 63,5 74,50 3,0 0,8 3,0 1,2 3,0 2,0 12,0 14 LV14 18 169,3 3,76 84,59 7,2 57,2 67,58 3,0 0,8 2,0 1,2 3,0 2,0 10,8 15 LV15 18 169,3 2,58 85,19 8,0 63,5 74,56 4,0 0,8 3,0 1,2 4,0 2,0 14,8 16 LV16 18 169,3 4,96 83,98 8,0 63,5 75,64 3,0 0,8 3,0 1,2 3,0 2,0 12,0 19PL 17 LV17 18 169,3 5,53 83,69 7,5 59,6 71,16 3,0 0,8 3,0 1,2 3,0 2,0 12,0 18 LV18 18 169,3 3,55 84,70 7,8 61,9 73,12 4,0 0,8 4,0 1,2 3,0 2,0 14,0 19 LV19 18 169,3 3,75 84,60 8,3 65,9 77,90 5,0 0,8 5,0 1,2 4,0 2,0 18,0 20 LV20 18 169,3 2,65 85,16 8,5 67,5 79,25 3,0 0,8 3,0 1,2 3,0 2,0 12,0 21 LV21 18 169,3 6,78 83,05 8,3 65,9 79,35 4,0 0,8 3,0 1,2 4,0 2,0 14,8 22 LV22 18 169,3 3,26 84,85 9,0 71,5 84,22 4,5 0,8 3,9 1,2 3,5 2,0 15,3 20PL P ụ lục 8: Hìn n tế b o nấm men s u tuyển c ọn l n 1 BH1 BH2 BH19 BH20 BH24 BH30 BH33 BH34 BH41 BH42 LV4 LV22 MS18 MS40 MS42 ình 5: ình ảnh tế bào 15 chủng nấm men đ tuyển chọn lần 1 21PL P ụ lục 9: ết qu kiểm tr kit t ử k n n sử dụn đƣờn củ S42 a. Kết quả xác định khả năng sử dụng đường của chủng MS42 với kit ID 32 b. Kết quả xác định khả năng sử dụng đường của chủng MS42 với kit API 20 AUX ình 7. Kết quả kit thử khả năng sử dụng đường của chủng MS42 22PL P ụ lục 10: Các mẫu ỗ sồi sử dụn tron n i n cứu s n xuất rƣợu w isky từ n ô v m lt đ i m c ình 7: ác mẫu g sồi Mỹ 23PL ình 8: ác mẫu g sồi Pháp ình 9: ác mẫu g sồi Australia 24PL P ụ lục 11: ột số ìn n s n xuất rƣợu t i n máy bi rƣợu resson Máy nghiền Nồi đường h a ệ thống làm lạnh Tank nhân giống Tank lên men Thiết bị chưng cất chân không ệ thống điều khiển Kiểm tra chất lượng nấm men Lấy mẫu rượu phân tích ình 6: M t số hình ảnh sản xuất thực nghiệm tại nhà máy rượu bia RISSON P ụ lục 12: Sắc ký đồ các mẫu rƣợu trắn trƣớc k i n âm ỗ sồi 1. Mẫu M 100 (6/05/2016) 2. Mẫu N 00 (6/05/2016) 3. Mẫu 8N:2M (6/05/2016) P ụ lục 13: Sắc ký đồ các mẫu rƣợu n âm ỗ sồi 06 t án (S8.M, S8.N, S8.N8:M2) 1. Mẫu S8.M (5/11/2016) 2. Mẫu S8.N8:M2 (5/11/2016) 3. Mẫu: S8.N (5/11/2016) P ụ lục 14: Sắc ký đồ các mẫu rƣợu n âm ỗ sồi 12 t án 1. Mẫu S8.M (10/05/2017) 2. Mẫu S8.N (6/05/2017) 3. Mẫu S8. N8:M2 (10/05/2017) P ụ lục 15: Sắc ký đồ các mẫu rƣợu n âm ỗ sồi 18 t án 1. Mẫu: S8.M (10/11/2017) 2. Mẫu: S8.N (6/11/2017) 3. Mẫu S8. N8:M2 (10/11/2017) P ụ lục 16: Sắc ký đồ các mẫu rƣợu n âm ỗ sồi 24 t án 1. Mẫu: S8.M (2/05/2018) 2. Mẫu S8.N (2/05/2018) 4. Mẫu: S8.N8:M2 (2/05/2018)