Luận án Đặc điểm sinh học, di truyền và phòng chống sinh học nấm fusarium oxysporum f.sp.cubense (foc) gây bệnh héo vàng chuối ở miền Bắc Việt Nam nhà xuất

GTGGG GCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAAG CAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAG GCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATGT TGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >9 Fusarium oxysporum isolate Foc 7 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 7] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >10 Fusarium oxysporum isolate Foc 21 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 21] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC 163 ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >11 Fusarium oxysporum isolate Foc 5 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 5] MTCACCTTAACGTSGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >12 Fusarium oxysporum isolate Foc 1 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 1] MTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >13 Fusarium oxysporum isolate Foc 16 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 16] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >14 Fusarium oxysporum isolate Foc 23-2 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 23-2] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >15 Fusarium oxysporum isolate Foc 2 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 2] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTGC CATTGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCTA 164 CCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCCA TCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGG GGCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTTCCATTCTCACAACCTCGATGAGTGCGTCGTCACGTGTCAA GCAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAA GGCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATG TTGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >16 Fusarium oxysporum isolate Foc 38 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 38] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATCGTCAATCCCGACCAAGACCTGGCAGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCAATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGTTACCCCGCTCGAGACCAAAATTTTGCAATATGACCGTAATTTTTTTTGGTGGG GCACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTACCATTCTCACAACCTCAATGAGTGCGTCGTCACGTGTCAAG CAGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAG GCCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATGT TGTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >17 Fusarium oxysporum isolate Foc 10 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 10] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCATATCTG CCATCGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCAATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAATTTTGCAATATGACCGTAATTTTTTGGTGGGG CACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTACCATTCTCAGAACCTCAATGAGTGCGTCGTCACGTGTCAAGC AGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAGG CCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATGTT GTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >18 Fusarium oxysporum isolate Foc 4 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 4] MTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCATATCTG CCATCGTCAATCCCGACCAAGACCTGGCGGGGTATTTCTCAAAGTCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCAATCGCGCGTCCTTTGCCC ATCGATTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAATTTTGCAATATGACCGTAATTTTTTGGTGGGG CACTTACCCCGCCACTTGAGCGACGGGAGCGTTTGCCCTCTTACCATTCTCAGAACCTCAATGAGTGCGTCGTCACGTGTCAAGC AGTCACTAACCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAGG CCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATGTT GTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAACATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC >19 Fusarium oxysporum isolate Foc 61 partial tef1 genee for translation elongation factor 1 alpha. exons 1-4 [organism=Fusarium oxysporum][isolate=Foc 61] CTCACCTTAACGTCGTCGTCATCGGCCACGTCGACTCTGGCAAGTCGACCACTGTGAGTACTCTCCTCGACAATGAGCTTATCTG CCATCGTCAATCCCGACCAAGACCTGGCGGGGTACTTCTCAAAGGCAACATACTGACATCGTTTCACAGACCGGTCACTTGATCT ACCAGTGCGGTGGTATCGACAAGCGAACCATCGAGAAGTTCGAGAAGGTTAGTCACTTTCCCTTCGATCGCGCGTCCTTTGCCC ATCGACTTCCCCTACGACTCGAAACGTGCCCGCTACCCCGCTCGAGACCAAAAATTTTGCAATATGACCGTAATTTTTTTGGTGG GGCATTTACCCCGCCACTTGAGCGACGGGGCGCGTTTGCCCTCTTACCATTACAACCTCAATGAGTGCGTCGTCACGTGTCAAGC AGTCACTAATCATTCAACAATAGGAAGCCGCTGAGCTCGGTAAGGGTTCCTTCAAGTACGCCTGGGTTCTTGACAAGCTCAAGG CCGAGCGTGAGCGTGGTATCACCATCGATATTGCTCTCTGGAAGTTCGAGACTCCTCGCTACTATGTCACCGTCATTGGTATGTT GTCGCTCATGCTTCATTCTACTTCTCTTCGTACTAATATATCACTCAGACGCTCCCGGTCACCGTGATTTCATCAAGAAC 165 PHỤ LỤC 4. SO SÁNH TRÌNH TỰ CÁC ĐOẠN GENE Gene rpb1 166 Gene rpb2 167 168 Gene tef1α 169 170 171 172 PHỤ LỤC 5. PHÂN TÍCH THỐNG KÊ THI NGHIỆM NHIỆTĐỘ ĐỐI VỚI CHỦNG Foc-TR4 BALANCED ANOVA FOR VARIATE SNC2N FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 1 VARIATE V003 SNC2N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 5 3211.30 642.260 ****** 0.000 2 * RESIDUAL 24 7.90007 .329170 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 29 3219.20 111.007 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC4N FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 2 VARIATE V004 SNC4N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 5 10289.3 2057.86 ****** 0.000 2 * RESIDUAL 24 15.5011 .645879 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 29 10304.8 355.338 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC6N FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 3 VARIATE V005 SNC6N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 5 19581.4 3916.27 ****** 0.000 2 * RESIDUAL 24 16.6005 .691688 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 29 19598.0 675.792 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC8N FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 4 VARIATE V006 SNC8N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 5 35821.4 7164.27 ****** 0.000 2 * RESIDUAL 24 8.70479 .362700 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 29 35830.1 1235.52 ----------------------------------------------------------------------------- TABLE OF MEANS FOR FACTORIAL EFFECTS FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 5 MEANS FOR EFFECT CT ----------------------------------------------------------------------------- -- CT NOS SNC2N SNC4N SNC6N SNC8N 1 5 9.80000 16.2000 23.6000 34.6000 2 5 20.7000 42.5000 64.1000 90.0000 3 5 27.3000 50.8000 72.2000 90.0000 4 5 27.6000 49.9000 60.9000 85.7000 5 5 26.2000 31.4000 34.4000 36.6000 173 6 5 0.000000 0.000000 0.000000 0.000000 SE(N= 5) 0.256581 0.359410 0.371938 0.269332 5%LSD 24DF 0.748889 1.04902 1.08558 0.786106 ----------------------------------------------------------------------------- -- ANALYSIS OF VARIANCE SUMMARY TABLE FILE NDOFUSA 15/ 5/23 10:23 ------------------------------------------------------------------ :PAGE 6 F-PROBABLIITY VALUES FOR EACH EFFECT IN THE MODEL. SECTION - 1 VARIATE GRAND MEAN STANDARD DEVIATION C OF V |CT | (N= 30) -------------------- SD/MEAN | | NO. BASED ON BASED ON % | | OBS. TOTAL SS RESID SS | | SNC2N 