Luận án Tiến sĩ Sinh học Nghiên cứu tạo chủng vi khuẩn vibrio parahaemolyticus đột biến giảm độc lực nhằm phát triển vắc - Xin phòng bệnh hoại tử gan thận trên một số loài cá biển

S.J., Song D., Chang Y., Jian J. (2018), "Construction of a Vibrio alginolyticus hopPmaJ (hop) mutant and evaluation of its potential as a live attenuated vaccine in orange-spotted 130 grouper (Epinephelus coioides)", Fish & Shellfish Immunology, 76, pp. 93- 100. 117. Paranjpye R.N., Myers M.S., Yount E.C., Thompson J.L. (2013), "Zebrafish as a model for Vibrio parahaemolyticus virulence", Microbiology, 159 (Pt 12), pp. 2605-2615. 118. Paria P., Chakraborty H.J., Behera B.K., Das Mohapatra P.K., Das B.K. (2019), "Computational characterization and molecular dynamics simulation of the thermostable direct hemolysin-related hemolysin (TRH) amplified from Vibrio parahaemolyticus", Microbial Pathogenesis, 127, pp. 172-182. 119. Park H.J., Oh S., Vinod N., Ji S., Noh H.B., Koo J.M., Lee S.H., Kim S.C., Lee K.S., Choi C.W. (2016), "Characterization of chemically-induced bacterial ghosts (BGs) using sodium hydroxide-induced Vibrio parahaemolyticus ghosts (VPGs)", International journal of molecular sciences, 17(11), pp. 1904-1919. 120. Pascual J., Macian M.C., Arahal D.R., Garay E., Pujalte M.J. (2010), "Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes", International journal of systematic and evolutionary microbiology, 60 (Pt 1), pp. 154-165. 121. Peng B., Lin X.P., Wang S.N., Yang M.J., Peng X.X., Li H. (2018), "Polyvalent protective immunogens identified from outer membrane proteins of Vibrio parahaemolyticus and their induced innate immune response.", Fish & Shellfish Immunology, 72, pp. 104-110. 122. Peng B., Ye J.Z., Han Y., Zeng L., Zhang J.Y., Li H. (2016), "Identification of polyvalent protective immunogens from outer membrane proteins in Vibrio parahaemolyticus to protect fish against bacterial infection.", Fish & Shellfish Immunology, 54, pp. 204-210. 131 123. Peramachi P. (2018), "Multi-subunit RNA polymerases of bacteria: An insight into their active sites and catalytic mechanism", Indian Journal of Science and Technology, 11 (39), pp. 1-37. 124. Perez-Varela M., Corral J., Vallejo J.A., Rumbo-Feal S., Bou G., Aranda J., Barbe J. (2017), "Mutations in the beta-cubunit of the RNA polymerase impair the surface-associated motility and virulence of Acinetobacter baumannii", Infection and Immunity, 85 (8), pp. e00327-00317. 125. Plant K.P., Lapatra S.E. (2011), "Advances in fish vaccine delivery", Developmental and Comparative Immunology, 35 (12), pp. 1256-1262. 126. Praja R.K. (2018), "The infection of Vibrio parahaemolyticus in shrimp and human", Oceana Biomedicina Journal, 1 (1), pp. 44-58. 127. Pridgeon J., Klesius P. (2013), "Development of live attenuated Streptococcus agalactiae as potential vaccines by selecting for resistance to sparfloxacin", Vaccine, 31 (24), pp. 2705-2712. 128. Pridgeon J., Klesius P., Yildirim-Aksoy M. (2013), "Attempt to develop live attenuated bacterial vaccines by selecting resistance to gossypol, proflavine hemisulfate, novobiocin, or ciprofloxacin", Vaccine, 31, pp. 2222-2230. 129. Pridgeon J.W., Klesius P.H. (2011), "Development of a novobiocin-resistant Edwardsiella ictaluri as a novel vaccine in channel catfish (Ictalurus punctatus)", Vaccine, 29, pp. 5631-5637. 130. Pridgeon J.W., Klesius P.H. (2011), "Development and efficacy of novobiocin and rifampicin-resistant Aeromonas hydrophila as novel vaccines in channel catfish and Nile tilapia", Vaccine, 29 (45), pp. 7896-7904. 131. Qazi O., Rahman H., Tahir Z., Qasim M., Khan S., Ahmad Anjum A., Yaqub T., Tayyab M., Ali N., Firyal S. (2014), "Mutation pattern in rifampicin resistance determining region of rpoB gene in multidrug-resistant Mycobacterium tuberculosis isolates from Pakistan", International Journal of Mycobacteriology, 3 (3), pp. 173-177. 132 132. Raghunath P. (2014), "Roles of thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) in Vibrio parahaemolyticus", Frontiers in Microbiology, 5, pp. 805-809. 133. Reed L.J., Muench H. (1938), "A simple method for estimating fifty percent endpoints", Am J Hyg, 27 (3), pp. 493-497. 134. Rojas M.V.R., Matté M.H., Dropa M., Silva M.L.D., Matté G.R. (2011), "Characterization of Vibrio parahaemolyticus isolated from oysters and mussels in São Paulo, Brazil", Revista do Instituto de Medicina Tropical de São Paulo, 53 (4), pp. 201-205. 