30 18.600 10.536 0.57373 3.1 0.0000 SNC4N 30 31.800 18.850 0.80367 2.5 0.0000 SNC6N 30 42.533 25.996 0.83168 2.0 0.0000 SNC8N 30 56.150 35.150 0.60225 1.1 0.0000 THI NGHIỆM pH ĐỐI VỚI CHỦNG Foc-TR4 BALANCED ANOVA FOR VARIATE SNC2N FILE PHFUSA 15/ 5/23 10:51 ------------------------------------------------------------------ :PAGE 1 VARIATE V003 SNC2N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 8 3603.96 450.495 ****** 0.000 2 * RESIDUAL 36 10.4498 .290273 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 44 3614.41 82.1457 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC4N FILE PHFUSA 15/ 5/23 10:51 ------------------------------------------------------------------ :PAGE 2 VARIATE V004 SNC4N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 8 3697.90 462.237 720.35 0.000 2 * RESIDUAL 36 23.1006 .641682 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 44 3721.00 84.5682 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC6N FILE PHFUSA 15/ 5/23 10:51 ------------------------------------------------------------------ :PAGE 3 VARIATE V005 SNC6N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 8 9679.07 1209.88 ****** 0.000 2 * RESIDUAL 36 20.5834 .571762 174 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 44 9699.65 220.447 ----------------------------------------------------------------------------- BALANCED ANOVA FOR VARIATE SNC8N FILE PHFUSA 15/ 5/23 10:51 ------------------------------------------------------------------ :PAGE 4 VARIATE V006 SNC8N LN SOURCE OF VARIATION DF SUMS OF MEAN F RATIO PROB ER SQUARES SQUARES LN ============================================================================= 1 CT 8 8480.58 1060.07 ****** 0.000 2 * RESIDUAL 36 12.5009 .347246 ----------------------------------------------------------------------------- * TOTAL (CORRECTED) 44 8493.08 193.024 ----------------------------------------------------------------------------- TABLE OF MEANS FOR FACTORIAL EFFECTS FILE PHFUSA 15/ 5/23 10:51 ------------------------------------------------------------------ :PAGE 5 MEANS FOR EFFECT CT ----------------------------------------------------------------------------- -- CT NOS SNC2N SNC4N SNC6N SNC8N 1 5 0.000000 31.8000 41.1000 51.8000 2 5 17.7500 33.0000 44.5000 63.0000 3 5 24.3000 40.8000 51.8000 71.6000 4 5 25.7000 51.7000 73.1000 86.5000 5 5 27.8000 52.0000 74.7000 90.0000 6 5 28.6000 53.6000 77.5000 90.0000 7 5 28.8000 54.7000 77.8600 90.0000 8 5 28.5000 54.9000 76.6000 90.0000 9 5 29.2000 55.0000 77.8600 90.0000 SE(N= 5) 0.240945 0.358241 0.338160 0.263532 5%LSD 36DF 0.691030 1.02743 0.969843 0.755809 ----------------------------------------------------------------------------- -- Hiệu lực đối kháng các loại vi khuẩn One-way ANOVA: 3NSC versus cong thuc Source DF SS MS F P cong thuc 3 313.3 104.4 6.55 0.015 Error 8 127.6 16.0 Total 11 441.0 S = 3.994 R-Sq = 71.06% R-Sq(adj) = 60.20% Individual 95% CIs For Mean Based on Pooled StDev Level N Mean StDev -------+---------+---------+---------+-- BV 3 19.048 4.762 (--------*--------) ĐTK1 3 14.286 4.762 (--------*--------) YB11 3 7.018 3.039 (--------*--------) YB4 3 7.018 3.039 (--------*--------) -------+---------+---------+---------+-- 175 6.0 12.0 18.0 24.0 Pooled StDev = 3.994 Grouping Information Using Fisher Method cong thuc N Mean Grouping BV 3 19.048 A ĐTK1 3 14.286 A B YB4 3 7.018 B YB11 3 7.018 B Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of cong thuc Simultaneous confidence level = 82.43% cong thuc = BV subtracted from: cong thuc Lower Center Upper +---------+---------+---------+--------- ĐTK1 -12.283 -4.762 2.759 (------*-------) YB11 -19.551 -12.030 -4.509 (-------*------) YB4 -19.551 -12.030 -4.509 (-------*------) +---------+---------+---------+--------- -20 -10 0 10 cong thuc = ĐTK1 subtracted from: cong thuc Lower Center Upper +---------+---------+---------+--------- YB11 -14.789 -7.268 0.253 (-------*------) YB4 -14.789 -7.268 0.253 (-------*------) +---------+---------+---------+--------- -20 -10 0 10 cong thuc = YB11 subtracted from: cong thuc Lower Center Upper +---------+---------+---------+--------- YB4 -7.521 0.000 7.521 (-------*-------) +---------+---------+---------+--------- -20 -10 0 10 One-way ANOVA: 5NSC versus cong thuc Source DF SS MS F P cong thuc 3 117.4 39.1 1.23 0.362 Error 8 255.6 31.9 Total 11 373.0 S = 5.652 R-Sq = 31.48% R-Sq(adj) = 5.79% Individual 95% CIs For Mean Based on Pooled StDev 176 Level N Mean StDev +---------+---------+---------+--------- BV 3 45.192 7.632 (-----------*------------) ĐTK1 3 42.308 2.885 (------------*-----------) YB11 3 38.384 1.750 (------------*------------) YB4 3 37.374 7.626 (-----------*------------) +---------+---------+---------+--------- 30.0 36.0 42.0 48.0 Pooled StDev = 5.652 Grouping Information Using Fisher Method cong thuc N Mean Grouping BV 3 45.192 A ĐTK1 3 42.308 A YB11 3 38.384 A YB4 3 37.374 A Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of cong thuc Simultaneous confidence level = 82.43% cong thuc = BV subtracted from: cong thuc Lower Center Upper --------+---------+---------+---------+- ĐTK1 -13.527 -2.885 7.757 (----------*----------) YB11 -17.451 -6.808 3.834 (---------*----------) YB4 -18.461 -7.819 2.824 (---------*----------) --------+---------+---------+---------+- -10 0 10 20 cong thuc = ĐTK1 subtracted from: cong thuc Lower Center Upper --------+---------+---------+---------+- YB11 -14.566 -3.924 6.718 (----------*----------) YB4 -15.576 -4.934 5.708 (----------*----------) --------+---------+---------+---------+- -10 0 10 20 cong thuc = YB11 subtracted from: cong thuc Lower Center Upper --------+---------+---------+---------+- YB4 -11.652 -1.010 9.632 (----------*----------) --------+---------+---------+---------+- -10 0 10 20 One-way ANOVA: 7NSC versus cong thuc Source DF SS MS F P cong thuc 3 114.5 38.2 2.50 0.133 Error 8 121.9 15.2 Total 11 236.3 177 S = 3.903 R-Sq = 48.43% R-Sq(adj) = 29.10% Individual 95% CIs For Mean Based on Pooled StDev Level N Mean StDev ---+---------+---------+---------+------ BV 3 61.333 6.110 (----------*---------) ĐTK1 3 58.667 3.055 (---------*----------) YB11 3 53.793 3.160 (----------*---------) YB4 3 54.483 2.069 (---------*---------) ---+---------+---------+---------+------ 50.0 55.0 60.0 65.0 Pooled StDev = 3.903 Grouping Information Using Fisher Method cong thuc N Mean Grouping BV 3 61.333 A ĐTK1 3 58.667 A B YB4 3 54.483 A B YB11 3 53.793 B Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of cong thuc Simultaneous confidence level = 82.43% cong thuc = BV subtracted from: cong thuc Lower Center Upper -+---------+---------+---------+-------- ĐTK1 -10.016 -2.667 4.682 (---------*----------) YB11 -14.889 -7.540 -0.191 (---------*----------) YB4 -14.199 -6.851 0.498 (---------*----------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 cong thuc = ĐTK1 subtracted from: cong thuc Lower Center Upper -+---------+---------+---------+-------- YB11 -12.222 -4.874 2.475 (---------*----------) YB4 -11.533 -4.184 3.165 (---------*----------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 cong thuc = YB11 subtracted from: cong thuc Lower Center Upper -+---------+---------+---------+-------- YB4 -6.659 0.690 8.039 (----------*---------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 Hiệu lực đối kháng các loại nấm 178 One-way ANOVA: 3NSC versus CT Source DF SS MS F P CT 3 196.06 65.35 15.17 0.001 Error 8 34.47 4.31 Total 11 230.54 S = 2.076 R-Sq = 85.05% R-Sq(adj) = 79.44% Individual 95% CIs For Mean Based on Pooled StDev Level N Mean StDev +---------+---------+---------+--------- C10 3 16.949 2.936 (------*------) Chae 1 3 8.475 0.000 (------*------) Chae 15 3 13.559 0.000 (------*------) ĐT13 3 6.780 2.936 (------*------) +---------+---------+---------+--------- 4.0 8.0 12.0 16.0 Pooled StDev = 2.076 Grouping Information Using Fisher Method CT N Mean Grouping C10 3 16.949 A Chae 15 3 13.559 A Chae 1 3 8.475 B ĐT13 3 6.780 B Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of CT Simultaneous confidence level = 82.43% CT = C10 subtracted from: CT Lower Center Upper +---------+---------+---------+-------- - Chae 1 -12.383 -8.475 -4.566 (-----*----) Chae 15 -7.298 -3.390 0.519 (----*-----) ĐT13 -14.078 -10.169 -6.261 (----*-----) +---------+---------+---------+-------- - -14.0 -7.0 0.0 7.0 CT = Chae 1 subtracted from: CT Lower Center Upper +---------+---------+---------+--------- Chae 15 1.176 5.085 8.993 (----*-----) ĐT13 -5.603 -1.695 2.214 (-----*----) +---------+---------+---------+--------- -14.0 -7.0 0.0 7.0 CT = Chae 15 subtracted from: CT Lower Center Upper +---------+---------+---------+--------- ĐT13 -10.688 -6.780 -2.871 (----*-----) +---------+---------+---------+--------- -14.0 -7.0 0.0 7.0 179 One-way ANOVA: 5NSC versus CT Source DF SS MS F P CT 3 171.79 57.26 9.00 0.006 Error 8 50.90 6.36 Total 11 222.69 S = 2.522 R-Sq = 77.14% R-Sq(adj) = 68.57% Individual 95% CIs For Mean Based on Pooled StDev Level N Mean StDev ---------+---------+---------+---------+ C10 3 53.398 0.000 (------*------) Chae 1 3 43.689 1.682 (-----*------) Chae 15 3 52.427 3.363 (------*------) ĐT13 3 49.515 3.363 (------*------) ---------+---------+---------+---------+ 45.0 50.0 55.0 60.0 Pooled StDev = 2.522 Grouping Information Using Fisher Method CT N Mean Grouping C10 3 53.398 A Chae 15 3 52.427 A ĐT13 3 49.515 A Chae 1 3 43.689 B Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of CT Simultaneous confidence level = 82.43% CT = C10 subtracted from: CT Lower Center Upper -+---------+---------+---------+-------- Chae 1 -14.458 -9.709 -4.959 (------*------) Chae 15 -5.720 -0.971 3.778 (------*-----) ĐT13 -8.633 -3.883 0.866 (-----*------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 CT = Chae 1 subtracted from: CT Lower Center Upper -+---------+---------+---------+-------- Chae 15 3.989 8.738 13.487 (-----*------) ĐT13 1.076 5.825 10.575 (-----*------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 CT = Chae 15 subtracted from: CT Lower Center Upper -+---------+---------+---------+-------- ĐT13 -7.662 -2.913 1.837 (------*------) -+---------+---------+---------+-------- -14.0 -7.0 0.0 7.0 180 One-way ANOVA: 7NSC versus CT Source DF SS MS F P CT 3 39.91 13.30 4.79 0.034 Error 8 22.21 2.78 Total 11 62.13 S = 1.666 R-Sq = 64.25% R-Sq(adj) = 50.84% Individual 95% CIs For Mean Based on Pooled StDev Level N Mean StDev ---------+---------+---------+---------+ C10 3 67.347 0.000 (-------*--------) Chae 1 3 62.585 1.178 (--------*--------) Chae 15 3 66.667 2.357 (--------*--------) ĐT13 3 65.306 2.041 (--------*--------) ---------+---------+---------+---------+ 62.5 65.0 67.5 70.0 Pooled StDev = 1.666 Grouping Information Using Fisher Method CT N Mean Grouping C10 3 67.347 A Chae 15 3 66.667 A ĐT13 3 65.306 A B Chae 1 3 62.585 B Means