135. Ryo S., Wijdeven R.H., Tyagi A., Hermsen T., Kono T., Karunasagar I., Rombout J.H., Sakai M., Verburg-van K.B.M., R. S. (2010), "Common carp have two subclasses of bony fish specific antibody IgZ showing differential expression in response to infection", Developmental and Comparative Immunology, 34(11), pp. 1183-1190. 136. Shefat S.H.T. (2018), "Vaccines for Use in Finfish Aquaculture", Acta Scientific Pharmaceutical Sciences, 2 (11), pp. 15-19. 137. Shoemaker C.A., Klesius P.H., Evans J.J. (2007), "Immunization of eyed channel catfish, Ictalurus punctatus, eggs with monovalent Flavobacterium columnare vaccine and bivalent F. columnare and Edwardsiella ictaluri vaccine", Vaccine, 25 (6), pp. 1126-1131. 138. Shyne A.P.S., Sobbhana K.S., George K.C., Paul R.R. (2008), "Phenotypic characteristics and antibiotic sensitivity of Vibrio parahaemolyticus strains isolated from diseases grouper (Epinephelus spp.)", J Mar Biol Ass, 50, pp. 1-6. 139. Shyne A.P.S., Sobhana K.S., George K.C., Paul R.R. (2016), "Selection of specific cell wall antigen for rapid detection of fish pathogenic Vibrio parahaemolyticus by enzyme immunoassay", Indian Journal of Fisheries, 63(2) (2), pp. 76-85. 133 140. Sommerset I., Krossoy B., Biering E., Frost P. (2005), "Vaccines for fish in aquaculture", Expert Rev Vaccines, 4 (1), pp. 89-101. 141. Subhashini N., Krishnaiah N., Bindu K.C. (2011), "Detection of Vibrio parahaemolyticus in shell fish by cultrural and polymerase chain reaction", International Journal of Pharma and Bio Sciences, 2, pp. 335-341. 142. Sudheesh P., Cain K. (2016), "Optimization of efficacy of a live attenuated Flavobacterium psychrophilum immersion vaccine.", Fish & Shellfish Immunology, 56, pp. 169-180. 143. Sun Y., Liu C., Sun L. (2010), "Isolation and analysis of the vaccine potential of an attenuated Edwardsiella tarda strain", Vaccine, 28(38), pp. 6344-6450. 144. Swain P., Behera T., Mohapatra D., Nanda P.K., Nayak S.K., Meher P.K., Das B.K. (2010), "Derivation of rough attenuated variants from smooth virulent Aeromonas hydrophila and their immunogenicity in fish", Vaccine, 28 (29), pp. 4626-4631. 145. Tarr C.L., Patel J.S., Puhr N.D., Sowers E.G., Bopp C.A., Strockbine N.A. (2007), "Identification of Vibrio isolates by a multiplex PCR assay and rpoB sequence determination", Journal of Clinical Microbiology, 45 (1), pp. 134- 140. 146. Temprano A., Riaño J., Yugueros J., González P., Castro L., Villena A., Luengo J.M., Naharro G. (2005), "Potential use of a Yersinia ruckeri O1 auxotrophic aroA mutant as a live attenuated vaccine.", J Fish Dis, 28(7), pp. 419-427. 147. Thuy N.T.T., Dung N.H., Wergeland H.I. (2013), "Specific humoral immune response and protection against Vibrio parahaemolyticus in orange-spotted grouper Epinephelus coioides", Int J Mar Sci, 4(1), pp. 24-35. 148. Titball R.W. (2008), "Vaccines against intracellular bacterial pathogens", Drug Discov Today, 13 (13-14), pp. 596-600. 134 149. Torresi M., Sperandii A., Ricci L., Prencipe V., Migliorati G., Pomilio F. (2018), "Detection and characterisation of potentially pathogenic species of Vibrio in the Vibrata river, Abruzzo region, Italy", Veterinaria Italiana, 54 (2), pp. 125-135. 150. Tuyet D.T., Thiem V.D., Von S.L., Chowdhury A., Park E., Canh D.G., Chien B.T., Van T.T., Naficy A., Rao M.R., Ali M., Lee H., Sy T.H., Nichibuchi M., Clemens J., Trach D.D. (2006), "Clinical, epidemiological, and socioeconomic analysis of an outbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam", Infect Dis 186(11), pp. 1615-1620. 151. Uribe C., Folch H., Enriquez R., Moran G. (2011), "Innate and adaptive immunity in teleost fish: a review", Veterinarni Medicina, 56(10), pp. 486- 503. 152. USDA A., LPS Agricultural Analytics Division for the USDA National Organic Program: Vaccines for Aquaculture. In.; 2014: 25 pp. 153. Vongxay K., Pan Z., Zhang X., Wang S., Cheng S., Mei L., Xu C., Fang W. (2008), "Occurrence of pandemic clones of Vibrio parahaemolyticus isolates from seafood and clinical samples in a Chinese coastal province", Foodborne Pathog Dis, 5(2), pp. 127-134. 154. Vyas V.K., Ukawala R.D., Ghate M., Chintha C. (2012), "Homology modeling a fast tool for drug discovery: current perspectives", Indian J Pharm Sci, 74 (1), pp. 1-17. 155. Wan Y., Liu C., Ma Q. (2019), "Structural analysis of a Vibrio phospholipase reveals an unusual Ser-His-chloride catalytic triad", The Journal of Biological Chemistry, 294(30), pp. 11391-11401. 156. Wang D., Yu S., Chen W., Zhang D., Shi X. (2010), "Enumeration of Vibrio parahaemolyticus in oyster tissues following artificial contamination and depuration", Lett Appl Microbiol, 51 (1), pp. 104-108. 157. Wang J., Zou L.L., Li A.X. (2014), "Construction of a Streptococcus iniae sortase A mutant and evaluation of its potential as an attenuated modified 135 live vaccine in Nile tilapia (Oreochromis niloticus)", Fish & Shellfish Immunology, 40(2), pp. 392-398. 158. Wang L., Shi L., Su J.Y., Ye Y.X., Zhong Q.P. (2013), "Detection of Vibrio parahaemolyticus in food samples using in situ loop-mediated isothermal amplification method", Gene, 515, pp. 421-425. 159. Whitaker W.B., Parent M.A., Naughton L.M., Richards G.P., Blumerman S.L., Boyd E.F. (2010), "Modulation of responses of Vibrio parahaemolyticus O3:K6 to pH and temperature stresses by growth at different salt concentrations", Applied and Environmental Microbiology, 76 (14), pp. 4720-4729. 160. Wilson M.R., Warr G.W. (1992), "Fish immunoglobulins and the genes that encode them", Annu Rev Fish Dis, 2, pp. 201-221. 161. Wise D., Greenway T., Byars T., Griffin M., Khoo L. (2015), "Oral vaccination of channel catfish against enteric septicemia of catfish using a live attenuated Edwardsiella ictaluri isolate.", J Aquat Anim Health, 27(2), pp. 135-143. 162. Wong H.C., Chen C.H., Chung Y.J., Liu S.H., Wang T.K., Lee C.L., Chiou C.S., Nishibuchi M., Lee B.K. (2005), "Characterization of new O3:K6 strains and phylogenetically related strains of Vibrio parahaemolyticus isolated in Taiwan and other countries", Journal of Applied Microbiology, 98 (3), pp. 572-580. 163. Wong H.C., S. H. Liu, L. W. Ku, I. Y. Lee, T. K. Wang, Y. S. Lee, C. L. Lee, L. P. Kuo, Shih D.Y. (2000), " Characterization of Vibrio parahaemolyticus isolates obtained from foodborne illness outbreaks during 1992 through 1995 in Taiwan", J Food Prot, 63, pp. 900-906. 164. Wu Z., Wang S. (2004), "Experimental study on immune protection of trivalent vaccine to common bacterial diseases of Pseudosciaena crocea", J Xiamen Univ, 43(1), pp. 112-118. 136 165. Xiao J., Chen T., Wang Q., Liu Q., Wang X., Lv Y., Wu H., Zhang Y. (2011), "Search for live attenuated vaccine candidate against Edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda", Lett Appl Microbiol, 53(4), pp. 430-437. 166. Xie Z.Y., Hu C.Q., Chen C., Zhang L.P., Ren C.H. (2005), "Investigation of seven Vibrio virulence genes among Vibrio alginolyticus and Vibrio parahaemolyticus strains from the coastal mariculture systems in Guangdong, China", Lett Appl Microbiol, 41 (2), pp. 202-207. 167. Xu X., Cheng J., Wu Q., Zhang J., Xie T. (2016), "Prevalence, characterization, and antibiotic susceptibility of Vibrio parahaemolyticus isolated from retail aquatic products in North China", BMC Microbiology, 16, pp. 32. 168. Xu X.J., Sang B.H., Chen W.B., Yan Q.P., Xiong Z.Y., Su J.B., Zou W.Z. (2015), "Intracellular survival of virulence and low-virulence strains of Vibrio parahaemolyticus in Epinephelus awoara macrophages and peripheral leukocytes", Genetics and Molecular Research : GMR, 14 (1), pp. 706-718. 169. Yanagihara I., Nakahira K., Yamane T., Kaieda S., Mayanagi K., Hamada D., Fukui T., Ohnishi K., Kajiyama S., Shimizu T., Sato M., Ikegami T., Ikeguchi M., Honda T., Hashimoto H. (2010), "Structure and functional characterization of Vibrio parahaemolyticus thermostable direct hemolysin", The Journal of Biological Chemistry, 285 (21), pp. 16267-16274. 170. Yáñez R., Bastías R., Higuera G., Salgado O., Katharios P., Romero J., Espejo R., García K. (2015), "Amplification of tlh gene in other Vibrionaceae specie by specie-specific multiplex PCR of Vibrio parahaemolyticus", Electron J Biotechnol, 18 (6), pp. 459-463. 171. Yeung P.S., Hayes M.C., DePaola A., Kaysner C.A., Kornstein L., Boor K.J. (2002), "Comparative phenotypic, molecular, and virulence characterization of Vibrio parahaemolyticus O3:K6 isolates", Appl Environ Microbiol, 68, pp. 2901-2909. 137 172. Yoon J.H., Bae Y.M., Lee S.Y. (2017), "Effects of varying concentrations of sodium chloride and acidic conditions on the behavior of Vibrio parahaemolyticus and Vibrio vulnificus cold-starved in artificial sea water microcosms", Food Science and Biotechnology, 26 (3), pp. 829-839. 173. Yu L., Hu Y., Sun B., Sun L. (2012), "C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine", Dis Aquat Organ, 102(1), pp. 33-42. 