that do not share a letter are significantly different. Fisher 95% Individual Confidence Intervals All Pairwise Comparisons among Levels of CT Simultaneous confidence level = 82.43% CT = C10 subtracted from: CT Lower Center Upper +---------+---------+---------+--------- Chae 1 -7.899 -4.762 -1.624 (-------*-------) Chae 15 -3.818 -0.680 2.457 (-------*-------) ĐT13 -5.178 -2.041 1.097 (-------*-------) +---------+---------+---------+--------- -8.0 -4.0 0.0 4.0 CT = Chae 1 subtracted from: CT Lower Center Upper +---------+---------+---------+--------- Chae 15 0.944 4.082 7.219 (-------*-------) ĐT13 -0.416 2.721 5.859 (-------*-------) +---------+---------+---------+--------- -8.0 -4.0 0.0 4.0 CT = Chae 15 subtracted from: CT Lower Center Upper +---------+---------+---------+--------- ĐT13 -4.498 -1.361 1.777 (-------*------) +---------+---------+---------+--------- -8.0 -4.0 0.0 4.0 181 Hiệu lực của một số nguồn vi sinh vật đối kháng đến chủng Foc-TR4 trong điều kiện nhà lưới 30 ngày sau xử lý Randomized Complete Block AOV Table for CSB Source DF SS MS F P LN 2 0.016 0.0080 CT 4 139.860 34.9650 514.19 0.0000 Error 8 0.544 0.0680 Total 14 140.420 Grand Mean 8.3000 CV 3.14 Tukey's 1 Degree of Freedom Test for Nonadditivity Source DF SS MS F P Nonadditivity 1 0.00247 0.00247 0.03 0.8632 Remainder 7 0.54153 0.07736 Relative Efficiency, RCB 0.85 Means of CSB for CT CT Mean 1 6.200 2 7.700 3 6.200 4 7.100 5 14.300 Observations per Mean 3 Standard Error of a Mean 0.1506 Std Error (Diff of 2 Means) 0.2129 LSD All-Pairwise Comparisons Test of CSB for CT CT Mean Homogeneous Groups 5 14.300 A 2 7.700 B 4 7.100 C 1 6.200 D 3 6.200 D Alpha 0.05 Standard Error for Comparison 0.2129 Critical T Value 2.306 Critical Value for Comparison 0.4910 There are 4 groups (A, B, etc.) in which the means are not significantly different from one another. 45 ngày sau xử lý Randomized Complete Block AOV Table for CSB Source DF SS MS F P LN 2 0.028 0.0140 CT 4 272.220 68.0550 786.76 0.0000 Error 8 0.692 0.0865 Total 14 272.940 182 Grand Mean 7.7000 CV 3.82 Tukey's 1 Degree of Freedom Test for Nonadditivity Source DF SS MS F P Nonadditivity 1 0.17219 0.17219 2.32 0.1716 Remainder 7 0.51981 0.07426 Relative Efficiency, RCB 0.85 Means of CSB for CT CT Mean 1 5.500 2 5.500 3 5.200 4 6.100 5 16.200 Observations per Mean 3 Standard Error of a Mean 0.1698 Std Error (Diff of 2 Means) 0.2401 LSD All-Pairwise Comparisons Test of CSB for CT CT Mean Homogeneous Groups 5 16.200 A 4 6.100 B 1 5.500 C 2 5.500 C 3 5.200 C Alpha 0.05 Standard Error for Comparison 0.2401 Critical T Value 2.316 Critical Value for Comparison 0.5538 There are 3 groups (A, B, etc.) in which the means are not significantly different from one another. 60 ngày sau xử lý Randomized Complete Block AOV Table for CSB Source DF SS MS F P LN 2 0.444 0.222 CT 4 431.664 107.916 1401.51 0.0000 Error 8 0.616 0.077 Total 14 432.724 Grand Mean 6.9800 CV 3.98 Tukey's 1 Degree of Freedom Test for Nonadditivity Source DF SS MS F P Nonadditivity 1 0.00084 0.00084 0.01 0.9248 Remainder 7 0.61516 0.08788 Relative Efficiency, RCB 1.23 Means of CSB for CT CT Mean 1 3.200 183 2 4.200 3 4.300 4 5.600 5 17.600 Observations per Mean 3 Standard Error of a Mean 0.1602 Std Error (Diff of 2 Means) 0.2266 LSD All-Pairwise Comparisons Test of CSB for CT CT Mean Homogeneous Groups 5 17.600 A 4 5.600 B 3 4.300 C 2 4.200 C 1 3.200 D Alpha 0.05 Standard Error for Comparison 0.2266 Critical T Value 2.306 Critical Value for Comparison 0.5225 There are 4 groups (A, B, etc.) in which the means are not significantly different from one another.