174. Zapata A., Diez B., Cejalvo T., Gutierrez-De Frias C., Cortes A. (2006), "Ontogeny of the immune system of fish", Fish & Shellfish Immunology, 20, pp. 126-136. 175. Zaw M.T., Emran N.A., Lin Z. (2018), "Mutations inside rifampicin- resistance determining region of rpoB gene associated with rifampicin- resistance in Mycobacterium tuberculosis", Journal of Infection and Public Health, 11 (5), pp. 605-610. 176. Zha Z., Li C., Li W., Ye Z., Pan J. (2016), "LptD is a promising vaccine antigen and potential immunotherapeutic target for protection against Vibrio species infection", Scientific Reports, 6, pp. 38577. 177. Zhang X., Mu Y., Mu P., Ao J., Chen X. (2017), "Transcriptome analysis reveals comprehensive insights into the early immune response of large yellow croaker (Larimichthys crocea) induced by trivalent bacterial vaccine", PloS one, 12 (1), pp. e0170958. 178. Zhang X.H., Austin B. (2005), "Haemolysins in Vibrio species", Journal of Applied Microbiology, 98 (5), pp. 1011-1019. 179. Zhang Y., Arias C., Shoemaker C., Klesius P. (2006), "Comparison of lipopolysaccharide and protein profiles between Flavobacterium columnare strains from different genomovars", J Fish Dis, 29, pp. 657-663. 180. Zhang Y., Hu L., Osei-Adjei G., Zhang Y., Yang W., Yin Z., Lu R., Sheng X., Yang R., Huang X., Zhou D. (2018), "Autoregulation of toxR and its 138 regulatory actions on major virulence gene loci in Vibrio parahaemolyticus", Frontiers in Cellular and Infection Microbiology, 8, pp. 291. 181. Zhou S., Gao Z.X., Zhang M., Liu D.Y., Zhao X.P., Liu Y. (2016), "Development of a quadruplex loop-mediated isothermal amplification assay for field detection of four Vibrio species associated with fish disease", SpringerPlus, 5 (1), pp. 1104. 182. Zulkifli Y., Alitheen N.B., Son R., Yeap S.K., Lesley M.B., Raha A.R. (2009), "Identification of Vibrio parahaemolyticus isolates by PCR targeted to the toxR gene and detection of virulence genes", International Food Research Journal, 16, pp. 289-296. 183. https://www.gso.gov.vn/default.aspx?tabid=382&idmid=2&ItemID=19036 184. https://tongcucthuysan.gov.vn/portals/0/bn%20tin%20thy%20sn%2027- 07%20-%202017.pdf 185. https://tongcucthuysan.gov.vn/portals/0/bn%20tin%20thy%20sn%20tin%no ng%2017-08-2017.pdf 186. https://bnews.vn/da-tim-ra-nguyen-nhan-ca-chet-tren-song-cha- va/53608.html 187. https://www.gso.gov.vn/default.aspx?tabid=621&ItemID=19037 139 PHỤ LỤC Phụ lục 1: Trình tự gen toxR của các chủng vi khuẩn Chủng Trình tự gen toxR LBT6 ATGACTAACATCGGCACCAAATTTCTACTTGCTCAAAGGTTTACC TTTGATCCAAATAGTAATTCGCTCGCTGACCAACAAAGCGGCAA CGAAGTTGTACGATTAGGAAGCAACGAAAGCCGTATACTCCTGA TGTTGGCGGAGAGACCAAACGAAGTTTTAACCCGTAACGAGCTT CACGAGTTTGTTTGGCGTGAGCAAGGTTTTGAGGTGGATGACTC AAGCCTGACTCAAGCGATTTCTACTCTGCGTAAGATGTTGAAGG ATTCAACCAAATCTCCAGAGTTTGTTAAAACCGTTCCAAAACGA GGCTATCAACTCATTTGTACTGTTGAACGCCTAAGCCCGCTTTCT TCAGACTCAAGCTCAATTGAAGTTGAAGAACCTGCTTCTGATAA CAATGACGCCTCTGCTAATGAGGTAGAAACGATCGTAGAGCCGC CTTTAGCGACGTCTTCTGACGCAATCGTTGAACCAGAAGCGCCA GTAGTACCTGAAAAAGCACCTGTGGCTTCTGCTGTGAATCCTTG GATTCCACGCGTTATTTTATTTTTGGCACTATTACTACCGATTTG CGTACTGCTGTTTACAAACCCAGCGGAATCTCAGTTCCGTCAGA TTGGTGAGTATCAGAACGTACCAGTGATGACACCTGTAAATCAC CCGCAAATCAACAACTGGCTGCCTTCTATTGAGCAGTGCATTGA ACGCTACGTTAAGCACCATGCAGAAGACTCGTTACCAGTGGAAG TGATTGCCACTGGCGGACAAAATAACCAGCTGATTTTGAACTAC ATTCATGACAGTAACCACTCGTATGAGAACGTGACATTGCGTAT TTTCGCAGGTCAAAATGATCCAACAGATATCTGCAAATAA A3.3 ATGACTAACATCGGCACCAAATTTCTACTTGCTCAAAGGTTTACC TTTGATCCAAATAGTAATTCGCTCGCTGACCAACAAAGCGGCAA CGAAGTTGTACGATTAGGAAGCAACGAAAGCCGTATACTCCTGA TGTTGGCGGAGAGACCAAACGAAGTTTTAACCCGTAACGAGCTT CACGAGTTTGTTTGGCGTGAGCAAGGTTTTGAGGTGGATGACTC AAGCCTGACTCAAGCGATTTCTACTCTGCGTAAGATGTTGAAGG ATTCAACCAAATCTCCAGAGTTTGTTAAAACCGTTCCAAAACGA GGCTATCAACTCATTTGTACTGTTGAACGCCTAAGCCCGCTTTCT TCAGACTCAAGCTCAATTGAAGTTGAAGAACCTGCTTCTGATAA CAATGACGCCTCTGCTAATGAGGTAGAAACGATCGTAGAGCCGC CTTTAGCGACGTCTTCTGACGCAATCGTTGAACCAGAAGCGCCA GTAGTACCTGAAAAAGCACCTGTGGCTTCTGCTGTGAATCCTTG GATTCCACGCGTTATTTTATTTTTGGCACTATTACTACCGATTTG 140 CGTACTGCTGTTTACAAACCCAGCGGAATCTCAGTTCCGTCAGA TTGGTGAGTATCAGAACGTACCAGTGATGACACCTGTAAATCAC CCGCAAATCAACAACTGGCTGCCTTCTATTGAGCAGTGCATTGA ACGCTACGTTAAGCACCATGCAGAAGACTCGTTACCAGTGGAAG TGATTGCCACTGGCGGACAAAATAACCAGCTGATTTTGAACTAC ATTCATGACAGTAACCACTCGTATGAGAACGTGACATTGCGTAT TTTCGCAGGTCAAAATGATCCAACAGATATCTGCAAATAA B3.13 ATGACTAACATCGGCACCAAATTTCTACTTGCTCAAAGGTTTACC TTTGATCCAAATAGTAATTCGCTCGCTGACCAACAAAGCGGCAA CGAAGTTGTACGATTAGGAAGCAACGAAAGCCGTATACTCCTGA TGTTGGCGGAGAGACCAAACGAAGTTTTAACCCGTAACGAGCTT CACGAGTTTGTTTGGCGTGAGCAAGGTTTTGAGGTGGATGACTC AAGCCTGACTCAAGCGATTTCTACTCTGCGTAAGATGTTGAAGG ATTCAACCAAATCTCCAGAGTTTGTTAAAACCGTTCCAAAACGA GGCTATCAACTCATTTGTACTGTTGAACGCCTAAGCCCGCTTTCT TCAGACTCAAGCTCAATTGAAGTTGAAGAACCTGCTTCTGATAA CAATGACGCCTCTGCTAATGAGGTAGAAACGATCGTAGAGCCGC CTTTAGCGACGTCTTCTGACGCAATCGTTGAACCAGAAGCGCCA GTAGTACCTGAAAAAGCACCTGTGGCTTCTGCTGTGAATCCTTG GATTCCACGCGTTATTTTATTTTTGGCACTATTACTACCGATTTG CGTACTGCTGTTTACAAACCCAGCGGAATCTCAGTTCCGTCAGA TTGGTGAGTATCAGAACGTACCAGTGATGACACCTGTAAATCAC CCGCAAATCAACAACTGGCTGCCTTCTATTGAGCAGTGCATTGA ACGCTACGTTAAGCACCATGCAGAAGACTCGTTACCAGTGGAAG TGATTGCCACTGGCGGACAAAATAACCAGCTGATTTTGAACTAC ATTCATGACAGTAACCACTCGTATGAGAACGTGACATTGCGTAT TTTCGCAGGTCAAAATGATCCAACAGATATCTGCAAATAA Phụ lục 2: Trình tự gen tlh của các chủng/dòng vi khuẩn Chủng/ dòng Trình tự gen tlh LBT6 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC 141 ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA L3900 ATGAAAAAAACAATCACACTATTAACTGCATTATTCCCGGTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGGTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC 142 AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTCTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA L4200 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT 143 GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAAAT TAGCCGCAGTACCGTTCATA L4650 ATGAAAAAAACAATCCCACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAAAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGTTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCATCAAAGTGGTT GCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACGC ATCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGTC ACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGCG AAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGCGCGGCTGG TGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAAGTTT CTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACCA GCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCATG AACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAGA AGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATGT TGATGACGGTGCCAGATGTGACGTAAGCACCTCAGTTTAAGTAC TCACCACAAGAAGAGATCGACAAAATTCGTGAGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGCTGTTTGATACTCACGCCCTGTTTGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCAGCGTCTGGTGCT GAGAAGTTTGTGTTCTGGGATGTCACGCATCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCAGAGTACCGTTTCTAA A3.3 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC 144 ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCAA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA A400 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC 145 AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGACCACGGTTTCGTGAACGCAA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCAATTACA TGGACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCTCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA A650 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAAAAAACACGGTTTCGTGAACGCAAG TGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACAT GTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCTG 146 AGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAACT CACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACTT AGCACGAGTAACCGTTTAAT B3.13 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B5700 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC 147 ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B7050 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTATACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC 148 AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTGAATGACTTCAT GAACTACAACCGTGGCTTTCCAGAAGTGAAAGCGGAATATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCAACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA B7900 ATGAAAAAAACAATCACACTATTAACTGCATTACTCCCGCTTGC TTCTGCAGTTGCCGAAGAGCCAACCTTATCACCAGAAATGGTTT CAGCGTCTGAAGTGATCAGCACGCAAGAAAACCAAACCTATACC TATGTTCGCTGTTGGTATCGCACCAGCTACTCGAAAGATGATCC GGCGACCGATTGGGAATGGGCAAAAAACGAAGATGGTAGCTAC TTCACCATTGACGGCTACTGGTGGAGCTCCGTTTCATTTAAAAAC ATGTTCTACACCAACACGTCGCAAAACGTTATCCGTCAGCGTTG TGAAGCAACATTAGATTTGGCGAACGAGAACGCAGACATTACGT TCTTCGCCGCTGACAATCGCTTCTCATACAACCACACGATCTGGA GCAACGACGCAGCAATGCAGCCAGATCAAATCAACAAAGTGGT TGCACTCGGTGACAGCTTGTCTGATACAGGCAACATCTTTAACG CGTCACAATGGCGCTTCCCTAACCCGAACAGCTGGTTCTTAGGT CACTTCTCCAACGGTTTTGTGTGGACAGAATACATTGCCAAAGC GAAGAACCTTCCGCTCTACAACTGGGCAGTTGGCGGTGCGGCTG GTGAGAACCAATACATCGCGCTAACAGGGGTTGGTGAGCAGGTT TCTTCGTACTTAACCTACGCAAAACTGGCGAAGAACTACAAACC AGCAAACACCTTGTTTACGCTTGAGTTTGGTTTTAATGACTTCAT GAACTACAACCGTGGCGTTCCAGAAGTGAAAGCGGATTATGCAG AAGCACTGATTCGTTTGACGGACGCAGGTGCGAAGAACTTCATG TTGATGACACTGCCAGATGCGACGAAAGCGCCTCAGTTTAAGTA CTCAACACAAGAAGAGATCGACAAAATTCGTGCGAAAGTGCTTG AGATGAACGAGTTCATCAAGGCACAAGCGATGTACTACAAAGC GCAAGGTTACAACATCACGTTGTTTGATACTCACGCCTTGTTCGA GACGCTAACTTCTGCGCCAGAAGAGCACGGTTTCGTGAACGCGA GTGATCCTTGTTTGGACATCAACCGCTCATCGTCTGTCGATTACA TGTACACCCACGCATTGCGCTCTGAGTGTGCGGCGTCCGGTGCT 149 GAGAAATTTGTGTTCTGGGATGTCACGCACCCAACAACAGCAAC TCACCGCTATGTTGCAGAGAAAATGCTAGAAAGTAGCAACAACT TAGCCGAGTACCGTTTCTAA Phụ lục 3. Trình tự chuỗi polypeptide suy diễn từ gen tlh các chủng/dòng vi khuẩn Chủng/ dòng Trình tự polypeptide suy diễn các chủng/dòng vi khuẩn LBT6 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* L3900 MKKTITLLTALFPVASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* L4200 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV 150 DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NKLAAVPFI L4650 MKKTIPLLTALLPLASAVAEKPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQIIKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTVPDVT*APQFKYSPQEEIDKIREKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* A3.3 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* A400 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEDHGFVNASDPCLDINRSSS VNYMDTHALRSECAASGAEKFVFWDVTHPTTATHLYVAEKMLES SNNLAEYRF* A650 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA 151 MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPKNTVS*TQVILVWTSTAHRL SITCTPTHCALSVRRPVLRNLCSGMSRTQQQQLTAMLQRKC*KVAT T*HE*PFN B3.13 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* B7050 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMLY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGLNDFMNYNRGFPEVKAEYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS NNLAEYRF* B7900 MKKTITLLTALLPLASAVAEEPTLSPEMVSASEVISTQENQTYTYVR CWYRTSYSKDDPATDWEWAKNEDGSYFTIDGYWWSSVSFKNMFY TNTSQNVIRQRCEATLDLANENADITFFAADNRFSYNHTIWSNDAA MQPDQINKVVALGDSLSDTGNIFNASQWRFPNPNSWFLGHFSNGFV WTEYIAKAKNLPLYNWAVGGAAGENQYIALTGVGEQVSSYLTYA KLAKNYKPANTLFTLEFGFNDFMNYNRGVPEVKADYAEALIRLTD AGAKNFMLMTLPDATKAPQFKYSTQEEIDKIRAKVLEMNEFIKAQA MYYKAQGYNITLFDTHALFETLTSAPEEHGFVNASDPCLDINRSSSV DYMYTHALRSECAASGAEKFVFWDVTHPTTATHRYVAEKMLESS 152 NNLAEYRF* Phụ lục 4: Trình tự nucleotide đoạn gen rpoB của chủng/dòng vi khuẩn Chủng/ dòng Trình tự nucleotide đoạn gen rpoB của chủng/dòng vi khuẩn LBT6 AGCCACCAACAAAAGAAGCTGCAGAAGCACTATTCGAAAGCCT ATTCTTCTCTGAAGAGCGTTACGATCTATCGACTGTTGGTCGTAT GAAGTTTAATAGCTCTATCGGTCGTGAAGATGCTCAAGAGCAAG GCACACTTGATGAAACAGATATCATCGAAGTGATGAAGAAGCTT ATCGCGATCCGTAACGGCAAAGGCGAAGTGGACGATATCGACCA CCTAGGCAACCGTCGTATCCGTTCTGTCGGCGAAATGGCTGAGAA CCAGTTCCGTGTAGGTCTAGTACGTGTTGAGCGTGCAGTTAAAGA GCGTCTAAGCCTAGGCGATCTTGACGCAATCATGCCTCAAGACCT GATCAACGCGAAGCCAATTTCTGCAGCGGTTAAAGAATTCTTTGG CTCTTCACAGCTTTCACAGTTCATGGACCAAAACAACCCGCTGTC AGAAGTAACGCACAAGCGTCGTATTTCTGCATTGGGTCCTGGCGG TCTGACTCGTGAACGTGCTGGCTTCGAAGTTCGAGACGTACACGT AACTCACTACGGTCGTCTATGTCCTATCGAAACGCCGGAAGGTCC AAACATCGGTCTGATCAACTCTCTATCTGCGTTTGCGCGTTGTAA CGAGTACGGTTTCCTTGAGACGCCATACCGTCGCGTTGTAGATGG TGTTGTAACAGACGAAGTAGATTACCTATCTGCAATCGAAGAAG GCCAATTCGTTATCGCTCAGGCGAACGCTAAGCTAAACGAAGAT GGTACTTTTGCAGATGAGCTGATCACAGCTCGTCAGAAAGGTGA ATCTGGTCTACACCCTCGTGAGCACGCTCAGTACATGGACGTTGC GACAAACCAGGTTGTATCTATCGCTGCATCGCTTATCCCGTTCCT AGAACACGATGATGCGAACCGTGCATTGATGGGTGCGA L4650 ACTATTCGAAAACCTGTTCTTCTCCGAAGACCGCTACGATCTCTC CGCTGTTGGTCGTATGAAGTTCAACAGCTCTATCGGTCGTGACG ATGCTGAAGAGCAAGGTACACTGGATAAAGAAGACATCATCGA AGTGATGAAGAAGCTCATCGATATCCGTAACGGTAAAGGCGAA GTGGACGATATCGACCACCTCGGCAACCGTCGTATCCGTTCCGT AGGCGAAATGGCGGAAAACCAGTTCCGCGTTGGCCTGGTACGTG TAGAGCGTGCGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGAT GCAATCATGCCTCAGGATATGATCAACGCCAAGCCGATCTCCGC AGCAGTGAAAGAGTTCTTTGGTTCCAGCCAGCTGTCTCAGTTTAT GGACCAGAACAACCCGCTGTCTGAGATCACGCACAAGCGTCGTA TCTCTGCACTCGGCCCAGGCGGTCTGACCCGTGAGCGCGCAGGC 153 TTCGAAGTTCGAGACGTACACGTAACCCACTACGGTCGCGTATG TCCAATCGAAACGCCTGAAGGTCCAAACATCGGTCTGATCAACT CCCTGTCCGTGTACGCACGTTGTAACGAATACGGCTTCCTCGAG ACCCCGTACCGTCGCGTGGTAGACGGTGTTGTAACCGACGAAGT ACACTACCTGTCTGCTATCGAAGAAGGTCAATACGTTATCGCCC AGGCGAACACCAACCTGGACGAAGAAGGTACTTTTGCAGATGA CCTCGTTACAGCCCGTCAGAAAGGTGAATCCAGCTTACTCCCTC GTGACCAGGTTCAGTACATGGACGTTTCCACCAACCAGGTGGTT TCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGC CAACC B3.13 ATCTACCGTATGATGCGCCCTGGTGAGCCACCAACAAAAGAAGC TGCAGAAGCACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTT ACGATCTATCGACTGTTGGTCGTATGAAGTTTAATAGCTCTATCG GTCGTGAAGATGCTCAAGAGCAAGGCACACTTGATGAAACAGA TATCATCGAAGTGATGAAGAAGCTTATCGCGATCCGTAACGGCA AAGGCGAAGTGGACGATATCGACCACCTAGGCAACCGTCGTATC CGTTCTGTCGGCGAAATGGCTGAGAACCAGTTCCGTGTAGGTCT AGTACGTGTTGAGCGTGCAGTTAAAGAGCGTCTAAGCCTAGGCG ATCTTGACGCAATCATGCCTCAAGACCTGATCAACGCGAAGCCA ATTTCTGCAGCGGTTAAAGAATTCTTTGGCTCTTCACAGCTTTCA CAGTTCATGGACCAAAACAACCCGCTGTCAGAAGTAACGCACAA GCGTCGTATTTCTGCATTGGGTCCTGGCGGTCTGACTCGTGAACG TGCTGGCTTCGAAGTTCGAGACGTACACGTAACTCACTACGGTC GTCTATGTCCTATCGAAACGCCGGAAGGTCCAAACATCGGTCTG ATCAACTCTCTATCTGCGTTTGCGCGTTGTAACGAGTACGGTTTC CTTGAGACGCCATACCGTCGCGTTGTAGATGGTGTTGTAACAGA CGAAGTAGATTACCTATCTGCAATCGAAGAAGGCCAATTCGTTA TCGCTCAGGCGAACGCTAAGCTAAACGAAGATGGTACTTTTGCA GATGAGCTGATCACAGCTCGTCAGAAAGGTGAATCTGGTCTACA CCCTCGTGAGCACGCTCAGTACATGGACGTTGCGACAAACCAGG TTGTATCTATCGCTGCATCGCTTATCCCGTTCCTAGAACACGATG ATGCGAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCA GTTCCAA A3.3 TGATGCGCCCTGGTGAGCCACCAACAAAAGAAGCTGCAGAAGC ACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTTACGATCTATC GACTGTTGGTCGTATGAAGTTTAATAGCTCTATCGGTCGTGAAG ATGCTCAAGAGCAAGGCACACTTGATGAAACAGATATCATCGAA GTGATGAAGAAGCTTATCGCGATCCGTAACGGCAAAGGCGAAGT GGACGATATCGACCACCTAGGCAACCGTCGTATCCGTTCTGTCGG 154 CGAAATGGCTGAGAACCAGTTCCGTGTAGGTCTAGTTCGTGTTGA GCGTGCAGTTAAAGAGCGTCTGAGTCTGGGCGACCTTGATGCGAT CATGCCTCAAGACCTGATCAATGCGAAACCAATTTCTGCAGCGGT TAAAGAATTCTTTGGCTCTTCACAGCTTTCACAGTTCATGGACCA AAACAACCCGTTATCAGAAGTAACGCACAAGCGTCGTATTTCTGC ATTGGGTCCTGGCGGTCTAACTCGTGAACGTGCTGGCTTCGAAGT TCGAGACGTACACGTAACTCACTACGGCCGTCTATGTCCTATCGA AACGCCGGAAGGTCCAAACATCGGTCTGATCAACTCTCTATCTGC GTTTGCGCGTTGTAACGAGTACGGTTTCCTTGAGACGCCATACCG TCGCGTTGTAGATGGTGTTGTAACAGACGAAGTAGATTACCTATC TGCAATCGAAGAAGGCCAATTCGTTATCGCTCAGGCGAACGCTA AGCTAAACGAAGATGGTACTTTTGCAGATGAGCTGATCACAGCTC GTCAGAAAGGTGAATCTGGTCTACATCCTCGTGAGCACGCTCAGT ACATGGACGTTGCGACAAACCAGGTTGTATCTATCGCTGCATCGC TTATCCCGTTCCTAGAACACGATGATGCGAACC A650 TGATGCGCCCTGGTGAGCCACCAACAAAAGAAGCTGCAGAAGC ACTATTCGAAAGCCTATTCTTCTCTGAAGAGCGTTACGATCTATC GACTGTTGGTCGTATGAAGTTTAATAGCTCTATCGGTCGTGAAG ATGCTCAAGAGCAAGGCACACTTGATGAAACAGATATCATCGAA GTGATGAAGAAGCTTATCGCGATCCGTAACGGCAAAGGCGAAG TGGACGATATCGACCACCTAGGCAACCGTCGTATCCGTTCTGTC GGCGAAATGGCTGAGAACCAGTTCCGTGTAGGTCTAGTACGTGT TGAGCGTGCAGTTAAAGAGCGTCTAAGCCTAGGCGATCTTGACG CAATCATGCCTCAAGACCTGATCAACGCGAAGCCAATTTCTGCA GCGGTTAAAGAATTCTTTGGCTCTTCACAGCTTTCACCGTTCATG GACCAAAACAACCCGCTGTCAGAAGTAACGCACAAGCGTCGTAT TTCTGCATTGGGTCCTGGCGGTCTGACTCGTGAACGTGCTGGCTT CGAAGTTCGAGACGTACACGTAACTCACTACGGTCGTCTATGTC CTATCGAAACGCCGGAAGGTCCAAACATCGGTCTGATCAACTCT CTATCTGCGTTTGCGCGTTGTAACGAGTACGGTTTCCTTGAGACG CCATACCGTCGCGTTGTAGATGGTGTTGTAACAGACGAAGTAGA TTACCTATCTGCAATCGAAGAAGGCCAATTCGTTATCGCTCAGG CGAACGCTAAGCTAAACGAAGATGGTACTTTTGCAGATGAGCTG ATCACAGCTCGTCAGAAAGGTGAATCTGGTCTACACCCTCGTGA GCACGCTCAGTACATGGACGTTGCGACAAACCAGGTTGTATCTA TCGCTGCATCGCTTATCCCGTTCCTAGAACACGATGATGCGAAC C 155 Phụ lục 5: Trình tự chuỗi polypeptide suy diễn từ đoạn gen rpoB Chủng/ dòng Trình tự chuỗi polypeptide suy diễn từ đoạn gen rpoB LBT6 PGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQEQG TLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAENQFR VGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSSQLS QFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHYGR LCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTDEVD YLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPREHA QYMDVATNQVVSIAASLIPFLEHDDANRALMGANMHR L4650 LFENLFFSEDRYDLSAVGRMKFNSSIGRDDAEEQGTLDKEDIIEVMK KLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVK ERLSLGDLDAIMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSE ITHKRRISALGPGGLTRERAGFEVRDVHVTHYGRVCPIETPEGPNIG LINSLSVYARCNEYGFLETPYRRVVDGVVTDEVHYLSAIEEGQYVI AQANTNLDEEGTFADDLVTARQKGESSLLPRDQVQYMDVSTNQV VSVGASLIPFLEHDDAN A3.3 MRPGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQE QGTLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAEN QFRVGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSS QLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTH YGRLCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTD EVDYLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPRE HAQYMDVATNQVVSIAASLIPFLEHDDAN A650 MRPGEPPTKEAAEALFESLFFSEERYDLSTVGRMKFNSSIGREDAQE QGTLDETDIIEVMKKLIAIRNGKGEVDDIDHLGNRRIRSVGEMAEN QFRVGLVRVERAVKERLSLGDLDAIMPQDLINAKPISAAVKEFFGSS QLSPFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHVTHY GRLCPIETPEGPNIGLINSLSAFARCNEYGFLETPYRRVVDGVVTDE VDYLSAIEEGQFVIAQANAKLNEDGTFADELITARQKGESGLHPRE HAQYMDVATNQVVSIAASLIPFLEHDDAN 156 Phụ lục 6: So sánh trình tự gen toxR của chủng V. parahaemolyticus A3.3 và B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus CDC_K4557 1618 100 0,0 99,89 CP006008.1 2 V. parahaemolyticus BB22OP 1618 100 0,0 99,89 CP003972.1 3 V. parahaemolyticus FORC_072 1591 100 0,0 99,32 CP013472.1 4 V. parahaemolyticus R13 1591 100 0,0 99,32 CP028342.1 5 V. parahaemolyticus R14 1591 100 0,0 99,32 CP028341.1 6 V. parahaemolyticus toxR and toxS genes 1591 100 0,0 99,32 L11929.1 7 V. parahaemolyticus MVP1 1585 100 0,0 99,20 CP043421.1 8 V. parahaemolyticus ATCC 17802 1585 100 0,0 99,20 AY527396.1 50 V. parahaemolyticus S107-1 1552 100 0,0 98,52 CP028481.1 100 V. parahaemolyticus Vp2010-008 toxR 948 60 0,0 98,87 KT194122.1 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus CDC_K4557 1618 100 0,0 99,89 CP006008.1 2 V. parahaemolyticus BB22OP 1618 100 0,0 99,89 CP003972.1 3 V. parahaemolyticus FORC_072 1591 100 0,0 99,32 CP013472.1 4 V. parahaemolyticus R13 1591 100 0,0 99,32 CP028342.1 5 V. parahaemolyticus R14 1591 100 0,0 99,32 CP028341.1 6 V. parahaemolyticus toxR and toxS genes 1591 100 0,0 99,32 L11929.1 7 V. parahaemolyticus MVP1 1585 100 0,0 99,20 CP043421.1 8 V. parahaemolyticus ATCC 17802 1585 100 0,0 99,20 AY527396.1 157 50 V. parahaemolyticus FORC-004 1552 100 0,0 98,52 CP028481.1 100 V. parahaemolyticus Vp2010-068 toxR 948 60 0,0 98,87 KT194123.1 Phụ lục 7: So sánh trình tự gen tlh của chủng V. parahaemolyticus A3.3, B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FORC_006 2300 100 0,0 99,76 CP009766.1 2 V. parahaemolyticus FORC-023 CP012951.1 3 V. parahaemolyticus 160807 2278 100 0,0 99,44 CP033142.1 4 V. parahaemolyticus FORC-071 2272 100 0,0 99,36 CP023486.1 5 V. parahaemolyticus UCM-V493 2266 100 0,0 99,28 CP007005.1 6 V. parahaemolyticus MVP1 2255 100 0,0 99,12 CP043422.1 7 V. parahaemolyticus S107-1 2255 100 0,0 99,12 CP028482.1 8 V. parahaemolyticus VH12 2255 100 0,0 99,12 KT948112.1 9 V. parahaemolyticus Vp-18 2255 100 0,0 99,12 JF513048.1 10 V. parahaemolyticus Vb0624 2250 100 0,0 99,04 CP041201.1 44 V. parahaemolyticus 1682 1552 100 0,0 89,00 CP012095.1 97 V. parahaemolyticus TBW-9-11-1 802 35 0,0 98,89 JX262952.1 100 V. harveyi VIB647 800 92 0,0 79,13 DQ224369.1 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FORC_006 2300 100 0,0 99,76 CP009766.1 2 V. parahaemolyticus FORC-023 CP012951.1 158 3 V. parahaemolyticus 160807 2278 100 0,0 99,44 CP033142.1 4 V. parahaemolyticus FORC-071 2272 100 0,0 99,36 CP023486.1 5 V. parahaemolyticus UCM-V493 2266 100 0,0 99,28 CP007005.1 6 V. parahaemolyticus MVP1 2261 100 0,0 99,20 CP031782.1 7 V. parahaemolyticus VPD14 2261 100 0,0 99,20 CP028482.1 8 V. parahaemolyticus 20130629002S01 2261 100 0,0 99,20 CP028482.1 42 V. parahaemolyticus VP1.1997 2135 94 0,0 99,16 KT948114.1 97 V. parahaemolyticus TBW-9-11-1 808 35 0,0 99,11 JX262952.1 100 V. diabolicusi JPW-8-11-8 802 35 0,0 98,89 JX262986.1 Phụ lục 8: So sánh trình tự gen rpoB của chủng V. parahaemolyticus A3.3, B3.13 với các trình tự trên NCBI bằng công cụ BLAST Chủng A3.3 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus FDAARGOS_51 1701 100 0,0 99,78 CP026041.1 2 V. parahaemolyticus MAVP-26 1701 100 0,0 99,78 CP0232248.1 3 V. parahaemolyticus CHN25 1690 100 0,0 99,57 CP010883.1 4 V. parahaemolyticus HA2 1685 100 0,0 99,46 CP023710.1 5 V. parahaemolyticus VN-10092 1657 100 0,0 98,92 KJ647551.1 6 V. parahaemolyticus ATCC 17802 1688 99 0,0 98,64 CP014046.1 7 V. parahaemolyticus BB22OP 1640 100 0,0 98,60 CP003972.1 50 V. parahaemolyticus VN-2815 1629 100 0,0 98,38 KJ647561.1 100 V. parahaemolyticus VN-4027 1616 100 0,0 98,06 KJ648147.1 159 Chủng B3.13 STT Chủng Tổng số điểm Tỉ lệ che phủ (%) Giá trị kì vọng Độ tương đồng (%) Số hiệu gen 1 V. parahaemolyticus ATCC17802 1792 100 0,0 99,69 CP014046.2 2 V. parahaemolyticus UCM-V493 1792 100 0,0 99,69 CP007004.1 3 V. parahaemolyticus FORC_006 1781 100 0,0 94,49 CP009765.1 4 V. parahaemolyticus VN-3112 1764 98 0,0 99,69 KJ647744.1 5 V. parahaemolyticus 17802 1764 98 0,0 99,69 EU909181.1 6 V. parahaemolyticus VN-4004 1755 98 0,0 98,48 JK647843.1 50 V. parahaemolyticus SO150 1703 100 0,0 98,06 HQ533927.1 100 V. parahaemolyticus VN-4019 1679 98 0,0 98,02 KJ648140.1 Phụ lục 9: Cấu trúc bậc 2 của TLH ở các chủng/dòng vi khuẩn 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179